Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (12% Tris Glycine)
Sample
Human Cell lysate - whole cell (U2OS and HeLa cells)
Specification
U2OS and HeLa cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk in PBS
Dilution
1/2500
Secondary antibody
Name
Non-Abcam antibody was used: KPL
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
SuperSignal Pico
Bands
Specific: 70 kDa
Exposure
2 minute(s) and 0 second(s)
Additional data
Additional Notes
Strong Antibody. Left lane U2OS cell lysate, right lane HeLa cell lysate.
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提交于 Jan 18 2011