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Signal Transduction Protein Trafficking Chaperones Heat Shock Proteins
重组

重组Anti-Hsp70抗体[3A3] (ab5439)

  • Datasheet
  • SDS
Reviews (3)Q&A (6)References (44)

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Western blot - Anti-Hsp70 antibody [3A3] (ab5439)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [3A3] (ab5439)
  • Immunocytochemistry - Anti-Hsp70 antibody [3A3] (ab5439)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [3A3] (ab5439)
  • Immunocytochemistry - Anti-Hsp70 antibody [3A3] (ab5439)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [3A3] (ab5439)
  • Immunocytochemistry - Anti-Hsp70 antibody [3A3] (ab5439)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [3A3] (ab5439)
  • Anti-Hsp70 antibody [3A3] (ab5439)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [3A3] to Hsp70
  • Suitable for: ICC, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

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概述

  • 产品名称

    Anti-Hsp70抗体[3A3]
    参阅全部 Hsp70 一抗
  • 描述

    小鼠单克隆抗体[3A3] to Hsp70
  • 宿主

    Mouse
  • 特异性

    ab5439 detects several members of the heat shock protein 70 kDa (Hsp 70) gene family including Hsp 70, Hsc 70, p75, and following heat shock, Hsp 72 from yeast, Drosophila, fish, mouse, avian, amphibian and human samples.
  • 经测试应用

    适用于: ICC, IHC-P, WBmore details
  • 种属反应性

    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant fragment corresponding to Human Hsp70 aa 504-617 (C terminal).

  • 表位

    Epitope mapping with a panel of Hsp 70 deletion mutants suggests that the epitope recognized is located between amino acids 504-617 of human Hsp 70, a region that has been shown to be involved in stress-induced nucleolar localization.
  • 阳性对照

    • WB: HeLa, K562, Jurkat, 293T, A431, A549, F9, RAW264.7 and C6, PC-12 lysates. IHC-P: Human cerebrum and lung carcinoma, Mouse testis, Rat cerebrum tissues. ICC: HeLa and NIH/3T3, PC-12 cells.
  • 常规说明

    This product has switched from a hybridoma to recombinant production method on 08th March 2021.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • 纯度

    Protein A purified
  • Primary antibody说明

    The Hsp 70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human Hsp 70 family members include: Hsp 70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; Hsp 72, a 72 kDa protein that is induced exclusively under stress conditions; Hsc 70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or Hsp 75, a 75 kDa protein that is found within the mitochondria.
  • 克隆

    单克隆
  • 克隆编号

    3A3
  • 同种型

    IgG1
  • 研究领域

    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Heat Shock Proteins
    • Cancer
    • Tumor biomarkers
    • Other
    • Neuroscience
    • Processes

相关产品

  • Alternative Versions

    • Anti-Hsp70 antibody [3A3] - BSA and Azide free (ab252240)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant human Hsp70 protein (Active) (ab78434)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab5439于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC
1/50.
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB (3)
1/1000. Predicted molecular weight: 70-78 kDa.
说明
ICC
1/50.
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Predicted molecular weight: 70-78 kDa.

靶标

  • 相关性

    Function: In cooperation with other chaperones, the Hsp70 family stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Tissue specificity: HSPA1B is testis-specific.
  • 细胞定位

    Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • 数据库链接

    • Entrez Gene: 15511 Human
    • Entrez Gene: 3303 Human
    • Entrez Gene: 193740 Mouse
    • Entrez Gene: 3304 Mouse
    • Entrez Gene: 24472 Rat
    • Entrez Gene: 294254 Rat
    • Omim: 140550 Human
    • Omim: 603012 Human
    • SwissProt: P0DMV8 Human
    • SwissProt: P0DMV9 Human
    • SwissProt: P17879 Mouse
    • SwissProt: Q61696 Mouse
    • SwissProt: P0DMW0 Rat
    • SwissProt: P0DMW1 Rat
    see all
  • 别名

    • DnaK type molecular chaperone HSP70 1 antibody
    • Epididymis secretory protein Li 103 antibody
    • FLJ54303 antibody
    • FLJ54370 antibody
    • FLJ54392 antibody
    • FLJ54408 antibody
    • FLJ75127 antibody
    • Heat shock 70 kDa protein 1 antibody
    • Heat shock 70 kDa protein 1/2 antibody
    • Heat shock 70 kDa protein 1A antibody
    • Heat shock 70 kDa protein 1A/1B antibody
    • Heat shock 70kDa protein 1B antibody
    • Heat shock induced protein antibody
    • HEL S 103 antibody
    • HSP70 1 antibody
    • HSP70 1A antibody
    • HSP70 1B antibody
    • HSP70 2 antibody
    • HSP70-1 antibody
    • HSP70-1/HSP70-2 antibody
    • HSP70-1A antibody
    • HSP70-2 antibody
    • HSP70.1 antibody
    • HSP70.1/HSP70.2 antibody
    • HSP71_HUMAN antibody
    • HSP72 antibody
    • HSPA1 antibody
    • HSPA1A antibody
    • HSPA1B antibody
    see all

图片

  • Western blot - Anti-Hsp70 antibody [3A3] (ab5439)
    Western blot - Anti-Hsp70 antibody [3A3] (ab5439)
    All lanes : Anti-Hsp70 antibody [3A3] (ab5439) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
    Lane 4 : 293T (human embryonic kidney epithelial cell), whole cell lysate
    Lane 5 : A431 (human epidermoid carcinoma epithelial cell), whole cell lysate
    Lane 6 : A549 (human lung carcinoma epithelial cell), whole cell lysate
    Lane 7 : F9 (mouse embryonal carcinoma epithelial cell), whole cell lysate
    Lane 8 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
    Lane 9 : C6 (rat glial tumor glial cell), whole cell lysate
    Lane 10 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 70-78 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?


    Exposure time: 1 second


    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [3A3] (ab5439)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [3A3] (ab5439)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Hsp70 with ab5439 at 1/2000 (0.519 µg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human cerebrum. The section was incubated with ab5439 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins



  • Immunocytochemistry - Anti-Hsp70 antibody [3A3] (ab5439)
    Immunocytochemistry - Anti-Hsp70 antibody [3A3] (ab5439)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa cells labelling Hsp70 with ab5439 at 1/50 (20.76 µg/ml) dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and weak nuclear staining in HeLa cells ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [3A3] (ab5439)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [3A3] (ab5439)

    Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling Hsp70 with ab5439 at 1/2000 (0.519 µg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human lung carcinoma. The section was incubated with ab5439 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunocytochemistry - Anti-Hsp70 antibody [3A3] (ab5439)
    Immunocytochemistry - Anti-Hsp70 antibody [3A3] (ab5439)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling Hsp70 with ab5439 at 1/50 (20.76 µg/ml) dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and weak nuclear staining in NIH/3T3 cells ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [3A3] (ab5439)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [3A3] (ab5439)

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling Hsp70 with ab5439 at 1/2000 (0.519 µg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse testis. The section was incubated with ab5439 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunocytochemistry - Anti-Hsp70 antibody [3A3] (ab5439)
    Immunocytochemistry - Anti-Hsp70 antibody [3A3] (ab5439)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized PC-12 cells labelling Hsp70 with ab5439 at 1/50 (20.76 µg/ml) dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in PC-12 cells ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [3A3] (ab5439)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [3A3] (ab5439)

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Hsp70 with ab5439 at 1/2000 (0.519 µg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat cerebrum. The section was incubated with ab5439 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Anti-Hsp70 antibody [3A3] (ab5439)
    Anti-Hsp70 antibody [3A3] (ab5439)

实验方案

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (44)

发表研究结果有使用 ab5439?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab5439 被引用在 44 文献中.

  • Liu Y  et al. Selenogenome and AMPK signal insight into the protective effect of dietary selenium on chronic heat stress-induced hepatic metabolic disorder in growing pigs. J Anim Sci Biotechnol 12:68 (2021). PubMed: 34116728
  • Fukuta T  et al. Low level electricity increases the secretion of extracellular vesicles from cultured cells. Biochem Biophys Rep 21:100713 (2020). PubMed: 31828227
  • Kong F  et al. Cold Exposure-Induced Up-Regulation of Hsp70 Positively Regulates PEDV mRNA Synthesis and Protein Expression In Vitro. Pathogens 9:N/A (2020). PubMed: 32224931
  • Adriaenssens E  et al. BAG3 Pro209 mutants associated with myopathy and neuropathy relocate chaperones of the CASA-complex to aggresomes. Sci Rep 10:8755 (2020). PubMed: 32472079
  • Hu W  et al. Effects of transport stress on pathological injury and expression of main heat shock proteins in the caprine stomach. BMC Vet Res 16:347 (2020). PubMed: 32962700
View all Publications for this product

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1-9 of 9 Abreviews or Q&A

Western blot abreview for Anti-Hsp70 antibody [3A3]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (cell lysates in 25% Nupage LSD, 10% DTT, 65% milli)
Gel Running Conditions
Reduced Denaturing (12.5% Acrylamide gel)
Loading amount
35 µg
Specification
cell lysates in 25% Nupage LSD, 10% DTT, 65% milli
Blocking step
Rockland blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
Read More

Abcam user community

Verified customer

提交于 May 27 2015

Western blot abreview for Anti-Hsp70 [3A3] antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (8% acrylamide)
Sample
Pig Tissue lysate - other (Muscle sarcoplasmic extract)
Specification
Muscle sarcoplasmic extract
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Dr. Shannon Cruzen

Verified customer

提交于 Mar 27 2014

Western blot abreview for Anti-Hsp70 [3A3] antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (12% Tris Glycine)
Sample
Human Cell lysate - whole cell (U2OS and HeLa cells)
Specification
U2OS and HeLa cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

提交于 Jan 18 2011

Question

Inquiry: I am looking for anti-GM130 and anti-Hsp70 antibodies for use with MCF7 cell lysates and MCF7 cell exosomes. I see that you have ab5439 and ab52649 that have both been used successfully with MCF7 cells, however I want to use the antibodies for Western blotting, and not IHC as stated on the data sheets. Would these antibodies be suitble for Western Blotting? Or do you have alternative versions of the antibodies that would be suitable? Alternatively an Alix antibody to be used for western blots with the same cell line could replace the Hsp70 antibody if there is not one available. Thanks

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Abcam community

Verified customer

Asked on Jan 17 2013

Answer

Thank you for your enquiry.

I would like to reassure you that both these antibodies are covered by our 6 month guarantee for WB and for human samples.

For more details regarding our guarantee, please see the following page from our website:

https://www.abcam.com/index.html?pageconfig=abpromise

As far as we are aware, the antibodies have not been specifically tried in WB with MCF7 cells. However, this is still likely to work, as they have been tested in WB and human, and so this would still be covered by our guarantee.

Also, you can obtain rewards by submitting an Abreview about these product via the online product datasheets. We always encourage customers to send their results back to us, whether positive or negative, and we make all product information available to researchers.

To find out more about our Abreview system and the Abpoints rewards you can obtain, please see the following webpage:

https://www.abcam.com/abreviews

I hope this will be helpful. Thank you for your time and effort.

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Abcam Scientific Support

回复于 Jan 17 2013

Question

Q: Hi do you determine the protein concentration before IP?

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Abcam community

Verified customer

Asked on Nov 21 2011

Answer

Thank you for attending our webinar on Immunoprecipitation last week. We hope you enjoyed it and it was useful for you. We would also like to thank you for sending your question and apologize that we were not able to answer it in the Q&A session. The determination of the protein concentration before IP is not different from that for other techniques such as Western blot. Therefore, you could perform a Bradford assay, a Lowry assay or a BCA assay. Bovine serum albumin (BSA) is a frequently-used protein standard. Once you have determined the concentration of each sample, you can freeze them at -20°C or -80°C for later use or prepare for immunoprecipitation or for loading onto a gel. For the actual IP experiment, in a tube add 10-500 μg cell lysate plus the recommended amount of antibody. These amounts will be chosen depending on the abundance of the protein and the affinity of the antibody for the protein, typically in a pilot experiment where a fixed amount of protein is precipitated by increasing amounts of antibody. You can check the antibody datasheet for recommended antibody concentration. As a guideline we recommend: 1 – 5 μl polyclonal antiserum 1 μg affinity-purified polyclonal antibody 0.2 to 1 μl ascites fluid (monoclonal antibody) 20 to 100 μl culture supernatant (monoclonal antibody) I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Abcam Scientific Support

回复于 Nov 21 2011

Question

Thank you for your response.   Our customer has responded with the following;   Thanks for your response. We decide to order AB77179 (Anti-Entactin antibody), AB6314 (Anti-Cathepsin L antibody), AB38975 (Anti-TIMP2 antibody) and AB41684 (Anti-Cyclophilin A antibody) with testing discount. Could you please provide discount code to me. If you could kindly provide the codes soon that would be great.  Enjoy your day Kind Regards        

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Abcam community

Verified customer

Asked on Nov 08 2011

Answer

Thank you for your message and for confirming these details. I am very pleased to hear the customer would like to accept our offer and test these antibodies in hamster. ab41684 Discount code: #### ab38975 Discount code: #### ab6314 Discount code: #### ab77179 Discount code: #### Expirey date for all these codes: 7th March 2012 This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit the Abreviews for hamster samples and include the relevant code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount. If you have any further questions, please do not hesitate to contact us. We look forward to receiving the Abreviews and wish the customer luck with their research.

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Abcam Scientific Support

回复于 Nov 08 2011

Question

Hi,  I hope you are well. The customer has responded with the following;  I finally have time to find the protein sequence and get back to you. Could someone help me to check whether these three antibodies have testing discount (Entactin, Cathepsin B and L)? The comment is attached in the email. The sequence for Cathepsin B and L are also in the word document. Although I couldn’t find the sequence of Entactin, we still would like to try it. Thanks a lot. See attached a document showing the sequences.   AB77179 AB6314 AB16045   Is our customer eligible for the discount of these 3 antibodies?   I look forward to your response.     Kind Regards  

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Abcam community

Verified customer

Asked on Oct 31 2011

Answer

Thank you for your message which has been forwarded to me as Karin is currently away from the office. I have reviewed these three antibodies with respect to discount codes: ab77179 I am sorry I am also unable to check the alignment of the immunogen with the hamster sequence, because there is regrettably no hamster sequence available on the protein database sites. I would be pleased to make an exception and provide a discount code in this case anyway, to test in Hamster. ab6314 There is 73% Alignment of the human full length immunogen to the hamster sequence you have provided. This is a little low, but there may be some crossreactivity so I would be pleased to provide a discount. ab16045 There is 14% alignment of the hamster sequence provided with the immunogen. This is very low so the antibody will probably not detect in hamster, and so I am sorry we would be unable to provide a discount for this particular antibody to test in hamster. I hope this will be helpful to you. Please confirm if the customer would like to receive a code for testing ab6314 and ab77179 in hamster and I will be pleased to arrange this for you.

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Abcam Scientific Support

回复于 Oct 31 2011

Question

Dear Tech support, I have a researcher who is looking to test a number of antibodies against hamster tissue for western blot. Below are the antibodies of interest. As these antibodies have not been validated against hamster would a testing discount apply in this case?   ab77179 Anti-Entactin ab5439 Anti-Hsp70 ab6314 Anti-Cathepsin L ab16045 Anti-Cyclophilin B ab109708 Anti-TIMP2 ab41684 Anti-Cyclophilin A Could you please let me know if you could offer the testing discount or if there are any guarantees for the antibodies as hamster is not listed in the validated species. Hope to hear from you soon. Best regards,

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Abcam community

Verified customer

Asked on Oct 20 2011

Answer

Thank you for contacting us. If an antibody has not been tested with a particular species then a testing discount would apply. However, in order to check the likelihood of the antibody being reactive against the target the hamster protein sequence needs to be compared to that of the immunogen used to make sure that the most suitable antibody is chosen. Unfortunately I have been unable to find sequences for Entactin, Cathepsin L or Cyclophilin B for hamster so if you or your customer could help me with this that would be very useful. We already have several antibodies directed against Hsp70 which have been tested with hamster samples. ab47454 is a mouse monoclonal or ab79852 is a rabbit polyclonal. We also have several which are directly conjugated that may be of interest too. These can be found from the following link: https://www.abcam.com/index.html?pageconfig=searchresults&search=hsp70&pt=1&sk=app&sv=69&sn=WB&l=3&fViewMore=1 The Cyclophilin A antibody suggested (ab41684) is predicted to react and would therefore be suitable for a testing discount. However, with the TIMP2 antibody suggested (ab109708), the immunogen recognises the N-terminal sequence of the protein which differs significantly from that of the hamster protein. I would therefore recommend ab38975 as an alternative. This is a rabbit polyclonal directed against the C-terminal end which shares significant homology with Chinese hamster sequence (SwissProt Q60453) compared to the human (SwissProt P16035). I hope this information is helpful to you. If you'd like me to issue testing discounts for ab41684 or ab38975. Or you would like to stick with the original Hsp70 antibody recommended please let me know. Please do not hesitate to contact us if you need any more advice or information.

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Abcam Scientific Support

回复于 Oct 20 2011

Question

I'm looking for an antibody that specifically recognizes the stress inducible heat shock protein 70 (Hsp70) of Xenopus laevis. I have already tried a couple of antibodies from Stressgen, but they all recognize the constitutively expressed Xenopus hsc70 protein and not the inducible protein. Both proteins are very similar except for the C-terminal region (amino acids 520-640). I found two Abcam antibodies that should specifically recognize the C-terminus of Hsp70, namely Abcam 3A3 (ab5439) and Abcam 4G4 (ab5444), but the product information does not state that these antibodies also recognize the proper protein in Xenopus laevis. After my bad experience with the Stressgen antibodies, I do not wish to spend a lot of money on antibodies that recognize the wrong protein in Xenopus. Therefore, I would like to know if it is possible to get a test sample of the two Abcam antibodies. If one or both antibody(ies) recognize the inducible Hsp70 in Xenopus larvae and A6 cell lysates I would of course be happy to order a regular amount of antibody.

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Abcam community

Verified customer

Asked on Aug 25 2005

Answer

Thank you for your email. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Ab5439 reacts with Amphibians, Chicken, Drosophilia melanogaster, Fish, Human, S. cerevisiae, Mouse and Rat. Therefore, we will guarantee that the antibody will work with Xenopus samples in the following applications that ab5439 has been tested in: Western blot, Immunoprecipitation, Immunohistochemistry (Formalin-fixed paraffin-embedded sections), and Immunocytochemistry. Please note that ab5439 detects several members of the heat shock protein 70 kDa (Hsp 70) gene family including Hsp 70, Hsc 70, p75, and following heat shock, Hsp 72 from yeast, Drosophila, fish, mouse, avian, amphibian and human samples. As far as we are aware, cross reactivity with Xenopus has not yet been tested for use with ab5444 (so far it has only been tested for corss reactivity with human). Should you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview and in return we will award you 50 Abcam Points, which can be redeemed on a number of rewards (a further 100 points will be offered for an image). If you have any additional questions, please contact us again.

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Abcam Scientific Support

回复于 Aug 25 2005

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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