Application
Western blot
Sample
Rat Tissue lysate - whole (whole skin)
Loading amount
15 µg
Specification
whole skin
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5%
Other product details
Dilution
1/2000
Incubation time
12 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti Mouse
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Detection
Detection method
nbt/bcip
Exposure
30 minute(s) and 0 second(s)
Bands
Specific: 75 kDa
Positive control
Ran 4 various samples (two of each type) on 10% gel and 2 of the same type give a deep band at 75kDa. Primary was incubated overnight at 4 degrees celcius.
Additional data
Additional Notes
Antibody buffer for primary and secondary was 1.5% milk in TBS-T. Used 10% gel and a PVDF Membrane was use as blot paper.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jan 31 2007