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Signal Transduction Protein Trafficking Chaperones Heat Shock Proteins

Anti-Hsp27 (phospho S15)抗体(ab5581)

  • Datasheet
Submit a review Q&A (1)References (2)

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Western blot - Anti-Hsp27 (phospho S15) antibody (ab5581)
  • Immunoprecipitation - Anti-Hsp27 (phospho S15) antibody (ab5581)
  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 (phospho S15) antibody (ab5581)

Key features and details

  • Rabbit polyclonal to Hsp27 (phospho S15)
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Rat, Rabbit, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-Hsp27 (phospho S15)抗体
    参阅全部 Hsp27 一抗
  • 描述

    兔多克隆抗体to Hsp27 (phospho S15)
  • 宿主

    Rabbit
  • 特异性

    ab5581 detects phosphorylated heat shock protein 27(hsp27) from rat and Human tissues.
  • 经测试应用

    适用于: WB, ICC/IF, IPmore details
  • 种属反应性

    与反应: Rat, Rabbit, Human
    预测可用于: Cow, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to Human Hsp27 aa 10-21 (phospho S15).
    Sequence:

    LLRGPSWDPFRC


    (Peptide available as ab41772)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Heat Shock Proteins
    • Cancer
    • Tumor biomarkers
    • Other
    • Cardiovascular
    • Atherosclerosis
    • Ischemia / Reperfusion
    • Cardiovascular
    • Heart
    • Contractility
    • Contractile Proteins
    • Actins

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Hsp27 (phospho S82) protein (ab113185)

应用

Our Abpromise guarantee covers the use of ab5581 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/1000. Predicted molecular weight: 23 kDa.
ICC/IF 1/50 - 1/200.
IP Use at an assay dependent concentration.

3 μg

靶标

  • 功能

    Involved in stress resistance and actin organization.
  • 组织特异性

    Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.
  • 疾病相关

    Defects in HSPB1 are the cause of Charcot-Marie-Tooth disease type 2F (CMT2F) [MIM:606595]. CMT2F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. CMT2F onset is between 15 and 25 years with muscle weakness and atrophy usually beginning in feet and legs (peroneal distribution). Upper limb involvement occurs later. CMT2F inheritance is autosomal dominant.
    Defects in HSPB1 are a cause of distal hereditary motor neuronopathy type 2B (HMN2B) [MIM:608634]. Distal hereditary motor neuronopathies constitute a heterogeneous group of neuromuscular disorders caused by selective impairment of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs.
  • 序列相似性

    Belongs to the small heat shock protein (HSP20) family.
  • 翻译后修饰

    Phosphorylated in MCF-7 cells on exposure to protein kinase C activators and heat shock.
  • 细胞定位

    Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
  • Target information above from: UniProt accession P04792 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 516099 Cow
    • Entrez Gene: 3315 Human
    • Entrez Gene: 493184 Pig
    • Entrez Gene: 24471 Rat
    • Omim: 602195 Human
    • SwissProt: Q3T149 Cow
    • SwissProt: P04792 Human
    • SwissProt: Q5S1U1 Pig
    • SwissProt: P42930 Rat
    • Unigene: 520973 Human
    • Unigene: 3841 Rat
    see all
  • 别名

    • Heat shock 27kDa protein antibody
    • 28 kDa heat shock protein antibody
    • CMT2F antibody
    • DKFZp586P1322 antibody
    • epididymis secretory protein Li 102 antibody
    • Estrogen regulated 24 kDa protein antibody
    • Estrogen-regulated 24 kDa protein antibody
    • Heat shock 25kDa protein 1 antibody
    • Heat shock 27 kDa protein antibody
    • Heat shock 27kD protein 1 antibody
    • Heat shock 27kDa protein 1 antibody
    • Heat shock 28kDa protein 1 antibody
    • Heat Shock Protein 27 antibody
    • Heat shock protein beta 1 antibody
    • Heat shock protein beta-1 antibody
    • heat shock protein family B (small) member 1 antibody
    • HEL-S-102 antibody
    • HMN2B antibody
    • HS.76067 antibody
    • Hsp 25 antibody
    • HSP 27 antibody
    • Hsp 28 antibody
    • Hsp B1 antibody
    • Hsp25 antibody
    • HSP27 antibody
    • Hsp28 antibody
    • HspB1 antibody
    • HSPB1_HUMAN antibody
    • SRP27 antibody
    • Stress responsive protein 27 antibody
    • Stress-responsive protein 27 antibody
    see all

图片

  • Western blot - Anti-Hsp27 (phospho S15) antibody (ab5581)
    Western blot - Anti-Hsp27 (phospho S15) antibody (ab5581)
    All lanes : Anti-Hsp27 (phospho S15) antibody (ab5581) at 1/500 dilution

    Lane 1 : HeLa cell lysate - untreated
    Lane 2 : HeLa cell lysate - treated with 10nM Anisomycin

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/20000 dilution

    Predicted band size: 23 kDa

  • Immunoprecipitation - Anti-Hsp27 (phospho S15) antibody (ab5581)
    Immunoprecipitation - Anti-Hsp27 (phospho S15) antibody (ab5581)

    Immunoprecipitation of Hsp27 (phospho S15) was performed on HeLa cells treated with 10uM Anisomysin for 30 minutes. Antigen-antibody complexes were formed by incubating 500ug of whole cell lysate with 3ug of ab5581 overnight on a rocking platform at 4°C. The immune complexes were captured on 50ul Protein A/G Agarose, washed extensively, and eluted with Lane Marker Reducing Sample Buffer. Samples were then resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with ab5581 at 1:500 overnight rotating at 4°C, washed with TBST, and probed with Clean-Blot IP Detection Reagent at 1/1000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.

  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 (phospho S15) antibody (ab5581)
    Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 (phospho S15) antibody (ab5581)

    Immunocytochemistry/Immunofluorescence analysis of Hsp27 (phospho S15) (green) in HeLa cells either untreated (left) or treated with 10uM Anisomysin (right) for 30 minutes. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% blocker BSA for 15 minutes at room temperature. Cells were incubated with ab5581 at 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (1:400) for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye. 20X magnification.

实验方案

  • Immunoprecipitation protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

    • Datasheet
  • 文献 (2)

    发表研究结果有使用 ab5581?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab5581 被引用在 2 文献中.

    • Li KC  et al. Reduced expression of HSP27 following HAD-B treatment is associated with Her2 downregulation in NIH:OVCAR-3 human ovarian cancer cells. Mol Med Rep 12:3787-94 (2015). PubMed: 26044344
    • Williams KL  et al. Hsp27 and axonal growth in adult sensory neurons in vitro. BMC Neurosci 6:24 (2005). PubMed: 15819993

    客户评价及客户问答

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    Question

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    Abcam community

    Verified customer

    Asked on Feb 08 2006

    Answer

    Thank you for your enquiry. Unfortunately I do not have specific images of ICC and IHC that I can upload to our datasheets. However, the following publication used this antibody to beautiful affect. Williams KL, Rahimtula M, Mearow KM. Hsp27 and axonal growth in adult sensory neurons in vitro. BMC Neurosci. 2005 6(1):24. PMID: 15819993 The publication can be accessed at the following link: http://www.biomedcentral.com/content/pdf/1471-2202-6-24.pdf I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    回复于 Feb 09 2006

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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