Key features and details
- Mouse monoclonal [G3.1] to Hsp27
- Suitable for: Flow Cyt, ICC/IF, IHC-P, WB
- Reacts with: Rat, Sheep, Human, Non human primates
- Isotype: IgG1
参阅全部 Hsp27 一抗
描述小鼠单克隆抗体[G3.1] to Hsp27
经测试应用适用于: Flow Cyt, ICC/IF, IHC-P, WBmore details
种属反应性与反应: Rat, Sheep, Human, Non human primates
预测可用于: Chicken, Cow, Pig
Full length native protein (purified) corresponding to Human Hsp27. (Partially purified human HSP27)
- WB: Hela whole cell lysate; ICC/IF: MCF7 cells; Flow Cyt: MCF7 cells; IHC-P: Human breast carcinoma and human prostate carcinoma.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA
Concentration information loading...
纯度Tissue culture supernatant
Our Abpromise guarantee covers the use of ab2790 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use a concentration of 1 - 2 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use a concentration of 1 - 2 µg/ml.|
|IHC-P||Use a concentration of 1 - 2 µg/ml.
Perform heat mediated antigen retrieval using 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C followed by cooling at room temperature for 20 minutes.
Incubate with primary for 30 minutes at room temperature.
|WB||Use a concentration of 0.25 - 0.5 µg/ml.|
功能Involved in stress resistance and actin organization.
组织特异性Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.
疾病相关Defects in HSPB1 are the cause of Charcot-Marie-Tooth disease type 2F (CMT2F) [MIM:606595]. CMT2F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. CMT2F onset is between 15 and 25 years with muscle weakness and atrophy usually beginning in feet and legs (peroneal distribution). Upper limb involvement occurs later. CMT2F inheritance is autosomal dominant.
Defects in HSPB1 are a cause of distal hereditary motor neuronopathy type 2B (HMN2B) [MIM:608634]. Distal hereditary motor neuronopathies constitute a heterogeneous group of neuromuscular disorders caused by selective impairment of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs.
序列相似性Belongs to the small heat shock protein (HSP20) family.
翻译后修饰Phosphorylated in MCF-7 cells on exposure to protein kinase C activators and heat shock.
细胞定位Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
- Information by UniProt
- Heat shock 27kDa protein antibody
- 28 kDa heat shock protein antibody
- CMT2F antibody
Anti-Hsp27 antibody [G3.1] (ab2790) + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Observed band size: 27 kDa why is the actual band size different from the predicted?
Immunohistochemistry of paraffin embedded human breast carcinoma with ab2790 labeling Hsp27 at 0.5μg/ml.
Immunohistochemistry of paraffin embedded human prostate carcinoma with ab2790 labeling Hsp27 at 0.5μg/ml.
Immunocytochemistry/immunofluorescence analysis of paraformaldehyde fixed MCF7 (human breast adenocarcinoma cell line) cells labelling Hsp27 with ab2790 at 2 µg/mL. Goat Anti-Mouse IgG was used as the secondary antibody (green). Nuclear DNA labelled red.
Anti-Hsp27 antibody [G3.1] (ab2790) at 1/1000 dilution + Mouse whole brain tissue lysate. at 10 µg
An HRP-conjugated Goat polyclonal. at 1/10000 dilution
Developed using the ECL technique.
Observed band size: 24 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
Blocking Step: 5% Milk for 1 hour at 25°C.
Gel Running Conditions: Reduced, Denaturing Bis-tris 4-12%
Overlay histogram showing HeLa cells (ab150035) stained with ab2790 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum (ab7481) / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2790, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
ab2790 被引用在 32 文献中.
- Drexler R et al. Significance of unphosphorylated and phosphorylated heat shock protein 27 as a prognostic biomarker in pancreatic ductal adenocarcinoma. J Cancer Res Clin Oncol N/A:N/A (2020). IHC-P ; Human . PubMed: 32200459
- Terra LF et al. Heat shock protein B1 is a key mediator of prolactin-induced beta-cell cytoprotection against oxidative stress. Free Radic Biol Med 134:394-405 (2019). PubMed: 30699366
- Huang CY et al. Silencing Heat Shock Protein 27 Inhibits the Progression and Metastasis of Colorectal Cancer (CRC) by Maintaining the Stability of Stromal Interaction Molecule 1 (STIM1) Proteins. Cells 7:N/A (2018). PubMed: 30544747
- Sun P et al. Foot-and-mouth disease virus capsid protein VP2 activates the cellular EIF2S1-ATF4 pathway and induces autophagy via HSPB1. Autophagy 14:336-346 (2018). PubMed: 29166823
- San Gil R et al. Using bicistronic constructs to evaluate the chaperone activities of heat shock proteins in cells. Sci Rep 7:2387 (2017). PubMed: 28539657