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Signal Transduction Protein Trafficking Chaperones Heat Shock Proteins

Anti-Hsp27抗体(ab5579)

  • Datasheet
Reviews (1)Q&A (2)References (16)

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Western blot - Anti-Hsp27 antibody (ab5579)
  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 antibody (ab5579)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody (ab5579)
  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 antibody (ab5579)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody (ab5579)
  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 antibody (ab5579)
  • Western blot - Anti-Hsp27 antibody (ab5579)
  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 antibody (ab5579)

Key features and details

  • Rabbit polyclonal to Hsp27
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human, African green monkey
  • Isotype: IgG

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概述

  • 产品名称

    Anti-Hsp27抗体
    参阅全部 Hsp27 一抗
  • 描述

    兔多克隆抗体to Hsp27
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性

    与反应: Mouse, Rat, Human, African green monkey
  • 免疫原

    Synthetic peptide corresponding to Human Hsp27 aa 10-21.
    Sequence:

    LLRGPSWDPFRC


    (Peptide available as ab39789)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照

    • WB: HEK-293T, HeLa, K562, A431, HepG2, COS-7, NIH/3T3, MCF7, MDA-MB-231, PC3, DU 145, LNCaP, HT1080 whole cell lysate. IHC-P: Human skeletal muscle and breast carcinoma tissue. ICC: HeLa, MCF-7 and C6 cells.
  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    Antigen affinity chromatography.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Heat Shock Proteins
    • Cancer
    • Tumor biomarkers
    • Other
    • Cardiovascular
    • Atherosclerosis
    • Ischemia / Reperfusion
    • Cardiovascular
    • Heart
    • Contractility
    • Contractile Proteins
    • Actins

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Hsp27 (phospho S82) protein (ab113185)

应用

Our Abpromise guarantee covers the use of ab5579 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/2000. Detects a band of approximately 27 kDa.
ICC/IF 1/50.
IHC-P Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.

靶标

  • 功能

    Involved in stress resistance and actin organization.
  • 组织特异性

    Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.
  • 疾病相关

    Defects in HSPB1 are the cause of Charcot-Marie-Tooth disease type 2F (CMT2F) [MIM:606595]. CMT2F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. CMT2F onset is between 15 and 25 years with muscle weakness and atrophy usually beginning in feet and legs (peroneal distribution). Upper limb involvement occurs later. CMT2F inheritance is autosomal dominant.
    Defects in HSPB1 are a cause of distal hereditary motor neuronopathy type 2B (HMN2B) [MIM:608634]. Distal hereditary motor neuronopathies constitute a heterogeneous group of neuromuscular disorders caused by selective impairment of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs.
  • 序列相似性

    Belongs to the small heat shock protein (HSP20) family.
  • 翻译后修饰

    Phosphorylated in MCF-7 cells on exposure to protein kinase C activators and heat shock.
  • 细胞定位

    Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
  • Target information above from: UniProt accession P04792 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 3315 Human
    • Entrez Gene: 15507 Mouse
    • Entrez Gene: 24471 Rat
    • Omim: 602195 Human
    • SwissProt: P04792 Human
    • SwissProt: P14602 Mouse
    • SwissProt: P42930 Rat
    • Unigene: 520973 Human
    • Unigene: 13849 Mouse
    • Unigene: 3841 Rat
    see all
  • 别名

    • Heat shock 27kDa protein antibody
    • 28 kDa heat shock protein antibody
    • CMT2F antibody
    • DKFZp586P1322 antibody
    • epididymis secretory protein Li 102 antibody
    • Estrogen regulated 24 kDa protein antibody
    • Estrogen-regulated 24 kDa protein antibody
    • Heat shock 25kDa protein 1 antibody
    • Heat shock 27 kDa protein antibody
    • Heat shock 27kD protein 1 antibody
    • Heat shock 27kDa protein 1 antibody
    • Heat shock 28kDa protein 1 antibody
    • Heat Shock Protein 27 antibody
    • Heat shock protein beta 1 antibody
    • Heat shock protein beta-1 antibody
    • heat shock protein family B (small) member 1 antibody
    • HEL-S-102 antibody
    • HMN2B antibody
    • HS.76067 antibody
    • Hsp 25 antibody
    • HSP 27 antibody
    • Hsp 28 antibody
    • Hsp B1 antibody
    • Hsp25 antibody
    • HSP27 antibody
    • Hsp28 antibody
    • HspB1 antibody
    • HSPB1_HUMAN antibody
    • SRP27 antibody
    • Stress responsive protein 27 antibody
    • Stress-responsive protein 27 antibody
    see all

图片

  • Western blot - Anti-Hsp27 antibody (ab5579)
    Western blot - Anti-Hsp27 antibody (ab5579)
    All lanes : Anti-Hsp27 antibody (ab5579) at 1/2000 dilution

    Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
    Lane 2 : MDA-MB-231 (human breast adenocarcinoma cell line) whole cell lysate
    Lane 3 : PC3 (human prostate adenocarcinoma cell line) whole cell lysate
    Lane 4 : DU 145 (human prostate carcinoma cell line) whole cell lysate
    Lane 5 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 6 : LNCaP (human prostate cancer cell line) whole cell lysate
    Lane 7 : HT1080 (human fibrosarcoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution
  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 antibody (ab5579)
    Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 antibody (ab5579)

    Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Hsp27 (green) with ab5579 at 1/200 dilution, followed by DyLight 488-conjugated secondary antibody. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. 60X magnification. Right - negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody (ab5579)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody (ab5579)

    Immunohistochemical analysis of both normal and cancer biopsies of deparaffinized human skeletal muscle tissue labeling Hsp27 with ab5579 at 1/20 dilution or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

    To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature.

  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 antibody (ab5579)
    Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 antibody (ab5579)

    Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma cell line) cells labeling Hsp27 (green) with ab5579 at 1/200 dilution, followed by DyLight 488-conjugated secondary antibody. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. 60X magnification. Right - negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody (ab5579)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody (ab5579)

    Immunohistochemical analysis of both normal and cancer biopsies of deparaffinized human breast carcinoma tissue labeling Hsp27 with ab5579 at 1/100 dilution or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

    To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. 

  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 antibody (ab5579)
    Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 antibody (ab5579)

    Immunofluorescence analysis of C6 (Rat glial tumor cell line) cells labeling Hsp27 (green) with ab5579 at 1/100 dilution, followed by DyLight 488-conjugated secondary antibody. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. 60X magnification. Right - negative control.

  • Western blot - Anti-Hsp27 antibody (ab5579)
    Western blot - Anti-Hsp27 antibody (ab5579)
    All lanes : Anti-Hsp27 antibody (ab5579) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : K562 (human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate
    Lane 4 : A431 (human epidermoid carcinoma cell line) whole cell lysate
    Lane 5 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 6 : COS-7 (african green monkey kidney fibroblast-like cell line) whole cell lysate
    Lane 7 : NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG-HRP secondary antibody at 1/20000 dilution
  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 antibody (ab5579)
    Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 antibody (ab5579)This image is courtesy of Michael Manicini, Ph.D.

    HeLa (human epithelial cell line from cervix adenocarcinoma) cells were fixed with 4% formaldehyde in PEM buffer. The coverslip was incubated in blocking buffer of 5% powdered milk in TBS-T plus 0.02% sodium azide for 1 hour at room temperature. Blocking buffer was removed and primary antibody was added at a dilution of 1/250 and incubated overnight at 4 degrees celsius. The coverslips were then washed 4-5 times with blocking buffer for 5 minutes. Secondary antibody, goat anti-rabbit Alexa 594 (ab150080), was added at a dilution of 1/1000 and incubated at room temperature for one hour. From this point on coverslips were covered with foil to protect them from light. They were washed 5 times with TBS-T and then one time with PEM, for 5 minutes each wash. The coverslips were fixed 10-30 minutes in 4% formaldehyde in PEM buffer, then washed 3 times with PEM buffer for 5 minutes. 0.1M ammonium chloride in PEM buffer was added for 10 minutes to quench auto-florescence, and then slips were washed 2 times for 5 minutes in PEM followed by 3 washes for 5 minute

实验方案

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

    • Datasheet
  • 文献 (16)

    发表研究结果有使用 ab5579?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab5579 被引用在 16 文献中.

    • Liu QH  et al. Reduced expression of annexin A1 promotes gemcitabine and 5-fluorouracil drug resistance of human pancreatic cancer. Invest New Drugs 38:350-359 (2020). PubMed: 31124054
    • Xue T & Chun-Li A Role of Pneumocystis jirovecii infection in chronic obstructive pulmonary disease progression in an immunosuppressed rat Pneumocystis pneumonia model. Exp Ther Med 19:3133-3142 (2020). PubMed: 32256801
    • Chen Y  et al. O-GlcNAcylated c-Jun antagonizes ferroptosis via inhibiting GSH synthesis in liver cancer. Cell Signal 63:109384 (2019). PubMed: 31394193
    • Yamashita M  et al. Uhrf1 is indispensable for normal limb growth by regulating chondrocyte differentiation through specific gene expression. Development 145:N/A (2018). PubMed: 29180567
    • Cox D & Ecroyd H The small heat shock proteins aB-crystallin (HSPB5) and Hsp27 (HSPB1) inhibit the intracellular aggregation of a-synuclein. Cell Stress Chaperones 22:589-600 (2017). PubMed: 28337642
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-3 of 3 Abreviews or Q&A

    Question

    Phone call: customer kindly asked if theyc an use ab5579 for therapeutic purposes?

    Read More

    Abcam community

    Verified customer

    Asked on Jan 09 2012

    Answer

    Thank you for contacting us. Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE" I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    回复于 Jan 09 2012

    Western blot abreview for Anti-Hsp27 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Spleen)
    Specification
    Spleen
    Blocking step
    Other as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: N/A
    Read More

    Abcam user community

    Verified customer

    提交于 Oct 18 2005

    Question

    I have recieved them, on Friday. The Rabbit polyclonal to Hsp27 (ab5579) has a shortfall in it, i spun the tube down prior to opening, and have aliquoted into other tubes but i only had 80ul, not 100ul as i was expecting.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 10 2004

    Answer

    Thank you for your enquiry. We are going to send you a free of charge replacement vial very shortly. For your information, the new order number is: 36004. We apologize for any inconvenience caused and thank you for bringing this matter to our attention.

    Read More

    Abcam Scientific Support

    回复于 Mar 11 2004

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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