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Signal Transduction Protein Trafficking Chaperones Heat Shock Proteins

Anti-Hsp27抗体(ab1426)

  • Datasheet
  • SDS
Reviews (1)Q&A (2)References (9)

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Western blot - Anti-Hsp27 antibody (ab1426)

    Key features and details

    • Rabbit polyclonal to Hsp27
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-Hsp27抗体
      参阅全部 Hsp27 一抗
    • 描述

      兔多克隆抗体to Hsp27
    • 宿主

      Rabbit
    • 特异性

      The antibody detects a 27 kDa protein, corresponding to the apparent molecular mass of Hsp27 on SDS-PAGE immunoblots, in samples from human, monkey, dog (weakly) and pig (weakly) origins.
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • 免疫原

      Recombinant human Hsp27 protein.

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • 存储溶液

      pH: 7.20
      Preservative: 0.02% Sodium azide
      Constituents: 50% Glycerol, 1% BSA
    • Concentration information loading...
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Signal Transduction
      • Protein Trafficking
      • Chaperones
      • Heat Shock Proteins
      • Cancer
      • Tumor biomarkers
      • Other
      • Cardiovascular
      • Atherosclerosis
      • Ischemia / Reperfusion
      • Cardiovascular
      • Heart
      • Contractility
      • Contractile Proteins
      • Actins

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human Hsp27 (phospho S82) protein (ab113185)

    应用

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab1426 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    应用 Species
    WB
    Human
    应用 Ab评论 说明
    WB (1)
    Use a concentration of 1 - 2 µg/ml.
    说明
    WB
    Use a concentration of 1 - 2 µg/ml.

    靶标

    • 功能

      Involved in stress resistance and actin organization.
    • 组织特异性

      Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.
    • 疾病相关

      Defects in HSPB1 are the cause of Charcot-Marie-Tooth disease type 2F (CMT2F) [MIM:606595]. CMT2F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. CMT2F onset is between 15 and 25 years with muscle weakness and atrophy usually beginning in feet and legs (peroneal distribution). Upper limb involvement occurs later. CMT2F inheritance is autosomal dominant.
      Defects in HSPB1 are a cause of distal hereditary motor neuronopathy type 2B (HMN2B) [MIM:608634]. Distal hereditary motor neuronopathies constitute a heterogeneous group of neuromuscular disorders caused by selective impairment of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs.
    • 序列相似性

      Belongs to the small heat shock protein (HSP20) family.
    • 翻译后修饰

      Phosphorylated in MCF-7 cells on exposure to protein kinase C activators and heat shock.
    • 细胞定位

      Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
    • Target information above from: UniProt accession P04792 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 3315 Human
      • Omim: 602195 Human
      • SwissProt: P04792 Human
      • Unigene: 520973 Human
      • 别名

        • Heat shock 27kDa protein antibody
        • 28 kDa heat shock protein antibody
        • CMT2F antibody
        • DKFZp586P1322 antibody
        • epididymis secretory protein Li 102 antibody
        • Estrogen regulated 24 kDa protein antibody
        • Estrogen-regulated 24 kDa protein antibody
        • Heat shock 25kDa protein 1 antibody
        • Heat shock 27 kDa protein antibody
        • Heat shock 27kD protein 1 antibody
        • Heat shock 27kDa protein 1 antibody
        • Heat shock 28kDa protein 1 antibody
        • Heat Shock Protein 27 antibody
        • Heat shock protein beta 1 antibody
        • Heat shock protein beta-1 antibody
        • heat shock protein family B (small) member 1 antibody
        • HEL-S-102 antibody
        • HMN2B antibody
        • HS.76067 antibody
        • Hsp 25 antibody
        • HSP 27 antibody
        • Hsp 28 antibody
        • Hsp B1 antibody
        • Hsp25 antibody
        • HSP27 antibody
        • Hsp28 antibody
        • HspB1 antibody
        • HSPB1_HUMAN antibody
        • SRP27 antibody
        • Stress responsive protein 27 antibody
        • Stress-responsive protein 27 antibody
        see all

      图片

      • Western blot - Anti-Hsp27 antibody (ab1426)
        Western blot - Anti-Hsp27 antibody (ab1426)
        All lanes : Anti-Hsp27 antibody (ab1426) at 2 µg/ml

        Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
        Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
        Lane 3 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

        Observed band size: 25,27 kDa why is the actual band size different from the predicted?

      实验方案

      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols
      • Western blot protocols

      Click here to view the general protocols

      数据表及文件

      • SDS download

      • Datasheet download

        Download

      文献 (9)

      发表研究结果有使用 ab1426?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab1426 被引用在 9 文献中.

      • Cheng YC  et al. Knocking down of heat-shock protein 27 directs differentiation of functional glutamatergic neurons from placenta-derived multipotent cells. Sci Rep 6:30314 (2016). WB ; Human . PubMed: 27444754
      • Monari E  et al. Analysis of protein expression in periodontal pocket tissue: a preliminary study. Proteome Sci 13:33 (2015). WB ; Human . PubMed: 26719749
      • Li KC  et al. Reduced expression of HSP27 following HAD-B treatment is associated with Her2 downregulation in NIH:OVCAR-3 human ovarian cancer cells. Mol Med Rep 12:3787-94 (2015). PubMed: 26044344
      • Yang T  et al. Epigenetic inactivation of EFEMP1 is associated with tumor suppressive function in endometrial carcinoma. PLoS One 8:e67458 (2013). WB, IHC . PubMed: 23840707
      • Sakai A  et al. Identification of phosphorylated serine-15 and -82 residues of HSPB1 in 5-fluorouracil-resistant colorectal cancer cells by proteomics. J Proteomics 75:806-18 (2012). WB ; Human . PubMed: 21989268
      • Bruinsma IB  et al. Small heat shock proteins induce a cerebral inflammatory reaction. J Neurosci 31:11992-2000 (2011). ELISA, IHC-Fr ; Human . PubMed: 21849559
      • Wilhelmus MM  et al. Small heat shock proteins associated with cerebral amyloid angiopathy of hereditary cerebral hemorrhage with amyloidosis (Dutch type) induce interleukin-6 secretion. Neurobiol Aging 30:229-40 (2009). IHC-Fr ; Human . PubMed: 17629591
      • Wilhelmus MM  et al. Specific association of small heat shock proteins with the pathological hallmarks of Alzheimer's disease brains. Neuropathol Appl Neurobiol 32:119-30 (2006). WB, IHC-Fr ; Human . PubMed: 16599941
      • Timofeeva OA  et al. Serine-phosphorylated STAT1 is a prosurvival factor in Wilms' tumor pathogenesis. Oncogene 25:7555-64 (2006). WB ; Human . PubMed: 16799645

      客户评价及客户问答

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      1-3 of 3 Abreviews or Q&A

      Western blot abreview for Anti-Hsp27 antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Rat Cell lysate - whole cell (Pulmonary microvascular endothelial cells)
      Gel Running Conditions
      Non-reduced Denaturing
      Loading amount
      100 µg
      Specification
      Pulmonary microvascular endothelial cells
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      提交于 Aug 17 2015

      Question

      We need to be certain that this antibody only reacts to human HSP27, and not murine HSP27. We are working with a transgenic mouse that expresses human 27 in particular tissues.

      Read More

      Abcam community

      Verified customer

      Asked on Apr 18 2012

      Answer

      Thank you for contacting us regarding finding a product which will only react to human HSP27, and not murine HSP27.

      I do want to apologize for the delay in getting this information to you. Two of our products will fit your needs very well.

      ab1426: This product is very specific for human and monkey samples and weakly interacts with dog and pig samples. There is no reactivity with mouse samples.
      https://www.abcam.com/Hsp27-antibody-ab1426.html
      ab115856: I provided a BLAST against Human Hsp 27 and also did an alignment with the mouse version of this marker.  There is around 80%+ homology but it must not be significant enough to get cross reactivity with mouse based on our data.  The WB we have on the web for this one which clearly shows no bands for either mouse protein or mouse cell line.
      https://www.abcam.com/Hsp27-antibody-ab115856.html

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      回复于 Apr 18 2012

      Question

      What is the actual immunogen for this antibody. It is not explicitly stated whether this was made against full-length human HSP27, etc.

      Read More

      Abcam community

      Verified customer

      Asked on Nov 15 2005

      Answer

      Recombinant human Hsp27 protein. If you have any additional questions, please contact us again.

      Read More

      Abcam Scientific Support

      回复于 Nov 16 2005

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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