Key features and details
- Rabbit polyclonal to Hsc70
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Hamster, Cow, Human, Pig, Monkey
- Isotype: IgG
参阅全部 Hsc70 一抗
特异性The antibody detects a 73 kDa protein, corresponding to the apparent molecular weight of Hsc70 on SDS-PAGE immunoblots. No reactivity with inducible Hsp70 is detected.
经测试应用适用于: WB, IHC-Pmore details
种属反应性与反应: Mouse, Rat, Hamster, Cow, Human, Pig, Monkey
The 70 kDa heat shock cognate protein, Hsc70, is closely related to Hsp70 and is part of the Hsp70 family. The family contains a number of highly-related protein isoforms ranging in size from 66 kDa to 78 kDa. These proteins include both cognate members which are found within major intracellular compartments and highly inducible isoforms which appear to be predominantly cytoplasmic or nuclear in distribution. Members of the Hsp70 family are molecular chaperones which are involved in many cellular functions such as protein folding, transport, maturation and degradation and they exert their function in an AATP-dependent manner.
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.03% Proclin 300
Constituents: PBS, 30% Glycerol, 0.5% BSA, 0.015% EDTA
Concentration information loading...
Primary antibody说明The 70 kDa heat shock cognate protein, Hsc70, is closely related to Hsp70 and is part of the Hsp70 family. The family contains a number of highly-related protein isoforms ranging in size from 66 kDa to 78 kDa. These proteins include both cognate members which are found within major intracellular compartments and highly inducible isoforms which appear to be predominantly cytoplasmic or nuclear in distribution. Members of the Hsp70 family are molecular chaperones which are involved in many cellular functions such as protein folding, transport, maturation and degradation and they exert their function in an AATP-dependent manner.
Our Abpromise guarantee covers the use of ab1427 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 71 kDa.|
|IHC-P||Use at an assay dependent concentration.|
功能Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones.
序列相似性Belongs to the heat shock protein 70 family.
结构域The N-terminal 1-386 residues constitute the ATPase domain, while residues 387-646 form the peptide-binding domain.
翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
细胞定位Cytoplasm. Melanosome. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- 2410008N15Rik antibody
- Constitutive heat shock protein 70 antibody
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Ab1427 staining human normal testis tissue. Staining is localised to cytoplasmic and nuclear compartments.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplif
All lanes : Anti-Hsc70 antibody (ab1427) at 1/2500 dilution
Lane 1 : U2OS cell lysate
Lane 2 : HeLa cell lysate
All lanes : HRP-conjugated Goat polyclonal to Rabbit at 1/2000 dilution
Performed under reducing conditions.
Predicted band size: 71 kDa
Observed band size: 71 kDa
Exposure time: 2 minutes
ab1427 被引用在 11 文献中.
- Cao Y et al. Augmentation of vaccine-induced humoral and cellular immunity by a physical radiofrequency adjuvant. Nat Commun 9:3695 (2018). PubMed: 30209303
- Xu CY et al. DJ-1 Inhibits a-Synuclein Aggregation by Regulating Chaperone-Mediated Autophagy. Front Aging Neurosci 9:308 (2017). PubMed: 29021755
- Soleilhavoup C et al. Proteomes of the Female Genital Tract During the Oestrous Cycle. Mol Cell Proteomics 15:93-108 (2016). PubMed: 26518761
- Gao L et al. Oxidation of survival factor MEF2D in neuronal death and Parkinson's disease. Antioxid Redox Signal 20:2936-48 (2014). PubMed: 24219011
- Dermentzaki G et al. Loss of ß-glucocerebrosidase activity does not affect alpha-synuclein levels or lysosomal function in neuronal cells. PLoS One 8:e60674 (2013). WB ; Human . PubMed: 23580063