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Signal Transduction Protein Trafficking Chaperones Heat Shock Proteins

Anti-Hsc70抗体(ab1427)

  • Datasheet
  • SDS
Reviews (2)Q&A (4)References (11)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsc70 antibody (ab1427)

    Key features and details

    • Rabbit polyclonal to Hsc70
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-Hsc70抗体
      参阅全部 Hsc70 一抗
    • 描述

      兔多克隆抗体to Hsc70
    • 宿主

      Rabbit
    • 特异性

      The antibody detects a 73 kDa protein, corresponding to the apparent molecular weight of Hsc70 on SDS-PAGE immunoblots. No reactivity with inducible Hsp70 is detected.
    • Tested Applications & Species

      Application Species
      IHC-P
      Human
      See all applications and species data
    • 免疫原

      Synthetic peptide:

      EKLQGKINDEDKQKI

      , corresponding to amino acids 555-570 of Human Hsc70
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 常规说明


      The 70 kDa heat shock cognate protein, Hsc70, is closely related to Hsp70 and is part of the Hsp70 family. The family contains a number of highly-related protein isoforms ranging in size from 66 kDa to 78 kDa. These proteins include both cognate members which are found within major intracellular compartments and highly inducible isoforms which appear to be predominantly cytoplasmic or nuclear in distribution. Members of the Hsp70 family are molecular chaperones which are involved in many cellular functions such as protein folding, transport, maturation and degradation and they exert their function in an AATP-dependent manner.

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • 存储溶液

      Preservative: 0.03% Proclin 300
      Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA, 0.015% EDTA
    • Concentration information loading...
    • Primary antibody说明

      The 70 kDa heat shock cognate protein, Hsc70, is closely related to Hsp70 and is part of the Hsp70 family. The family contains a number of highly-related protein isoforms ranging in size from 66 kDa to 78 kDa. These proteins include both cognate members which are found within major intracellular compartments and highly inducible isoforms which appear to be predominantly cytoplasmic or nuclear in distribution. Members of the Hsp70 family are molecular chaperones which are involved in many cellular functions such as protein folding, transport, maturation and degradation and they exert their function in an AATP-dependent manner.
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Signal Transduction
      • Protein Trafficking
      • Chaperones
      • Heat Shock Proteins

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant human Hsc70 protein (ab78431)

    应用

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab1427 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    应用 Species
    IHC-P
    Human
    应用 Ab评论 说明
    IHC-P
    Use at an assay dependent concentration.
    说明
    IHC-P
    Use at an assay dependent concentration.

    靶标

    • 功能

      Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones.
    • 组织特异性

      Ubiquitous.
    • 序列相似性

      Belongs to the heat shock protein 70 family.
    • 结构域

      The N-terminal 1-386 residues constitute the ATPase domain, while residues 387-646 form the peptide-binding domain.
    • 翻译后修饰

      Phosphorylated upon DNA damage, probably by ATM or ATR.
      ISGylated.
    • 细胞定位

      Cytoplasm. Melanosome. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Target information above from: UniProt accession P11142 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 3312 Human
      • Omim: 600816 Human
      • SwissProt: P11142 Human
      • Unigene: 180414 Human
      • 别名

        • 2410008N15Rik antibody
        • Constitutive heat shock protein 70 antibody
        • Epididymis luminal protein 33 antibody
        • Epididymis secretory sperm binding protein Li 72p antibody
        • Heat shock 70 kDa protein 8 antibody
        • Heat shock 70kD protein 10 antibody
        • Heat shock 70kD protein 8 antibody
        • Heat shock 70kDa protein 8 antibody
        • Heat shock cognate 71 kDa protein antibody
        • Heat shock cognate protein 54 antibody
        • Heat shock cognate protein 71 kDa antibody
        • Heat shock protein 8 antibody
        • Heat shock protein A8 antibody
        • Heat shock protein family A (Hsp70) member 8 antibody
        • Heat-shock70-KD protein 10, formerly antibody
        • HEL 33 antibody
        • HEL S 72p antibody
        • HSC54 antibody
        • HSC71 antibody
        • Hsc73 antibody
        • HSP71 antibody
        • HSP73 antibody
        • HSP7C_HUMAN antibody
        • HSPA10 antibody
        • HSPA8 antibody
        • LAP1 antibody
        • Lipopolysaccharide associated protein 1 antibody
        • LPS associated protein 1 antibody
        • LPS associated protein antibody
        • MGC102007 antibody
        • MGC106514 antibody
        • MGC114311 antibody
        • MGC118485 antibody
        • MGC131511 antibody
        • MGC29929 antibody
        • N-myristoyltransferase inhibitor protein 71 antibody
        • NIP71 antibody
        see all

      图片

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsc70 antibody (ab1427)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsc70 antibody (ab1427)
        Ab1427 staining human normal testis tissue. Staining is localised to cytoplasmic and nuclear compartments.
        Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
        Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplif

      实验方案

      • Immunohistochemistry protocols

      Click here to view the general protocols

      数据表及文件

      • SDS download

      • Datasheet download

        Download

      文献 (11)

      发表研究结果有使用 ab1427?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab1427 被引用在 11 文献中.

      • Cao Y  et al. Augmentation of vaccine-induced humoral and cellular immunity by a physical radiofrequency adjuvant. Nat Commun 9:3695 (2018). PubMed: 30209303
      • Xu CY  et al. DJ-1 Inhibits a-Synuclein Aggregation by Regulating Chaperone-Mediated Autophagy. Front Aging Neurosci 9:308 (2017). PubMed: 29021755
      • Soleilhavoup C  et al. Proteomes of the Female Genital Tract During the Oestrous Cycle. Mol Cell Proteomics 15:93-108 (2016). PubMed: 26518761
      • Gao L  et al. Oxidation of survival factor MEF2D in neuronal death and Parkinson's disease. Antioxid Redox Signal 20:2936-48 (2014). PubMed: 24219011
      • Dermentzaki G  et al. Loss of ß-glucocerebrosidase activity does not affect alpha-synuclein levels or lysosomal function in neuronal cells. PLoS One 8:e60674 (2013). WB ; Human . PubMed: 23580063
      View all Publications for this product

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      1-6 of 6 Abreviews or Q&A

      Western blot abreview for Anti-Hsc70 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Loading amount
      10 µg
      Gel Running Conditions
      Reduced Denaturing
      Sample
      Sheep Serum (Seminal Plasma)
      Specification
      Seminal Plasma
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
      Read More

      Mr. Guillaume Tsikis

      Verified customer

      提交于 Jul 15 2013

      Western blot abreview for Anti-Hsc70 antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (U2OS and HeLa cells)
      Loading amount
      30 µg
      Specification
      U2OS and HeLa cells
      Gel Running Conditions
      Reduced Denaturing (12% Tris Glycine)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      提交于 Jan 18 2011

      Question

      BATCH NUMBER 202129 ORDER NUMBER 182689 DESCRIPTION OF THE PROBLEM no band SAMPLE 3T3 cell lysate PRIMARY ANTIBODY ab1427 1µg/ml in blocker for 1-2 hours, wash 3x5min in PBS DETECTION METHOD Fluorescence on a kodak IS4000MM using proper excitation and emission filters for the secondary antibodies POSITIVE AND NEGATIVE CONTROLS USED all samples were positive controls. ANTIBODY STORAGE CONDITIONS -20 degrees SAMPLE PREPARATION reduced and boiled 5min @95 degrees AMOUNT OF PROTEIN LOADED 20µg ELECTROPHORESIS/GEL CONDITIONS reducing 4-12% bis-tris gel 125V for 2 hours TRANSFER AND BLOCKING CONDITIONS overnight at 30V 5% milk + PBS blocker SECONDARY ANTIBODY Goat anti-rabbit Qdot 705 and Goat Anti-rabbit Alexa 750 at 1:1000 in blocker for 1 hour, wash 3x5min in PBS HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? Changed the cell lysate to A431 cell lysate, no change

      Read More

      Abcam community

      Verified customer

      Asked on Nov 08 2006

      Answer

      Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I have read your technical questionnaire and I have a few comments. You are largely following an approach that I would recommend; using 3T3 mouse cells, a cell type that has been routinely used in the literature as a positive control for the detection of Hsc70 by western blotting. Can you tell me whether you have performed a ponceau red detection of the transfered protein or confirmed the integrity of the lysates using an alternative antibody that targets a "housekeeping" protein such as GAPDH. In the lab at Abcam we frequently find that a change to the blocking agent and/or antibody diluent produces more specific sensitive results. On this occasion I would like to recommend that you try applying the antibody using 3% BSA as a blocking agent, replacing milk. I would also like to recommend the use of TBST (as opposed to PBS) as the antibody diluent and wash buffer. Should a change to the blocking agent not improve your results please get back in touch with me and I will be happy to offer you a replacement vial (provided that the purchase was made within the past 90 days). I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      回复于 Nov 09 2006

      Question

      Is this antibody a purified antibody? If so, to what extent has it been purified?

      Read More

      Abcam community

      Verified customer

      Asked on Aug 24 2006

      Answer

      Dear Dr. Lloyd, Thank you for your enquiry. Regarding ab1427 (Hsc70 antibody), this product has been Protein A/G purified.

      Read More

      Abcam Scientific Support

      回复于 Aug 24 2006

      Question

      Customer is using this antibody in WB with recombinant protein but is not seeing a signal. The primary was used at 1-10 ug/ml and the signal detected was extremely weak. Lot# 132030.

      Read More

      Abcam community

      Verified customer

      Asked on Dec 02 2005

      Answer

      Thank you for your phone call and I'm sorry to hear that you are experiencing difficulty with ab1427. We have not received any other complaints regarding this antibody and it may be that the vial you received was damaged. I would be happy to send you a free of charge replacement vial or can have a credit note or refund issued to you. Please let me know how you would like to proceed and I look forward to hearing from you.

      Read More

      Abcam Scientific Support

      回复于 Dec 05 2005

      Question

      To what part of the Hsc70 molecule (N-end, C-end, middle, sequence) is the antibody directed?

      Read More

      Abcam community

      Verified customer

      Asked on Oct 07 2005

      Answer

      Thank you for your enquiry regarding ab1427. Synthetic peptide surrounding amino acid 559 of human Hsc70 was used to raise this antibody.

      Read More

      Abcam Scientific Support

      回复于 Oct 11 2005

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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