HRP Anti-VSV-G tag抗体(ab3556)

Submit a review Q&A (2)References (3)

Key features and details

  • HRP Rabbit polyclonal to VSV-G tag
  • Suitable for: WB, ELISA
  • Reacts with: Species independent
  • Conjugation: HRP
  • Isotype: IgG

概述

  • 产品名称

    HRP Anti-VSV-G tag抗体
    参阅全部 VSV-G tag 一抗

  • 描述

    HRP兔多克隆抗体to VSV-G tag

  • 宿主

    Rabbit

  • 偶联物

    HRP

  • 经测试应用

    适用于: WB, ELISAmore details

  • 种属反应性

    与反应: Species independent

  • 免疫原

    Synthetic peptide corresponding to VSV-G tag conjugated to keyhole limpet haemocyanin.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C.

  • 存储溶液

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA
  • Concentration information loading...

  • 纯度

    Immunogen affinity purified

  • 纯化说明

    Antibodies were immunoaffinity purified using the peptide immobilized on a solid phase.

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab3556于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/5000.
ELISA
1/5000 - 1/20000.
说明
WB
1/1000 - 1/5000.
ELISA
1/5000 - 1/20000.

靶标

  • 相关性

    Vesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The glycoprotein (VSV-g) contains a domain in its extracellular membrane proximal stem that appears to be needed for efficient VSV budding.

  • 别名

    • Vesicular stomatitis virus glycoprotein tag antibody
    • VSV epitope tag antibody
    • VSV tag antibody
    • VSV-G epitope tag antibody
    • VSVG antibody
    • vsvg tag antibody
    see all

文献 (3)

发表研究结果有使用 ab3556？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab3556 被引用在 3 文献中.

  • Masuda Y  et al. Regulation of HLTF-mediated PCNA polyubiquitination by RFC and PCNA monoubiquitination levels determines choice of damage tolerance pathway. Nucleic Acids Res 46:11340-11356 (2018). PubMed: 30335157
  • Bellon A  et al. VEGFR2 (KDR/Flk1) signaling mediates axon growth in response to semaphorin 3E in the developing brain. Neuron 66:205-19 (2010). WB . PubMed: 20434998
  • Thyrell L  et al. Interferon alpha-induced apoptosis in tumor cells is mediated through the phosphoinositide 3-kinase/mammalian target of rapamycin signaling pathway. J Biol Chem 279:24152-62 (2004). PubMed: 15056668

客户评价及客户问答

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Question

Hello,



I am looking for an antibody for detecting whether my cells are expressing VSV-G (Western blot). I have found several ab on-line, but do you also have cell lysate as a positive control?



Best regards

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Answer

Thank you for your enquiry and your interest in our products.

We have a few antibodies which detect VSV-G and are suitable for Western applications. Some of them are unconjugated so it is essential to apply a compatible secondary antibody for detection:

ab1874: https://www.abcam.com/vsv-g-tag-antibody-ab1874.html

ab27026: https://www.abcam.com/vsv-g-tag-antibody-ab27026.html

ab50549: https://www.abcam.com/vsv-g-tag-antibody-p5d4-ab50549.html

Some others are HRP-labelled so can be applied without any secondary antibodies:

ab3556: https://www.abcam.com/vsv-g-tag-antibody-hrp-ab3556.html

ab49610: https://www.abcam.com/vsv-g-tag-antibody-p5d4-hrp-ab49610.html

Currently, we do not sel lpositive control cell lysate for VSV-G. As long as the cells you are planning to use express the tag sequence: YTDIEMNRLGK; these antibodies should work fine.

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Question

WESTERN BLOT TECHNICAL ENQUIRY - Wed 11 Feb 2004, 10:42hrs ========================================================== ANTIBODY CODE ab3556 BATCH NUMBER 37614 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM Non-specific band I immunoprecipitate 2 VSV-G tagged proteins using a mouse monoclonal antibody and want to detect my immunoprecipitatd proteins with the VSV G hrp rabbit polyclonal antibody. I have no problem to detect the first one (so I know that my immunoprecipitation is OK), but the problem is that at the size of my second VSV-G tagged proteins there are plenty of non specific bands, so I detect a band even in my controls where I don't express this second protein. So I cannot prove that I have immunoprecipitated my second protein too. I tried different dilutions of the antibody but this does not solve my problem. Can you indicate me your Western Blot conditions? Furthermore, this antibody reveals the heavy and light chains. I wanted to know if this antody is known to cross-react ? Thank you Nathalie Franck SAMPLE HeLa cell extract incubated with a VSV-G monoclonal antibody (which works well) PRIMARY ANTIBODY VSV-g hrp tested at different dilutions 1/1000-1/5000 SECONDARY ANTIBODY none DETECTION METHOD super signal (Pierce) POSITIVE AND NEGATIVE CONTROLS USED Negative control: Lysates transfected with a control protein also VSV-G tagged. I detect this protein (no contaminating bands at this size (100 kDa). But at the size of my protein of interest, I detect a contaminating band in my negative control ANTIBODY STORAGE CONDITIONS Storage: 4°C SAMPLE PREPARATION Buffer containing 150 mM NaCL, Tris 10 mM, NP40 0,5%, Triton-X-100 1%, EDTA, EGTA, Na3VO4, PMSF, protease inhibitor (Roche) AMOUNT OF PROTEIN LOADED 15-20 µg of total cell lysate or 10 µl of immunoprecipitation product (I use 500 µg for one immunoprecipitation; beads are respuspended in 25 µl of SB2X ELECTROPHORESIS/GEL CONDITIONS SDS-PAGE 12,5% TRANSFER AND BLOCKING CONDITIONS Transfer 45 minutes 300 mA. I block my membran with 5% dry non fat milk in Tris buffered saline containing 0,1% Tween. HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? Dilution of the antibody and incubation time (overnight or 1h at ambiant temperature

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Answer

Thank you very much for your patience. I am working with the originator of ab3556 to help figure out what may be going on with this particular antibody. We have a few more questions however that will help us out. 1) What exactly does your negative control consist of? 2) How are you isolating the monoclonal antibody/VSV-g tagged protein? 3) Do you isolate by eluting the protein or centrifuging? 4) How do you know it is recognizing the heavy and light chains? 5)Have you tried any inhibition studies?

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