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Tags & Cell Markers Epitope Tags Myc Tag

HRP Anti-Myc tag抗体(ab1326)

  • Datasheet
  • SDS
Reviews (3)Q&A (3)References (15)

Product price, shipping and contact information

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送货和订单信息

Abpromise

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Key features and details

  • HRP Rabbit polyclonal to Myc tag
  • Suitable for: ELISA, WB, ICC
  • Conjugation: HRP
  • Isotype: IgG

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概述

  • 产品名称

    HRP Anti-Myc tag抗体
    参阅全部 Myc tag 一抗
  • 描述

    HRP兔多克隆抗体to Myc tag
  • 宿主

    Rabbit
  • 偶联物

    HRP
  • 特异性

    In a direct ELISA against 1 ug of C-Myc peptide coupled to BSA, conjugate diluted to 1/20,000 produced a 1.0 OD in a 15 minute reaction with TMB as substrate.
  • 经测试应用

    适用于: ELISA, WB, ICCmore details
  • 免疫原

    Synthetic peptide: EQKLISEEDL conjugated to KLH.

  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C.
  • 存储溶液

    pH: 7.00
    Preservative: 0.002% Thimerosal (merthiolate)
    Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA
  • Concentration information loading...
  • 纯度

    Affinity purified
  • 纯化说明

    Antibodies were immunoaffinity purified using the peptide conjugated to a solid phase support, and conjugated to horseradish peroxidase.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Tags & Cell Markers
    • Epitope Tags
    • Myc Tag

相关产品

  • Immunizing Peptide (Blocking)

    • Human c-Myc peptide (ab13837)
  • Related Products

    • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
    • TMB ELISA Substrate (High Sensitivity) (ab171523)
    • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
    • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
    • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
    • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
    • 450 nm Stop Solution for TMB Substrate (ab171529)
    • 650 nm Stop Solution for TMB Substrate (ab171531)
    • Immunoassay Blocking Buffer (ab171534)
    • Immunoassay Blocking (BSA Free) (ab171535)

应用

Our Abpromise guarantee covers the use of ab1326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/5000 - 1/20000.
WB 1/1000 - 1/5000. Can be blocked with Human c-Myc peptide (ab13837).
ICC 1/200 - 1/500.

靶标

  • 相关性

    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
  • 细胞定位

    Nuclear
  • 别名

    • c-myc tag antibody
    • Myc Epitope Tag antibody

实验方案

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

数据表及文件

    • Datasheet
    • SDS
  • 文献 (15)

    发表研究结果有使用 ab1326?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab1326 被引用在 15 文献中.

    • Lee S  et al. Absence of Cytosolic 2-Cys Prx Subtypes I and II Exacerbates TNF-a-Induced Apoptosis via Different Routes. Cell Rep 26:2194-2211.e6 (2019). PubMed: 30784599
    • Wild P  et al. Network Rewiring of Homologous Recombination Enzymes during Mitotic Proliferation and Meiosis. Mol Cell 75:859-874.e4 (2019). PubMed: 31351878
    • Xing J  et al. TRIM29 Negatively Regulates the Type I IFN Production in Response to RNA Virus. J Immunol 201:183-192 (2018). PubMed: 29769269
    • Compte M  et al. A tumor-targeted trimeric 4-1BB-agonistic antibody induces potent anti-tumor immunity without systemic toxicity. Nat Commun 9:4809 (2018). PubMed: 30442944
    • Kim JH  et al. WNK1 kinase is essential for insulin-stimulated GLUT4 trafficking in skeletal muscle. FEBS Open Bio 8:1866-1874 (2018). PubMed: 30410865
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-6 of 6 Abreviews or Q&A

    Western blot abreview for Anti-Myc tag antibody (HRP)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    10 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Saccharomyces cerevisiae Cell lysate - whole cell (S. cerevisiae cell lysate)
    Specification
    S. cerevisiae cell lysate
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    提交于 Mar 04 2014

    Question

    I have used this antibody and it is not working well for me in WB. I would like a refund.

    Read More

    Abcam community

    Verified customer

    Asked on Jun 14 2012

    Answer

    Thank you for contacting us.

    I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.

    Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.


    I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

    Read More

    Abcam Scientific Support

    回复于 Jun 14 2012

    Question

    Hello, is ab1326 the HRP-conjugated version of the ab9106 or of other myc antibodies? I don't quite fully understand the difference between the various primary rabbit polyclonal myc antibodies. Thanks, and happy new year!

    Read More

    Abcam community

    Verified customer

    Asked on Jan 05 2012

    Answer

    Thank you for contacting Abcam regarding our Anti-myc antibodies. I have confirmed that yes, ab1326 is the conjugated version of ab9106. I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

    Read More

    Abcam Scientific Support

    回复于 Jan 05 2012

    Western blot abreview for Anti-Myc tag antibody (HRP)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Escherichia coli Recombinant protein (BL21(DE3))
    Loading amount
    0.1 µg
    Specification
    BL21(DE3)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jun 09 2010

    Western blot abreview for Anti-Myc tag antibody (HRP)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Escherichia coli Recombinant protein (Expressed in DH10B cell)
    Loading amount
    0.5 µg
    Specification
    Expressed in DH10B cell
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    提交于 Mar 17 2010

    Question

    Sorry to bug you, but I have not received the vial of ab1326 yet. Is it possible for someone to check into it?

    Read More

    Abcam community

    Verified customer

    Asked on May 13 2004

    Answer

    No bother at all! Since I hadn't heard back about my last email, the vial was never shipped. I will have a vial, free of charge, shipped out to you straight away. You will receive it at the latest tomorrow. For your record it is order# 41583. Let me know if you have any more questions or concerns.

    Read More

    Abcam Scientific Support

    回复于 May 13 2004

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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