HRP Anti-Myc tag抗体(ab1326)
Key features and details
- HRP Rabbit polyclonal to Myc tag
- Suitable for: ELISA, WB, ICC
- Conjugation: HRP
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
HRP Anti-Myc tag抗体
参阅全部 Myc tag 一抗 -
描述
HRP兔多克隆抗体to Myc tag -
宿主
Rabbit -
偶联物
HRP -
特异性
In a direct ELISA against 1 ug of C-Myc peptide coupled to BSA, conjugate diluted to 1/20,000 produced a 1.0 OD in a 15 minute reaction with TMB as substrate. -
经测试应用
适用于: ELISA, WB, ICCmore details -
免疫原
Synthetic peptide corresponding to Myc tag conjugated to keyhole limpet haemocyanin.
(Peptide available asab13837) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.00
Preservative: 0.002% Thimerosal (merthiolate)
Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
Antibodies were immunoaffinity purified using the peptide conjugated to a solid phase support, and conjugated to horseradish peroxidase. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Immunizing Peptide (Blocking)
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Related Products
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
1/5000 - 1/20000.
|
|
| WB | (3) |
1/1000 - 1/5000. Can be blocked with Human c-Myc peptide (ab13837).
|
| ICC |
1/200 - 1/500.
|
| 说明 |
|---|
|
ELISA
1/5000 - 1/20000. |
|
WB
1/1000 - 1/5000. Can be blocked with Human c-Myc peptide (ab13837). |
|
ICC
1/200 - 1/500. |
靶标
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相关性
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. -
细胞定位
Nuclear -
别名
- c-myc tag antibody
- Myc Epitope Tag antibody
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (19)
ab1326 被引用在 19 文献中.
- Xing J et al. Identification of poly(ADP-ribose) polymerase 9 (PARP9) as a noncanonical sensor for RNA virus in dendritic cells. Nat Commun 12:2681 (2021). PubMed: 33976210
- Fan XJ et al. NONO phase separation enhances DNA damage repair by accelerating nuclear EGFR-induced DNA-PK activation. Am J Cancer Res 11:2838-2852 (2021). PubMed: 34249431
- Grigaitis R et al. Phosphorylation of the RecQ Helicase Sgs1/BLM Controls Its DNA Unwinding Activity during Meiosis and Mitosis. Dev Cell 53:706-723.e5 (2020). PubMed: 32504558
- Zhang A et al. EphA2 phosphorylates NLRP3 and inhibits inflammasomes in airway epithelial cells. EMBO Rep 21:e49666 (2020). PubMed: 32352641
- Lee S et al. Absence of Cytosolic 2-Cys Prx Subtypes I and II Exacerbates TNF-a-Induced Apoptosis via Different Routes. Cell Rep 26:2194-2211.e6 (2019). PubMed: 30784599