重组HRP Anti-Lysozyme抗体[EPR2994(2)] (ab197705)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR2994(2)] to Lysozyme
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
- Conjugation: HRP
Related conjugates and formulations
概述
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产品名称
HRP Anti-Lysozyme抗体[EPR2994(2)]
参阅全部 Lysozyme 一抗 -
描述
HRP兔单克隆抗体[EPR2994(2)] to Lysozyme -
宿主
Rabbit -
偶联物
HRP -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse不与反应: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: THP-1, HepG2, HL-60 whole cell lysate and human spleen tissue lysate. IHC-P: normal human tonsil tissue sections.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR2994(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab197705于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Detects a band of approximately 16 kDa (predicted molecular weight: 17 kDa).
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IHC-P |
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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WB
1/1000. Detects a band of approximately 16 kDa (predicted molecular weight: 17 kDa). |
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. -
疾病相关
Amyloidosis 8 -
序列相似性
Belongs to the glycosyl hydrolase 22 family. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 4069 Human
- Entrez Gene: 17105 Mouse
- Entrez Gene: 17110 Mouse
- Omim: 153450 Human
- SwissProt: P61626 Human
- SwissProt: P08905 Mouse
- Unigene: 524579 Human
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别名
- 1 4 beta N acetylmuramidase C antibody
- 1 antibody
- 4-beta-N-acetylmuramidase C antibody
see all
图片
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All lanes : HRP Anti-Lysozyme antibody [EPR2994(2)] (ab197705) at 1/1000 dilution
Lane 1 : Wild-type THP-1 cell lysate
Lane 2 : LYZ knockout THP-1 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 17 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutesab197705 was shown to react with Lysozyme in wild-type THP-1 cells in western blot. Loss of signal was observed when LYZ knockout sample was used. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab197705 overnight at 4 °C at a 1 in 1000 dilution. Blots were developed with Optiblot ECL reagent (ab133456) and imaged.
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IHC image of lysozyme staining in a section of formalin-fixed paraffin-embedded normal human tonsil*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab197705, 1/100 dilution, for 15 mins at room temperature.
DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre -
All lanes : HRP Anti-Lysozyme antibody [EPR2994(2)] (ab197705) at 1/1000 dilution
Lane 1 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate
Lane 2 : Spleen (Human) Tissue Lysate - adult normal tissue
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 17 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab197705 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
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