Application
Western blot
Sample
Human Recombinant protein (E coli)
Loading amount
2 µg
Specification
E coli
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: PBS/0.1% Tween
Secondary antibody
Secondary antibody
None used
Detection
Detection method
ECL+
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 16 kDa
Positive control
Recombinant IFN-alfa-2a purchased from BioVision
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Sep 13 2011