重组HRP Anti-Integrin alpha 2抗体[EPR17338] - C-terminal (ab209944)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR17338] to Integrin alpha 2 - C-terminal
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Rat, Human
- Conjugation: HRP
Related conjugates and formulations
概述
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产品名称
HRP Anti-Integrin alpha 2抗体[EPR17338] - C-terminal
参阅全部 Integrin alpha 2 一抗 -
描述
HRP兔单克隆抗体[EPR17338] to Integrin alpha 2 - C-terminal -
宿主
Rabbit -
偶联物
HRP -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Rat, Human
预测可用于: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: A549, T-47D, C6 and wild-type HAP1 whole cell lysates and human fetal brain tissue lysates. IHC-P: Normal human colon tissue sections.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 1% BSA, 30% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR17338 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab181548)
- Alexa Fluor® 488 Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab208770)
- Alexa Fluor® 647 Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab209766)
- Alexa Fluor® 594 Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab210655)
- Anti-Integrin alpha 2 antibody [EPR17338] - Low endotoxin, Azide free (ab222377)
- PE Anti-Integrin alpha 2 antibody [EPR17338] (ab225284)
- Anti-Integrin alpha 2 antibody [EPR17338] - BSA and Azide free (ab271936)
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab209944于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/5000. Detects a band of approximately 170 kDa (predicted molecular weight: 129 kDa).
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说明 |
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IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/5000. Detects a band of approximately 170 kDa (predicted molecular weight: 129 kDa). |
靶标
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功能
Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. -
序列相似性
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
结构域
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3673 Human
- Entrez Gene: 16398 Mouse
- Entrez Gene: 170921 Rat
- Omim: 192974 Human
- SwissProt: P17301 Human
- SwissProt: Q62469 Mouse
- Unigene: 482077 Human
- Unigene: 5007 Mouse
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别名
- BR antibody
- CD 49b antibody
- CD49 antigen like family member B antibody
see all
图片
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IHC image of Integrin alpha 2 staining in a section of formalin-fixed paraffin-embedded normal human colon*, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab209944, 1/50 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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All lanes : HRP Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab209944) at 1/5000 dilution
Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate at 10 µg
Lane 2 : T-47D (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg
Lane 3 : C6 (Rat glioma cell line) Whole Cell Lysate at 10 µg
Lane 4 : Brain (Human) Tissue Lysate - fetal normal tissue at 10 µg
Lane 5 : Wild-type HAP1 cell lysate at 20 µg
Lane 6 : Integrin alpha 2 knockout HAP1 cell lysate at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 129 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutesThis blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab209944 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
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