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Can you tell me what is the concentration/ratio of HRP conjugation to antibody?
Asked on Jan 13 2010
Thank you for your enquiry. Unfortunately, the concentration/ratio of HRP conjugation to antibody was not determined. However, I can inform you that the antibodies were conjugated to Sigma Horseradish Peroxidase, Type VI (P 8375) by modification of the periodate method. (Wilsom, M.B., Nakane, P.K., “Immunofluorescense and Related Staining Techniques“, Elsevier/North Holland Biomedical Press, Amsterdam, 215 (1978)). I am sorry I can be of more help in this occasion but if there is anything else that I can assist you with, please do not hesitate to contact me.
回复于 Jan 13 2010