HRP Anti-GST抗体(ab3416)
Key features and details
- HRP Rabbit polyclonal to GST
- Suitable for: WB, ELISA
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG
概述
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产品名称
HRP Anti-GST抗体
参阅全部 GST 一抗 -
描述
HRP兔多克隆抗体to GST -
宿主
Rabbit -
偶联物
HRP -
经测试应用
适用于: WB, ELISAmore details -
种属反应性
与反应: Species independent -
免疫原
Rabbits were immunized with Glutathione-S-transferase (GST) from Schistosoma japonicum.
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常规说明
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.00
Constituents: 3% Sodium deoxycholate, 3% Triton-X-100, 0.3% Tris HCl, 15% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Immunoaffinity purified using GST immobilized on a solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Recombinant Protein
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应用
Our Abpromise guarantee covers the use of ab3416 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/1000 - 1/5000. | |
| ELISA | 1/10000 - 1/25000. |
靶标
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相关性
GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins. -
别名
- Glutathione S Transferase antibody
- Glutathione S-transferase class-mu 26 kDa isozyme antibody
- GST antibody
see all
图片
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ELISA - HRP Anti-GST antibody (ab3416)This image is courtesy of an anonymous abreview.
Graph of ELISA measuring levels of bound GST-tagged mosquito proteins. 5g of target protein was coated onto an ELISA plate and incubated overnight at 4°C. The plate was washed and GST-tagged mosquito proteins were incubated for an hour at RT. The proteins were washed off, GST-HRP antibody (ab3416) was diluted 1/5000 in 1%BSA in PBS and added for 30 min at RT. The plate was washed and TMB substrate was added for 20 min at RT. Stop solution was added and the O.D. of the wells read at 450 nm.
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ELISA - HRP Anti-GST antibody (ab3416)This image is courtesy of an anonymous abreview.Graph of ELISA measuring levels of bound GST-tagged mosquito proteins. 5g of target protein was coated onto an ELISA plate and incubated overnight at 4°C. The plate was washed and GST-tagged mosquito proteins were incubated for an hour at RT. The proteins were washed off, GST-HRP antibody (ab3416) was diluted 1/5000 in 1%BSA in PBS and added for 30 min at RT. The plate was washed and TMB substrate was added for 20 min at RT. Stop solution was added and the O.D. of the wells read at 450 nm.
文献 (23)
ab3416 被引用在 23 文献中.
- Zyner KG et al. Genetic interactions of G-quadruplexes in humans. Elife 8:N/A (2019). PubMed: 31287417
- Liu K et al. PI31 Is an Adaptor Protein for Proteasome Transport in Axons and Required for Synaptic Development. Dev Cell 50:509-524.e10 (2019). PubMed: 31327739
- Liu H et al. Surface Loops in a Single SH2 Domain Are Capable of Encoding the Spectrum of Specificity of the SH2 Family. Mol Cell Proteomics 18:372-382 (2019). PubMed: 30482845
- Misra R et al. Tuning the Mycobacterium tuberculosis Alternative Sigma Factor SigF through the Multidomain Regulator Rv1364c and Osmosensory Kinase Protein Kinase D. J Bacteriol 201:N/A (2019). PubMed: 30642988
- McClurg UL et al. Human ex vivo prostate tissue model system identifies ING3 as an oncoprotein. Br J Cancer 118:713-726 (2018). PubMed: 29381681
