HRP Anti-Digoxigenin抗体[HRP.21H8] (ab6212)

Reviews (4)Q&A (13)References (18)
Immunohistochemistry (Frozen sections) - HRP Anti-Digoxigenin antibody [HRP.21H8] (ab6212)

    Key features and details

    • HRP Mouse monoclonal [HRP.21H8] to Digoxigenin
    • Suitable for: WB, ELISA, IHC-P, IHC-Fr
    • Reacts with: Species independent
    • Conjugation: HRP
    • Isotype: IgG1

    概述

    • 产品名称

      HRP Anti-Digoxigenin抗体[HRP.21H8]
      参阅全部 Digoxigenin 一抗

    • 描述

      HRP小鼠单克隆抗体[HRP.21H8] to Digoxigenin

    • 宿主

      Mouse

    • 偶联物

      HRP

    • 经测试应用

      适用于: WB, ELISA, IHC-P, IHC-Frmore details

    • 种属反应性

      与反应: Species independent

    • 免疫原

      Chemical/ Small Molecule conjugated to bovine serum albumin.

    • 常规说明

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Store at +4°C.

    • 存储溶液

      pH: 7.40
      Preservative: 0.01% Thimerosal (merthiolate)
      Constituents: PBS, 50% Glycerol, 1% BSA
    • Concentration information loading...

    • 纯度

      Protein A purified

    • 克隆

      单克隆

    • 克隆编号

      HRP.21H8

    • 骨髓瘤

      unknown

    • 同种型

      IgG1

    • 轻链类型

      kappa

    • 研究领域

    应用

    Our Abpromise guarantee covers the use of ab6212 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    WB 1/500 - 1/5000.
    ELISA 1/1000 - 1/10000.
    IHC-P 1/200 - 1/2000. Use in combination with tyramide signal amplification.
    IHC-Fr 1/200 - 1/2000. 1/200 - 1/2000. Use in combination with tyramide signal amplification.

    靶标

    • 相关性

      Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea and Digitalis lanata. Digoxigenin is chemically closely related to Digoxin, the cardiac glycoside used for the treatment of various heart diseases. The term 'genin' at the end of Digoxigenin, refers to only the aglycone portion (without the sugar) part of the molecule,thus Digoxigenin is the steroid component of Digoxin, - minus the (digitose) sugar residues. DIG can be covalently added to proteins or nucleic acids which makes it very useful in diverse applications.

    • 别名

      • 1672-46-4 antibody
      • BRN 0096479 antibody
      • DIG antibody
      • EINECS 216-806-2 antibody
      • HSDB 7108 antibody
      • Lanadigenin antibody
      • Lanadigigenin antibody
      • ST056392 antibody
      see all

    图片

    • Immunohistochemistry (Frozen sections) - HRP Anti-Digoxigenin antibody [HRP.21H8] (ab6212)
      Immunohistochemistry (Frozen sections) - HRP Anti-Digoxigenin antibody [HRP.21H8] (ab6212)

      ab6212 at a dilution of 1/1000 staining Digoxigenin from human pancreatic tissue sections by immunohistochemistry (frozen). The antibody was incubated with the tissue for 2 hours.

      This image is courtesy of an Abreview submitted by an anonymous researcher on 9 December 2005.

      See Abreview

    文献 (18)

    发表研究结果有使用 ab6212？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab6212 被引用在 18 文献中.

    • Li MK  et al. Long non-coding RNA MEG3 suppresses epithelial-to-mesenchymal transition by inhibiting the PSAT1-dependent GSK-3ß/Snail signaling pathway in esophageal squamous cell carcinoma. Oncol Rep 44:2130-2142 (2020). PubMed: 32901893
    • Shao G  et al. lncRNA CASC9 positively regulates CHK1 to promote breast cancer cell proliferation and survival through sponging the miR-195/497 cluster. Int J Oncol 54:1665-1675 (2019). PubMed: 30816435
    • Filer JE  et al. A Nuclease Protection ELISA Assay for Colorimetric and Electrochemical Detection of Nucleic Acids. Anal Methods 11:1027-1034 (2019). PubMed: 31656535
    • Chen W  et al. Effects of Yangzheng Sanjie Decoction-containing serum mediated by microRNA-7 on cell proliferation and apoptosis in gastric cancer. Oncol Lett 15:3621-3629 (2018). PubMed: 29467883
    • Manjon C  et al. Unravelling the Molecular Determinants of Bee Sensitivity to Neonicotinoid Insecticides. Curr Biol 28:1137-1143.e5 (2018). PubMed: 29576476
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 17 Abreviews or Q&A

    Other (Northern Blot) abreview for Anti-Digoxigenin antibody [HRP.21H8] (HRP)

     Good
    Abreviews
    abreview image
    Application
    Other
    Sample
    Influenza A Cell lysate - other (in vitro RNA transcription for nothern blot)
    Read More

    Abcam user community

    Verified customer

    提交于 Feb 15 2019

    Question

    I would like a certificate of compliance for the following product: Catalog # ab6212 Anti-Digoxigenin antibody [HRP.21H8] (HRP) Lot # GR63837-5 Thank you!

    Read More
    Answer

    Thank you for contacting us.

    I have attached the CoC for ab6212 to this email.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    Thank you for your reply.

    I have received the data from this customer.

    Attached please find the protocol (sheet1), results(sheet2) and modified ELISA schematic (tif)fromthis customer.

    Would you please help this customer check the results?

    Read More
    Answer

    Thanks so much for sending the protocol, data and schematic image. That is all very helpful to see.

    From previous email, it was noted that lot GR40978-1 produced a linear curve while lots GR44124-3, GF63837-3 and GR63837-4 produced a plateau instead. Comparing the data provided using these different lots I am not able to see a significant difference between the raw OD readings from these different lots. All curves seem consistant, showing a minimum detection limit of about 1000ng/ml

    Read More

    Question

    Thank you for your reply.
    Our customer recently has ordered ab6212 with lot# GR62763-3 and GR62763-4 (requesting 2 different lot when putting the order).
    Shehad usedbothlot# gr40978-1 (two vials, good results) andlot#Gr44124-3 (two vial, unidealresults and data different from lot# gr40978-1) in ELISA application. In order to confirm her experimental data, she would like to obtain two different lot of ab612 to reconduct. Therefore, she would like to confirm thatwhether these two lotsare the same batch of manufacture (she wants 2 independent batch)? How many stock with lot# GR62763-3 and GR62763-4? If the experiment set up ready, she calims that 10 vials may be needed.

    Read More
    Answer

    Thank you for your enquiry.

    Regarding lots GR62763-3 and GR62763-4 for ab6212: These are treated as 2 different lots in our stock system. However we receive this antibody from an outside lab and, from this lab, GR62763-3 and GR62763-4 are not produced in separate production runs. They are part of the same production run.


    I hope this helps. Please contact me again if you have any further questions.

    Read More

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Digoxigenin antibody [HRP.21H8] (HRP)

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Pancreas)
    Specification
    Pancreas
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    None
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jun 01 2011

    Immunohistochemistry (Frozen sections) abreview for Anti-Digoxigenin antibody [HRP.21H8] (HRP)

     Good
    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (brain)
    Specification
    brain
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 20% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Mar 31 2009

    Question

    ab6212 (Mouse monoclonal [HRP.21H8] to Digoxigenin (HRP)) What is the concentration of this antibody?

    Read More
    Answer

    Thank you for your patience in this matter. I have received a reply from the originator of ab6212. The concentration of this product is lot dependent, usually between 0.2 - 0.5 mg/ml. The concentration of our present lot is ~0.4 mg/ml. Please don't hesitate to contact us again if you have further questions.

    Read More

    Question

    What concentration of antibody was used in In Situ Hybridization?

    Read More
    Answer

    Thank you for your enquiry. I have received word from the originator that they have not specifically tested this product in in situ hybridization. Please see their response below: This product was used successfully in IHC at the dilution of 1/1000. For in situ hybridization, we don't have any results, however we assume that many customers used it for this application. We recommend the dilution of 1/500 to 1/1000. I have removed ISH from the datasheet as a tested application; however, there doesn't seem to be any reason it won't work. Please let me know if you have any problems.

    Read More

    Question

    ab6212 = Digoxigenin antibody [HRP.21H8] Ab concentration? Immunogen and specificity of Ab? Need to contact originator of ab6212.

    Read More
    Answer

    Thank you for your patience. Regarding ab6212 (Digoxigenin antibody [HRP.21H8]), here is the requested information: antibody concentration: 0.3 mg/ml immunogen: digoxigenin coupled to KLH (details unavailable) I hope this information helps. Please don't hesitate to contact me again if you have further questions.

    Read More

    Immunohistochemistry (Frozen sections) abreview for Anti-Digoxigenin antibody [HRP.21H8] (HRP)

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (in situ hybridization in pancreas)
    Specification
    in situ hybridization in pancreas
    Fixative
    Paraformaldehyde
    Blocking step
    Other as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1
    Read More

    Abcam user community

    Verified customer

    提交于 Dec 09 2005

    1-10 of 17 Abreviews or Q&A

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