HRP Anti-Digoxigenin抗体[HRP.21H8] (ab6212)
![Immunohistochemistry (Frozen sections) - HRP Anti-Digoxigenin antibody [HRP.21H8] (ab6212)](https://www.abcam.cn/ps/products/6/ab6212/Images/ab6212-12175-anti-digoxigenin-antibody-hrp21h8-hrp-immunohistochemistry.jpg "Immunohistochemistry (Frozen sections) - HRP Anti-Digoxigenin antibody [HRP.21H8] (ab6212)")
Key features and details
- HRP Mouse monoclonal [HRP.21H8] to Digoxigenin
- Suitable for: WB, ELISA, IHC-P, IHC-Fr
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG1
概述
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产品名称
HRP Anti-Digoxigenin抗体[HRP.21H8]
参阅全部 Digoxigenin 一抗 -
描述
HRP小鼠单克隆抗体[HRP.21H8] to Digoxigenin -
宿主
Mouse -
偶联物
HRP -
经测试应用
适用于: WB, ELISA, IHC-P, IHC-Frmore details -
种属反应性
与反应: Species independent -
免疫原
Chemical/ Small Molecule conjugated to bovine serum albumin.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.40
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 50% Glycerol, 1% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
HRP.21H8 -
骨髓瘤
unknown -
同种型
IgG1 -
轻链类型
kappa -
研究领域
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应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/5000.
|
|
| ELISA |
1/1000 - 1/10000.
|
|
| IHC-P | (1) |
1/200 - 1/2000. Use in combination with tyramide signal amplification.
|
| IHC-Fr | (2) |
1/200 - 1/2000. 1/200 - 1/2000. Use in combination with tyramide signal amplification.
|
| 说明 |
|---|
|
WB
1/500 - 1/5000. |
|
ELISA
1/1000 - 1/10000. |
|
IHC-P
1/200 - 1/2000. Use in combination with tyramide signal amplification. |
|
IHC-Fr
1/200 - 1/2000. 1/200 - 1/2000. Use in combination with tyramide signal amplification. |
靶标
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相关性
Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea and Digitalis lanata. Digoxigenin is chemically closely related to Digoxin, the cardiac glycoside used for the treatment of various heart diseases. The term 'genin' at the end of Digoxigenin, refers to only the aglycone portion (without the sugar) part of the molecule,thus Digoxigenin is the steroid component of Digoxin, - minus the (digitose) sugar residues. DIG can be covalently added to proteins or nucleic acids which makes it very useful in diverse applications. -
别名
- 1672-46-4 antibody
- BRN 0096479 antibody
- DIG antibody
see all
图片
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Immunohistochemistry (Frozen sections) - HRP Anti-Digoxigenin antibody [HRP.21H8] (ab6212)
ab6212 at a dilution of 1/1000 staining Digoxigenin from human pancreatic tissue sections by immunohistochemistry (frozen). The antibody was incubated with the tissue for 2 hours.
This image is courtesy of an Abreview submitted by an anonymous researcher on 9 December 2005.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (18)
ab6212 被引用在 18 文献中.
- Li MK et al. Long non-coding RNA MEG3 suppresses epithelial-to-mesenchymal transition by inhibiting the PSAT1-dependent GSK-3ß/Snail signaling pathway in esophageal squamous cell carcinoma. Oncol Rep 44:2130-2142 (2020). PubMed: 32901893
- Shao G et al. lncRNA CASC9 positively regulates CHK1 to promote breast cancer cell proliferation and survival through sponging the miR-195/497 cluster. Int J Oncol 54:1665-1675 (2019). PubMed: 30816435
- Filer JE et al. A Nuclease Protection ELISA Assay for Colorimetric and Electrochemical Detection of Nucleic Acids. Anal Methods 11:1027-1034 (2019). PubMed: 31656535
- Chen W et al. Effects of Yangzheng Sanjie Decoction-containing serum mediated by microRNA-7 on cell proliferation and apoptosis in gastric cancer. Oncol Lett 15:3621-3629 (2018). PubMed: 29467883
- Manjon C et al. Unravelling the Molecular Determinants of Bee Sensitivity to Neonicotinoid Insecticides. Curr Biol 28:1137-1143.e5 (2018). PubMed: 29576476