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Tags & Cell Markers Epitope Tags Conjugates

HRP Anti-Digoxigenin抗体[HRP.21H8] (ab6212)

  • Datasheet
  • SDS
Reviews (4)Q&A (13)References (18)

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Immunohistochemistry (Frozen sections) - HRP Anti-Digoxigenin antibody [HRP.21H8] (ab6212)

    Key features and details

    • HRP Mouse monoclonal [HRP.21H8] to Digoxigenin
    • Suitable for: WB, ELISA, IHC-P, IHC-Fr
    • Reacts with: Species independent
    • Conjugation: HRP
    • Isotype: IgG1

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    概述

    • 产品名称

      HRP Anti-Digoxigenin抗体[HRP.21H8]
      参阅全部 Digoxigenin 一抗
    • 描述

      HRP小鼠单克隆抗体[HRP.21H8] to Digoxigenin
    • 宿主

      Mouse
    • 偶联物

      HRP
    • 经测试应用

      适用于: WB, ELISA, IHC-P, IHC-Frmore details
    • 种属反应性

      与反应: Species independent
    • 免疫原

      Chemical/ Small Molecule conjugated to bovine serum albumin.

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C.
    • 存储溶液

      pH: 7.40
      Preservative: 0.01% Thimerosal (merthiolate)
      Constituents: PBS, 50% Glycerol, 1% BSA
    • Concentration information loading...
    • 纯度

      Protein A purified
    • 克隆

      单克隆
    • 克隆编号

      HRP.21H8
    • 骨髓瘤

      unknown
    • 同种型

      IgG1
    • 轻链类型

      kappa
    • 研究领域

      • Tags & Cell Markers
      • Epitope Tags
      • Conjugates

    相关产品

    • Related Products

      • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
      • TMB ELISA Substrate (High Sensitivity) (ab171523)
      • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
      • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
      • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
      • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
      • 450 nm Stop Solution for TMB Substrate (ab171529)
      • 650 nm Stop Solution for TMB Substrate (ab171531)
      • Immunoassay Blocking Buffer (ab171534)
      • Immunoassay Blocking (BSA Free) (ab171535)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab6212于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    1/500 - 1/5000.
    ELISA
    1/1000 - 1/10000.
    IHC-P (1)
    1/200 - 1/2000. Use in combination with tyramide signal amplification.
    IHC-Fr (2)
    1/200 - 1/2000. 1/200 - 1/2000. Use in combination with tyramide signal amplification.
    说明
    WB
    1/500 - 1/5000.
    ELISA
    1/1000 - 1/10000.
    IHC-P
    1/200 - 1/2000. Use in combination with tyramide signal amplification.
    IHC-Fr
    1/200 - 1/2000. 1/200 - 1/2000. Use in combination with tyramide signal amplification.

    靶标

    • 相关性

      Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea and Digitalis lanata. Digoxigenin is chemically closely related to Digoxin, the cardiac glycoside used for the treatment of various heart diseases. The term 'genin' at the end of Digoxigenin, refers to only the aglycone portion (without the sugar) part of the molecule,thus Digoxigenin is the steroid component of Digoxin, - minus the (digitose) sugar residues. DIG can be covalently added to proteins or nucleic acids which makes it very useful in diverse applications.
    • 别名

      • 1672-46-4 antibody
      • BRN 0096479 antibody
      • DIG antibody
      • EINECS 216-806-2 antibody
      • HSDB 7108 antibody
      • Lanadigenin antibody
      • Lanadigigenin antibody
      • ST056392 antibody
      see all

    图片

    • Immunohistochemistry (Frozen sections) - HRP Anti-Digoxigenin antibody [HRP.21H8] (ab6212)
      Immunohistochemistry (Frozen sections) - HRP Anti-Digoxigenin antibody [HRP.21H8] (ab6212)

      ab6212 at a dilution of 1/1000 staining Digoxigenin from human pancreatic tissue sections by immunohistochemistry (frozen). The antibody was incubated with the tissue for 2 hours.

      This image is courtesy of an Abreview submitted by an anonymous researcher on 9 December 2005.

      See Abreview

    实验方案

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (18)

    发表研究结果有使用 ab6212?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab6212 被引用在 18 文献中.

    • Li MK  et al. Long non-coding RNA MEG3 suppresses epithelial-to-mesenchymal transition by inhibiting the PSAT1-dependent GSK-3ß/Snail signaling pathway in esophageal squamous cell carcinoma. Oncol Rep 44:2130-2142 (2020). PubMed: 32901893
    • Shao G  et al. lncRNA CASC9 positively regulates CHK1 to promote breast cancer cell proliferation and survival through sponging the miR-195/497 cluster. Int J Oncol 54:1665-1675 (2019). PubMed: 30816435
    • Filer JE  et al. A Nuclease Protection ELISA Assay for Colorimetric and Electrochemical Detection of Nucleic Acids. Anal Methods 11:1027-1034 (2019). PubMed: 31656535
    • Chen W  et al. Effects of Yangzheng Sanjie Decoction-containing serum mediated by microRNA-7 on cell proliferation and apoptosis in gastric cancer. Oncol Lett 15:3621-3629 (2018). PubMed: 29467883
    • Manjon C  et al. Unravelling the Molecular Determinants of Bee Sensitivity to Neonicotinoid Insecticides. Curr Biol 28:1137-1143.e5 (2018). PubMed: 29576476
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-Digoxigenin antibody [HRP.21H8] (HRP)

    Good
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (brain)
    Specification
    brain
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 20% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Mar 31 2009

    Immunohistochemistry (Frozen sections) abreview for Anti-Digoxigenin antibody [HRP.21H8] (HRP)

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (in situ hybridization in pancreas)
    Specification
    in situ hybridization in pancreas
    Fixative
    Paraformaldehyde
    Blocking step
    Other as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1
    Read More

    Abcam user community

    Verified customer

    提交于 Dec 09 2005

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