HRP Anti-DDDDK tag (Binds to FLAG® tag sequence)抗体(ab1238)
Key features and details
- HRP Goat polyclonal to DDDDK tag (Binds to FLAG® tag sequence)
- Suitable for: ELISA, WB, ICC
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG
概述
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产品名称
HRP Anti-DDDDK tag (Binds to FLAG® tag sequence)抗体
参阅全部 DDDDK tag (Binds to FLAG® tag sequence) 一抗 -
描述
HRP山羊多克隆抗体to DDDDK tag (Binds to FLAG® tag sequence) -
宿主
Goat -
偶联物
HRP -
经测试应用
适用于: ELISA, WB, ICCmore details -
种属反应性
与反应: Species independent -
免疫原
Full length native protein (purified) corresponding to DDDDK tag conjugated to Keyhole Limpet Haemocyanin (KLH).
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常规说明
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.00
Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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应用
Our Abpromise guarantee covers the use of ab1238 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
ELISA | 1/10000 - 1/100000. | |
WB | 1/1000 - 1/30000. | |
ICC | 1/200 - 1/500. |
靶标
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相关性
This is a useful tool for the localisation and characterisation of DDDDK tagged proteins (Binds to FLAG® tag sequence). -
别名
- DDDDK epitope tag antibody
- DDDK antibody
- ddk antibody
see all
图片
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Immunocytochemistry - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1238)Image from Harvey SA et al, Development. 2010 Apr;137(7):1127-35, Fig 1A. DOI 10.1242/dev.046318
Anti-FLAG® immunostaining (using ab1238) of ntl:FLAG® BAC transgenic zebrafish embryos shows expression in the margin at 6 hours post fertilisation.
实验方案
数据表及文件
文献 (14)
ab1238 被引用在 14 文献中.
- Feng S et al. Tripartite motif-containing 14 (TRIM14) promotes epithelial-mesenchymal transition via ZEB2 in glioblastoma cells. J Exp Clin Cancer Res 38:57 (2019). PubMed: 30728039
- Graindorge A et al. In-cell identification and measurement of RNA-protein interactions. Nat Commun 10:5317 (2019). PubMed: 31757954
- Volpe M et al. Regulation of the anaphase promoting complex/cyclosome by the degradation of its unassembled catalytic subunit, Apc11. FASEB J 33:9752-9761 (2019). PubMed: 31162950
- Emmer BT et al. The cargo receptor SURF4 promotes the efficient cellular secretion of PCSK9. Elife 7:N/A (2018). PubMed: 30251625
- Mao SQ et al. DNA G-quadruplex structures mold the DNA methylome. Nat Struct Mol Biol 25:951-957 (2018). PubMed: 30275516