HRP Anti-6X His tag®抗体(ab3553)
Key features and details
- HRP Chicken polyclonal to 6X His tag®
- Suitable for: WB, ELISA
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgY
概述
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产品名称
HRP Anti-6X His tag®抗体
参阅全部 6X His tag® 一抗 -
描述
HRP鸡多克隆抗体to 6X His tag® -
宿主
Chicken -
偶联物
HRP -
经测试应用
适用于: WB, ELISAmore details -
种属反应性
与反应: Species independent -
免疫原
Synthetic peptide corresponding to 6X His tag® conjugated to keyhole limpet haemocyanin.
-
常规说明
HIS-TAG® is a trademark of EMD Biosciences, Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
骨髓瘤
unknown -
同种型
IgY -
轻链类型
unknown -
研究领域
相关产品
-
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应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use at an assay dependent concentration. 1/1000.
|
| ELISA |
Use at an assay dependent concentration. a 1/10000 dilution gave an O.D.= 1.0 in a 15 minute reaction against peptide conjugated to a different carrier than used for anti-peptide purification.
|
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. 1/1000. |
|
ELISA
Use at an assay dependent concentration. a 1/10000 dilution gave an O.D.= 1.0 in a 15 minute reaction against peptide conjugated to a different carrier than used for anti-peptide purification. |
靶标
-
相关性
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. -
细胞定位
Depends upon the localization of the parent protein tagged with hexahistidine. -
别名
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
数据表及文件
-
SDS download
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Datasheet download
文献 (6)
ab3553 被引用在 6 文献中.
- Manning JA et al. Dietary sodium modulates nephropathy in Nedd4-2-deficient mice. Cell Death Differ 27:1832-1843 (2020). PubMed: 31802037
- Burnside D et al. In Silico Engineering of Synthetic Binding Proteins from Random Amino Acid Sequences. iScience 11:375-387 (2019). PubMed: 30660105
- Miao J et al. Wnt/ß-catenin/RAS signaling mediates age-related renal fibrosis and is associated with mitochondrial dysfunction. Aging Cell 18:e13004 (2019). PubMed: 31318148
- Dragovic SM et al. Immunization with AgTRIO, a Protein in Anopheles Saliva, Contributes to Protection against Plasmodium Infection in Mice. Cell Host Microbe 23:523-535.e5 (2018). PubMed: 29649443
- Henshall TL et al. Deletion of Nedd4-2 results in progressive kidney disease in mice. Cell Death Differ 24:2150-2160 (2017). IHC-P ; Mouse . PubMed: 28862701
- Allan CM et al. A conserved START domain coenzyme Q-binding polypeptide is required for efficient Q biosynthesis, respiratory electron transport, and antioxidant function in Saccharomyces cerevisiae. Biochim Biophys Acta 1831:776-791 (2013). PubMed: 23270816