Application
Western blot
Sample
Human Recombinant protein (His-tagged recombinant protein produced in HEK293T)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
25 µg
Specification
His-tagged recombinant protein produced in HEK293T
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Secondary antibody
None used
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 36 kDa Non-specific: none kDa
Positive control
His-tagged protein is present in conditioned media from HEK293T cells
Additional data
Additional Notes
Image shows western blotting of conditioned media from HEK293T cells producing His-tagged recombinant protein.
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提交于 Jan 24 2022