HRP Anti-3-Nitrotyrosine抗体[7A12AF6] (ab198491)
Key features and details
- HRP Mouse monoclonal [7A12AF6] to 3-Nitrotyrosine
- Suitable for: WB
- Reacts with: Cow, Human
- Conjugation: HRP
- Isotype: IgG2b
Related conjugates and formulations
概述
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产品名称
HRP Anti-3-Nitrotyrosine抗体[7A12AF6]
参阅全部 3-Nitrotyrosine 一抗 -
描述
HRP小鼠单克隆抗体[7A12AF6] to 3-Nitrotyrosine -
宿主
Mouse -
偶联物
HRP -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Cow, Human
预测可用于: Mouse, Rat -
免疫原
Chemical/ Small Molecule. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Nitrated liver homogenate and nitrated bovine serum albumin.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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纯度
Proprietary Purification -
纯化说明
The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Purity >95% by SDS-PAGE. -
克隆
单克隆 -
克隆编号
7A12AF6 -
同种型
IgG2b -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab198491于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/5000.
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说明 |
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WB
1/5000. |
靶标
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相关性
Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling. While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase, myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Various studies have shown that protein tyrosinenitration is limited to specific proteins and that the process is selective. For example, exposure of human surfactant protein A, SP-A, to oxygen-nitrogen intermediates generated by activated alveolar macrophages resulted in specific nitration of SP-A at tyrosines 164 and 166, while addition of 1.2 mMCO 2 resulted in additional nitration at tyrosine 161. The presence of nitrotyrosine-containing proteins has shown high correlation to disease states such as atherosclerosis, Alzheimer’s disease, Parkinson’s disease and amyotrophic lateral sclerosis. -
别名
- 3-Nitrotyrosine antibody
- 3-NT antibody
- Nitrotyrosine antibody
图片
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All lanes : HRP Anti-3-Nitrotyrosine antibody [7A12AF6] (ab198491) at 1/5000 dilution
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Nitrated Human Liver Homogenate Standard (ab131380)
Lane 3 : Bovine Serum Albumin
Lane 4 :Bovine Serum Albumin (nitrated ) (ab131379)
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 1 secondThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab198491 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab198491 被引用在 1 文献中.
- Zhang XS et al. Fucoxanthin Mitigates Subarachnoid Hemorrhage-Induced Oxidative Damage via Sirtuin 1-Dependent Pathway. Mol Neurobiol 57:5286-5298 (2020). PubMed: 32876840