概述

  • 产品名称
    Anti-HPV16 L1抗体[CamVir 1]
    参阅全部 HPV16 L1 一抗
  • 描述
    小鼠单克隆抗体[CamVir 1] to HPV16 L1
  • 宿主
    Mouse
  • 特异性
    The antibody reacts with a 56 kDa protein in cells infected with L1-vaccinia virus, the protein being present in a predominantly nuclear location. The antibody reacts very strongly with biopsy specimins containing HPV-16 or -33; very weak reactions were occasionally observed with biopsy specimens or smears containing HPV-6 or HPV-11. Cross-reacts with HPV37.
  • 经测试应用
    适用于: ELISA, WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human papillomavirus
  • 免疫原

    Human papilloma virus type 16, major capsid protein L1.

性能

应用

Our Abpromise guarantee covers the use of ab69 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration. PubMed: 20600940
WB Use at an assay dependent concentration. Predicted molecular weight: 56 kDa.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

靶标

  • 相关性
    L1 is a major capsid protein of type 16 human papilloma virus. Infection with specific types of HPV has been associated with an increased risk of developing cervical neoplasia. HPV types 6 and 11 have been associated with relatively benign diseases such as genital warts but types 16 and 18 are strongly associated with cervical, vaginal, and vulvar malignancies.
  • 细胞定位
    Virion. Host Nucleus.
  • 数据库链接
    • 别名
      • HPV-16 antibody
      • HPV-16 capsid antibody
      • HPV16 capsid protein antibody
      • HPV16 L1 antibody
      • HPV16 major capsid protein L1 antibody
      • Human papillomavirus type 16 L1 antibody
      • Human papillomavirus type 16 major capsid protein L1 antibody
      • L1 antibody
      • Major capsid L1 protein antibody
      • Major capsid protein antibody
      • Major capsid protein L1 antibody
      see all

    文献

    This product has been referenced in:
    • Day PM  et al. Interferon Gamma Prevents Infectious Entry of Human Papillomavirus 16 via an L2-Dependent Mechanism. J Virol 91:N/A (2017). WB . Read more (PubMed: 28250129) »
    • Grant BD  et al. A paper-based immunoassay to determine HPV vaccination status at the point-of-care. Vaccine 34:5656-5663 (2016). Read more (PubMed: 27667331) »
    See all 14 Publications for this product

    客户评价及客户问答

    1-10 of 13 Abreviews or Q&A

    Answer

    Thank you for contacting us.

    Unfortunately, we do not have any other information about the binding site, I am afraid!. The CamVir1 clone is a published clone, please check the literature if anybody else have published any more details about the epitope.

    I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question
    Answer

    Thank you for contacting us.

    The exact epitope sequence has been mapped. The immunogen is a gel-purified HPV-18 E6-b galactosidase fusion protein.

    Please check the link below of original publication;

    http://vir.sgmjournals.org/content/68/5/1351.full.pdf

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Question
    Answer

    Thank you for contacting us.

    Please find attached the requested paper.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Answer

    Thank you for contacting us.

    The anti-HPV16 L1 antibody [CamVir 1] (ab69) has shown some cross reactivity with other HPV types other than 16.The antibody reacts very strongly with biopsy specimens containing HPV-16 or -33, whilstvery weak reactionshave occasionally observed with biopsy specimens or smears containing HPV-6 or HPV-11. There has also been cross reactivity observed with HPV37.

    I hope this information has been of help. If you have any other questions, please do not hesitate to ask.

    Read More

    Answer

    Gracias por contactarnos de nuevo.

    ¿Podrías por favor indicarme el número de pedido en el que se enviaron los anticuerpos? ¿En qué estado se recibieron? ¿Nada más recibirlos se alicuotaron y guardaron a -20C?

    ¿En qué aplicación y especies se están usando?

    Te agradezco que nos facilites estos detalles para poder entender mejor qué ha podido ocurrir para que ninguno de los tres anticuerpos funcione.

    Gracias por la colaboración. Quedo a la espera de tu respuesta.

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    Question
    Answer

    Unfortunately shipment of this order is being held up by our accounts team.

    Our accounting department can be contacted by email at mailto:us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to theorder reference numberin any correspondence with our accounting department.

    I hope this is helpful. Please contact me again with any technical questions you may have.

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    Answer

    Thank you for contacting us.


    If I understand you correctly. The anti-mouse IgG would recognize your purified normal mouse IgG or it would recognize Ab69. These, in turn, would be recognized by goat anti-mouse HRP. However if you are to use ab69 against HPV in mouse tissues your detection antibody (goat anti-mouse IgG-HRP) should IgG2a specific,as it may recognize both ab69 and the Fc receptors in the tissue thereby creating a false positive.

    It seems that youare proposing a sandwich ELISA. These can be difficult to optimize. The antibodies must be detecting different epitopes of the antigen so they do not inferfere with the other antibody binding. We are unable to guarantee our antibodies in sandwich ELISA unless they have been specifically tested in that application.

    If ab69 is your detection


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Answer

    Thank you for contacting Abcam. I have attached the MSDS for all except ab500, ChIP kit. I am still waiting for the laboratory to get back to me on that one and I will send it on as soon as I get it. If there is anything else I can do, please let me know.

    Read More

    Answer

    Thank you for your inquiry. Both Anti-HPV16 L1 recognise HPV Type 16, L1 (major capsid protein). Unfortunately, we do not currently have more information about the epitopes for those monoclonal antibodies. We will update the on-line datasheet for this product as soon as we receive additional data. We apologize for any inconvenience this may cause.

    Read More

    Answer

    Thank you for your email. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to test an antibody in an application for which we do not have any information, please let us know how you get on and in return we will award you 100 points with the Abcam Loyalty Scheme which can be redeemed on a number of rewards. If you have any additional questions, please contact us again.

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    1-10 of 13 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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