Anti-HPV16 E6 + HPV18 E6抗体[C1P5] (ab70)
Key features and details
- Mouse monoclonal [C1P5] to HPV16 E6 + HPV18 E6
- Suitable for: WB, IP, IHC-P
- Reacts with: Human papillomavirus
- Isotype: IgG1
概述
-
产品名称
Anti-HPV16 E6 + HPV18 E6抗体[C1P5]
参阅全部 HPV16 E6 + HPV18 E6 一抗 -
描述
小鼠单克隆抗体[C1P5] to HPV16 E6 + HPV18 E6 -
宿主
Mouse -
经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Human papillomavirus -
免疫原
Gel-purified HPV-18 E6-beta galactosidase fusion protein.
-
阳性对照
- Human cervical cancer cells.
-
常规说明
Clinical relevance: Detection of HPV in cervical smears and biopsies. Uses: Analysis of E6 expression in cell transformation studies.
The E6 proteins are known to form oligomers; PMID19917295, PMID17209544.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
C1P5 -
骨髓瘤
NS1 -
同种型
IgG1 -
轻链类型
unknown -
研究领域
相关产品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
WB |
Use at an assay dependent concentration. Detects a band of approximately 16.5 kDa.
16.5 kDa band is HPV-18, 17 kDa band is HPV-16. The E6 proteins are known to form oligomers; PMID19917295, PMID17209544. |
|
IP |
Use at an assay dependent concentration.
|
|
IHC-P | (1) |
Use at an assay dependent concentration.
|
说明 |
---|
WB
Use at an assay dependent concentration. Detects a band of approximately 16.5 kDa. 16.5 kDa band is HPV-18, 17 kDa band is HPV-16. The E6 proteins are known to form oligomers; PMID19917295, PMID17209544. |
IP
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
靶标
-
相关性
Human Papilloma virus (HPV) is responsible for the development of type and tissue specific papilloma in the oral cavity and in the larynx. In the skin papilloma virus can cause different types of warts and in the ano-genital region it is associated with condyloma or carcinoma. HPV types 1,6 and 11 usually are associated with benign transformations of the tissues, while e.g. HPV types 16, 18 and 31 seem to be responsible for the development of some carcinoma like cervical carcinoma. The role of HPV in non-melanoic tumours of the skin is under discussion. Protein E6 is a Transcriptional transactivator, binding double stranded DNA. It has transforming activity inactivating, with E6-AP ubiquitin-protein ligase, the human TP53/p53 tumor suppressor protein by targeting it for degradation. -
数据库链接
- Entrez Gene: 1489078 Human papillomavirus
- SwissProt: P03126 Human papillomavirus
- SwissProt: P06463 Human papillomavirus
-
别名
- E6 antibody
- Human Papilloma Virus antibody
- Human papillomavirus type 16 E6 antibody
see all
图片
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPV16 E6 + HPV18 E6 antibody [C1P5] (ab70)Image courtesy of Mr Allan Wang
Formalin-fixed, paraffin-embedded human uterine cervix squamous cell carcinoma tissue stained for HPV16 E6 + HPV18 E6 using ab70 at 1/200 dilution in immunohistochemical analysis.
Incubated with primary antibody for 1 hour at 25°C.
Heat mediated antigen retrieval at pH 6.
-
Western blot - Anti-HPV16 E6 + HPV18 E6 antibody [C1P5] (ab70)Image from Zhang Y et al, J Biol Chem. 2005 Sep 30;280(39):33165-77. Epub 2005 Jun 27, Fig 9. DOI 10.1074/jbc.M505124200Subconfluent proliferating MCF-7 or T47D cells were transiently transfected overnight with empty pcDNA3 vector, with FLAG wild-type E6 or zinc finger defective mutant E6 (E6-(C66,136G)). The cells were washed, post-incubated for 24 hours to allow gene expression, and harvested for Western blotting to detect the E6 oncoprotein using ab70.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (27)
ab70 被引用在 27 文献中.
- Chen Y et al. In vitro and in vivo growth inhibition of human cervical cancer cells via human papillomavirus E6/E7 mRNAs' cleavage by CRISPR/Cas13a system. Antiviral Res 178:104794 (2020). PubMed: 32298665
- Li C et al. Reprogrammed CRISPR-Cas13a targeting the HPV16/18 E6 gene inhibits proliferation and induces apoptosis in E6-transformed keratinocytes. Exp Ther Med 19:3856-3860 (2020). PubMed: 32346451
- Zhang C et al. DNMT1 Enhances the Radiosensitivity of HPV-Positive Head and Neck Squamous Cell Carcinomas via Downregulating SMG1. Onco Targets Ther 13:4201-4211 (2020). PubMed: 32523356
- Jin Y et al. Secretory leukocyte protease inhibitor suppresses HPV E6-expressing HNSCC progression by mediating NF-?B and Akt pathways. Cancer Cell Int 19:220 (2019). PubMed: 31462893
- Li C et al. Phenyllactic acid promotes cell migration and invasion in cervical cancer via IKK/NF-?B-mediated MMP-9 activation. Cancer Cell Int 19:241 (2019). PubMed: 31572058