Anti-HPV16 E6 + HPV18 E6抗体[C1P5] (ab70)

Reviews (2)Q&A (20)References (16)

概述

  • 产品名称
    Anti-HPV16 E6 + HPV18 E6抗体[C1P5]
    参阅全部 HPV16 E6 + HPV18 E6 一抗
  • 描述
    小鼠单克隆抗体[C1P5] to HPV16 E6 + HPV18 E6
  • 宿主
    Mouse
  • 经测试应用
    适用于: WB, IP, IHC-Pmore details
  • 免疫原

    Gel-purified HPV-18 E6-beta galactosidase fusion protein.

  • 阳性对照
    • Human cervical cancer cells.
  • 常规说明

    Clinical relevance: Detection of HPV in cervical smears and biopsies. Uses: Analysis of E6 expression in cell transformation studies.

    The E6 proteins are known to form oligomers; PMID19917295, PMID17209544.

性能

应用

Our Abpromise guarantee covers the use of ab70 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Detects a band of approximately 16.5 kDa.

16.5 kDa band is HPV-18, 17 kDa band is HPV-16.

The E6 proteins are known to form oligomers; PMID19917295, PMID17209544.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

靶标

  • 相关性
    Human Papilloma virus (HPV) is responsible for the development of type and tissue specific papilloma in the oral cavity and in the larynx. In the skin papilloma virus can cause different types of warts and in the ano-genital region it is associated with condyloma or carcinoma. HPV types 1,6 and 11 usually are associated with benign transformations of the tissues, while e.g. HPV types 16, 18 and 31 seem to be responsible for the development of some carcinoma like cervical carcinoma. The role of HPV in non-melanoic tumours of the skin is under discussion. Protein E6 is a Transcriptional transactivator, binding double stranded DNA. It has transforming activity inactivating, with E6-AP ubiquitin-protein ligase, the human TP53/p53 tumor suppressor protein by targeting it for degradation.
  • 别名
    • E6 antibody
    • Human Papilloma Virus antibody
    • Human papillomavirus type 16 E6 antibody
    • Human papillomavirus type 18 E6 antibody
    • Protein E6 antibody
    see all

图片

  • Western blot - Anti-HPV16 E6 + HPV18 E6 antibody [C1P5] (ab70)
    Western blot - Anti-HPV16 E6 + HPV18 E6 antibody [C1P5] (ab70)Image from Zhang Y et al, J Biol Chem. 2005 Sep 30;280(39):33165-77. Epub 2005 Jun 27, Fig 9. DOI 10.1074/jbc.M505124200
    Subconfluent proliferating MCF-7 or T47D cells were transiently transfected overnight with empty pcDNA3 vector, with FLAG wild-type E6 or zinc finger defective mutant E6 (E6-(C66,136G)). The cells were washed, post-incubated for 24 hours to allow gene expression, and harvested for Western blotting to detect the E6 oncoprotein using ab70.

文献

This product has been referenced in:
  • Wang X  et al. Human papillomavirus type 16 E6 oncoprotein promotes proliferation and invasion of non-small cell lung cancer cells through Toll-like receptor 3 signaling pathway. J Med Virol N/A:N/A (2017). WB ; Human . Read more (PubMed: 28480962) »
  • Xi R  et al. HPV16 E6-E7 induces cancer stem-like cells phenotypes in esophageal squamous cell carcinoma through the activation of PI3K/Akt signaling pathway in vitro and in vivo. Oncotarget 7:57050-57065 (2016). WB . Read more (PubMed: 27489353) »
See all 16 Publications for this product

发表研究结果有使用 ab70？请让我们知道，以便我们可以引用本数据表中的参考文章。

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HPV16 E6 + HPV18 E6 antibody [C1P5]

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Uterine cervix, Squamous cell carcinoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6.0
Permeabilization
No
Specification
Uterine cervix, Squamous cell carcinoma
Fixative
Formaldehyde
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Mr. Allan Wang

Verified customer

提交于 Apr 17 2018

Question

Does this antibody contain an Fc region?

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Answer

Thank you for your inquiry

I heard back from the lab that, unfortunately, they do not have any information relating to the structure of the antibody. But this is a standard antibody so I would expect that is does have an Fc region.

I hope this information helps. Please contact us with any other questions.

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Question

ab70: will test in ELISA
send DS link for ab98693

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Answer

Thank you for your phone call.

The link to the datasheet of the secondary antibody is :

https://www.abcam.com/goat-polyclonal-secondary-antibody-to-mouse-igg1-heavy-chain-hrp-pre-adsorbed-ab98693.html


Here is the discount code for ab70 and trying it in ELISA, as well as additional information about our Abtrial program:
DISCOUNT CODE: xxx
Expiration date: xxx
Value: xxx

I am very pleased to hear you would like to accept our offer and test ab70 in ELISA. This code will give you xxx off your next order before the expiration date. To redeem this offer, please submit an Abreview for ELISA and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/abtrial.

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Question

Hi,

I would like to know the differences between the next two antibodies:

Anti-HPV16 E6 + HPV18 E6 antibody [C1P5] (ab70) and

Anti-HPV18 E6 antibody [289-13965] (ab30716)

In both, the description suggest that the antibodies react with HPV 16 E6 & HPV 18 E6, however, the Specificity in antibody ab70 is not described.

Another aspect confusing to me is the fact that the immunogen is the same in both cases: fusion protein-Gel purified HPV 18 E6- beta galactosidase fusion protein.

Please, is really important to me understand the difference between this two options in order to choose the best for my lab.

Best regards,

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Answer

Thank you for contacting us and your interest in our products.

The antibodies ab70 and ab30716 are in fact the same antibody, produced from different sources.I can understand that this is currently not reflected very well on our website but we will be updating this. Thank you for bringing this to our attention.

The antibodies ab70 and ab30716 are both mouse monoclonals produced from the clone C1P5 (also known as 289-13965). Both are capable of detecting both the HPV 18 E6 and HPV 16 E6, this is stated in the title of the antibody or the "specificity" note on the datasheet.

I am sorry for any confusion caused. If you have any further questions, please do not hesitate to contact us again.

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Immunocytochemistry abreview for Anti-HPV16 E6 + HPV18 E6 antibody [C1P5]

 Poor
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
abreview image
Application
Immunocytochemistry
Sample
Cow Cultured Cells (Epithelial cells of cows with BPV and cells free-B)
Specification
Epithelial cells of cows with BPV and cells free-B
Fixative
Paraformaldehyde
Permeabilization
Yes - Tritonx100 (0,1%),
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Aug 31 2012

Question

Our costumer told me that she had already submitted an ABReview for ab70 (ICC application), but today I was visiting ABCAM website and couldn't find it.

So, I would like to know from you:

1- When an ABReview was submitted does it go straight to the site page or not?

2- Did you get my costumer's ABReview for ab70? (She submitted it on August 22).


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Answer

Thank you for your reply.


I have located the Abreview in our system for ab70. It seems that the customer has begun this review, but must provide the product lot number (found on the vial) in order to officially submit the review. Therefore it is in an editing status.


In general, when a review is submitted to Abcam it does not appear on the Abcam website immediately. The Scientific Support team reviews these to make sure they are complete and that the information provided makes sense. We will then publish them on the antibody datasheet.


I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

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Question

I had talked to our costumer and she has already submitted an ABReview for ab70 with her ICC/ IF results.

She is really happy with the discount for getting a new antibody for free.



She said that will talk to her Lab master about BIOGEN and ABCAM support for the great conclusion of this case and that certainly will be new purchases of ABCAM antibodies in a nearly future.

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Answer

Thank you for the update and I am really happy that the customer was satisfied with the outcome.

If you have any other situations like this or if there is anything else we can help with, then please let me know.

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Question

Was this antibody tested in ELISA to verify specificity? If so, what antigen was used?
What other QC tests were performed?

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Answer

Thank you very much for contacting us a while back about the HPV16E6 + HPV18 E6 antibody ab70.

I sincerely apologize for the extended delay in getting this information for you, and I appreciate your patience while I have been in touch with the production lab regarding this antibody. The antibody is produced in an outside lab, and due to the nature of the antigen there is no regular QC ELISA testing done. Each new lot is tested by SDS-PAGE and an isotype test after production to ensure quality, and noapplication testing isperformed.

I am very sorry for the delay getting back to you, but I hope this information might be useful. If you have any further questions or if there is anything else that we can do for you, please let me know and I'll be happy to help.

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Question


About your questions and suggestions for ab70 antibody, the customer
answers are below:
She did the assay again, changing her protocol as suggest, however the
unspecific staining remains.
answers:
1- Her cells are monolayer, and she fixed with PFA for 20 min
2- The staining is correct, and it is strong enough at negative
control. She did a control using only secundary antibody (FITC)- w/o
primary antibody, and it did not stain. See attached ppt.
3- Yes, she reduced the incubation time of primary antibody (2h),
and even so the negative control remained stained
Please, see attached ppt.
Best regards,

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Answer

Thank you for your reply.

As we have not previously tested this antibody in ICC/IF, we could not guarantee that it would work for this application, as under our Abpromise we only guarantee it to work in WB, IP& IHC-P.

However, I think that the customer may be seeing some specific staining using the antibody, as although there is a signal in the negative control (without HPV or BPV), the signal in the positive cells is much more intense. Although it is difficult to make a accurate comparison, as the images were taken at different magnification.

To help reduce the background, that is being seen in the negative control, I would suggest the following:

1 - Increase blocking to 10%BSA, for 1hr at room temperature.

2 - Reduce the primary antibody concentration to 1/750, for 2 hrs at room temperature.

Hopefully, the above protocol variations will help to improve the staining that is being seen.

If there is anything else I can help you with, please let me know.

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Question

I am currently doing my MSC project on HPV in head and neck cancer and happens to remember you work with abcam. I can see that you have the Anti-HPV16 E6 + HPV18 E6 antibody [C1P5]. I would like to make a comparison of its sensitivity with p16ink4a if you can help me get a sample or from your kindness, a free one please. Let me know what you can do anything for me.
Many thanks

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Answer

Thank you very much for your interest in https://www.abcam.com/ab70 (Anti-HPV16 E6 + HPV18 E6 antibody [C1P5])

To our knowledge,ab70 has not been tested with p16ink4a. Therefore, I can offer a discount off a future purchase if you buy ab70 now, test it with p16ink4a and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab70 with p16ink4a. I will then send a discount code. This code must be issued before purchasing ab70 so please wait for my reply before ordering.

2. Purchase ab70 either by phone, fax, or online (https://www.abcam.com).

3. Test it with p16ink4a.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated!

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: https://www.abcam.com/collaborationdiscount.

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1-10 of 22 Abreviews or Q&A

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