Anti-HPV16 E6 + HPV18 E6抗体[C1P5] (ab70)
概述
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产品名称Anti-HPV16 E6 + HPV18 E6抗体[C1P5]
参阅全部 HPV16 E6 + HPV18 E6 一抗 -
描述小鼠单克隆抗体[C1P5] to HPV16 E6 + HPV18 E6
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宿主Mouse
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经测试应用适用于: WB, IP, IHC-Pmore details
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种属反应性与反应: Human papillomavirus
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免疫原
Gel-purified HPV-18 E6-beta galactosidase fusion protein.
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阳性对照
- Human cervical cancer cells.
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常规说明
Clinical relevance: Detection of HPV in cervical smears and biopsies. Uses: Analysis of E6 expression in cell transformation studies.
The E6 proteins are known to form oligomers; PMID19917295, PMID17209544.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Concentration information loading...
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纯度Protein A purified
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克隆单克隆
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克隆编号C1P5
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骨髓瘤NS1
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同种型IgG1
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轻链类型unknown
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研究领域
相关产品
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Alternative Products
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
Our Abpromise guarantee covers the use of ab70 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
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WB | Use at an assay dependent concentration. Detects a band of approximately 16.5 kDa. 16.5 kDa band is HPV-18, 17 kDa band is HPV-16. The E6 proteins are known to form oligomers; PMID19917295, PMID17209544. |
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IP | Use at an assay dependent concentration. | |
IHC-P | Use at an assay dependent concentration. |
靶标
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相关性Human Papilloma virus (HPV) is responsible for the development of type and tissue specific papilloma in the oral cavity and in the larynx. In the skin papilloma virus can cause different types of warts and in the ano-genital region it is associated with condyloma or carcinoma. HPV types 1,6 and 11 usually are associated with benign transformations of the tissues, while e.g. HPV types 16, 18 and 31 seem to be responsible for the development of some carcinoma like cervical carcinoma. The role of HPV in non-melanoic tumours of the skin is under discussion. Protein E6 is a Transcriptional transactivator, binding double stranded DNA. It has transforming activity inactivating, with E6-AP ubiquitin-protein ligase, the human TP53/p53 tumor suppressor protein by targeting it for degradation.
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数据库链接
- Entrez Gene: 1489078 Human papillomavirus
- SwissProt: P03126 Human papillomavirus
- SwissProt: P06463 Human papillomavirus
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别名
- E6 antibody
- Human Papilloma Virus antibody
- Human papillomavirus type 16 E6 antibody
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图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPV16 E6 + HPV18 E6 antibody [C1P5] (ab70)Image courtesy of Mr Allan Wang
Formalin-fixed, paraffin-embedded human uterine cervix squamous cell carcinoma tissue stained for HPV16 E6 + HPV18 E6 using ab70 at 1/200 dilution in immunohistochemical analysis.
Incubated with primary antibody for 1 hour at 25°C.
Heat mediated antigen retrieval at pH 6.
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Western blot - Anti-HPV16 E6 + HPV18 E6 antibody [C1P5] (ab70)Image from Zhang Y et al, J Biol Chem. 2005 Sep 30;280(39):33165-77. Epub 2005 Jun 27, Fig 9. DOI 10.1074/jbc.M505124200Subconfluent proliferating MCF-7 or T47D cells were transiently transfected overnight with empty pcDNA3 vector, with FLAG wild-type E6 or zinc finger defective mutant E6 (E6-(C66,136G)). The cells were washed, post-incubated for 24 hours to allow gene expression, and harvested for Western blotting to detect the E6 oncoprotein using ab70.
文献
This product has been referenced in:
- Wang X et al. Human papillomavirus type 16 E6 oncoprotein promotes proliferation and invasion of non-small cell lung cancer cells through Toll-like receptor 3 signaling pathway. J Med Virol N/A:N/A (2017). WB ; Human . Read more (PubMed: 28480962) »
- Xi R et al. HPV16 E6-E7 induces cancer stem-like cells phenotypes in esophageal squamous cell carcinoma through the activation of PI3K/Akt signaling pathway in vitro and in vivo. Oncotarget 7:57050-57065 (2016). WB . Read more (PubMed: 27489353) »