Anti-HPV11 E7抗体[9H5] (ab100967)
概述
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产品名称Anti-HPV11 E7抗体[9H5]
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描述小鼠单克隆抗体[9H5] to HPV11 E7
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宿主Mouse
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特异性Reacts in WB, IP, ELISA with HPV11 native E7 protein as well as recombinant E7 protein expressed in bacteria and Cos7 cells.
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经测试应用适用于: WB, IP, ELISA, ICC/IFmore details
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种属反应性与反应: Human papillomavirus
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免疫原
Recombinant full length HPV11 E7 protein purified from bacteria.
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表位Mapped to amino acids 36-70
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阳性对照
- WB: U2OS and HeLa cell lysates ICC/IF: U2OS cells transfected with plasmid expressing HPV-11 E7 protein IP: lysate of U20S cells
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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存储溶液Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4 -
Concentration information loading... -
纯度Protein A purified
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克隆单克隆
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克隆编号9H5
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同种型IgG2a
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研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
Our Abpromise guarantee covers the use of ab100967 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 11 kDa. | |
| IP | 1/500. | |
| ELISA | 1/128000. | |
| ICC/IF | Use at an assay dependent dilution. |
靶标
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相关性Infections by human papillomaviruses (HPV) can cause relatively harmless plantar warts and also more serious genital warts that can increase the risk to women for developing cervical cancer. Infections with HPV11 triggers genital warts. E7 is a protein that is encoded by by HPV11 and is implicated in the disruption of host cells and promoting their proliferation.
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数据库链接
- SwissProt: P04020 Human papillomavirus
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别名
- Human papillomavirus E7 protein antibody
图片
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![Western blot - Anti-HPV11 E7 antibody [9H5] (ab100967)](https://www.abcam.cn/ps/products/100/ab100967/Images/HPV11-E7-Primary-antibodies-ab100967-1.jpg)
Western blot - Anti-HPV11 E7 antibody [9H5] (ab100967)All lanes : Anti-HPV11 E7 antibody [9H5] (ab100967) at 1/1000 dilution
Lanes 1 & 4 : lysate of U20S cells transfected with plasmid expressing HPV11 E7 protein
Lane 2 : lysate of plain U20S cells
Lane 3 : lysate of HeLa cells
Lane 5 : marker
Predicted band size: 11 kDa -
![Immunoprecipitation - Anti-HPV11 E7 antibody [9H5] (ab100967)](https://www.abcam.cn/ps/products/100/ab100967/Images/HPV11-E7-Primary-antibodies-ab100967-2.jpg)
Immunoprecipitation - Anti-HPV11 E7 antibody [9H5] (ab100967)Immunoprecipitation (IP) analysis of HPV11 E7 protein.
Lane 1 represents the marker.
Lane 2 shows detection of E7 protein by ab100967 in lysate of U20S cells.
In lane 3 the lower band is precipitated E7, upper bands are light and heavy chain of the antibody.
Lane 4 is precipitation without ab100967
Lane 5 shows IP experiment with plain U2OS cells. -
![Immunocytochemistry/ Immunofluorescence - Anti-HPV11 E7 antibody [9H5] (ab100967)](https://www.abcam.cn/ps/products/100/ab100967/Images/HPV11-E7-Primary-antibodies-ab100967-3.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HPV11 E7 antibody [9H5] (ab100967)Immunofluorescence (IF) analysis of HPV11 E7 protein in U2OS cells.
IF was carried out using U2OS cells transfected with plasmid expressing HPV11 E7 protein. All the nuclei were stained by DAPI. Green color represents nucleus localized HPV11 E7 detected by ab100967. IF analysis of E7 protein shows similar result also in HeLa cells.
实验方案
文献
ab100967 has not yet been referenced specifically in any publications.
