Anti-HPV11 E7抗体[9H5] (ab100967)
![Western blot - Anti-HPV11 E7 antibody [9H5] (ab100967)](https://www.abcam.cn/ps/products/100/ab100967/Images/ab100967-114077-anti-hpv11-e7-antibody-9h5-western-blot.jpg "Western blot - Anti-HPV11 E7 antibody [9H5] (ab100967)")
Key features and details
- Mouse monoclonal [9H5] to HPV11 E7
- Suitable for: WB, IP, ELISA, ICC/IF
- Reacts with: Human papillomavirus
- Isotype: IgG2a
概述
-
产品名称
Anti-HPV11 E7抗体[9H5] -
描述
小鼠单克隆抗体[9H5] to HPV11 E7 -
宿主
Mouse -
特异性
Reacts in WB, IP, ELISA with HPV11 native E7 protein as well as recombinant E7 protein expressed in bacteria and Cos7 cells. -
经测试应用
适用于: WB, IP, ELISA, ICC/IFmore details -
种属反应性
与反应: Human papillomavirus -
免疫原
Recombinant full length HPV11 E7 protein purified from bacteria.
-
表位
Mapped to amino acids 36-70 -
阳性对照
- WB: U2OS and HeLa cell lysates ICC/IF: U2OS cells transfected with plasmid expressing HPV-11 E7 protein IP: lysate of U20S cells
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
9H5 -
同种型
IgG2a -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab100967于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 11 kDa.
|
|
| IP |
1/500.
|
|
| ELISA |
1/128000.
|
|
| ICC/IF |
Use at an assay dependent dilution.
|
| 说明 |
|---|
|
WB
1/1000. Predicted molecular weight: 11 kDa. |
|
IP
1/500. |
|
ELISA
1/128000. |
|
ICC/IF
Use at an assay dependent dilution. |
靶标
-
相关性
Infections by human papillomaviruses (HPV) can cause relatively harmless plantar warts and also more serious genital warts that can increase the risk to women for developing cervical cancer. Infections with HPV11 triggers genital warts. E7 is a protein that is encoded by by HPV11 and is implicated in the disruption of host cells and promoting their proliferation. -
数据库链接
- SwissProt: P04020 Human papillomavirus
-
别名
- Human papillomavirus E7 protein antibody
图片
-
Western blot - Anti-HPV11 E7 antibody [9H5] (ab100967)All lanes : Anti-HPV11 E7 antibody [9H5] (ab100967) at 1/1000 dilution
Lanes 1 & 4 : lysate of U20S cells transfected with plasmid expressing HPV11 E7 protein
Lane 2 : lysate of plain U20S cells
Lane 3 : lysate of HeLa cells
Lane 5 : marker
Predicted band size: 11 kDa -
![Immunoprecipitation - Anti-HPV11 E7 antibody [9H5] (ab100967)](https://www.abcam.cn/ps/products/100/ab100967/Images/ab100967-114076-anti-hpv11-e7-antibody-9h5-immunoprecipitation.jpg)
Immunoprecipitation - Anti-HPV11 E7 antibody [9H5] (ab100967)Immunoprecipitation (IP) analysis of HPV11 E7 protein.
Lane 1 represents the marker.
Lane 2 shows detection of E7 protein by ab100967 in lysate of U20S cells.
In lane 3 the lower band is precipitated E7, upper bands are light and heavy chain of the antibody.
Lane 4 is precipitation without ab100967
Lane 5 shows IP experiment with plain U2OS cells. -
![Immunocytochemistry/ Immunofluorescence - Anti-HPV11 E7 antibody [9H5] (ab100967)](https://www.abcam.cn/ps/products/100/ab100967/Images/ab100967-114075-anti-hpv11-e7-antibody-9h5-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HPV11 E7 antibody [9H5] (ab100967)Immunofluorescence (IF) analysis of HPV11 E7 protein in U2OS cells.
IF was carried out using U2OS cells transfected with plasmid expressing HPV11 E7 protein. All the nuclei were stained by DAPI. Green color represents nucleus localized HPV11 E7 detected by ab100967. IF analysis of E7 protein shows similar result also in HeLa cells.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab100967 尚未被引用在任何文献中。