Key features and details
- Mouse monoclonal [BPV-1/1H8 + CAMVIR] to HPV
- Suitable for: IHC-P, ELISA
- Reacts with: Human papillomavirus
- Isotype: IgG
产品名称Anti-HPV抗体[BPV-1/1H8 + CAMVIR]
参阅全部 HPV 一抗
描述小鼠单克隆抗体[BPV-1/1H8 + CAMVIR] to HPV
The BPV-1/1H8 clone recognizes HPV-1, 6, 11 and 16. The CAMVIR clone recognizes HPV-16, 18 and 31.
Other HPV isotypes may also be reactive with the Broad Spectrum HPV antibody, but have not been tested.
经测试应用适用于: IHC-P, ELISAmore details
种属反应性与反应: Human papillomavirus
This product was produced with the following immunogens:
Recombinant full length protein. The CAMVIR-1 antibody was raised against the major capsid protein L1 of human papillomavirus type 16, using a recombinant vaccinia virus that expresses the L1 protein.
Tissue, cells or virus. The BPV-1/1H8 antibody was raised against SDS-disrupted bovine papillomavirus type 1(BPV-1)
- IHC-P: Infected cervical biopsy tissue. WB: Control and DCT siRNA transfected HaCaT cell lysate.
Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen. This cocktail is a mix of BPV-1/1H8 (IgG) and CAMVIR (IgG2a).
The broad spectrum HPV antibody was produced (1H8) against SDS-disrupted bovine papillomavirus type 1 (BPV-1) and used to identify the product of the L1 open reading frame (ORF) of BPV-1. IH8 was found to be reactive with purified major capsid protein (MCP). The antibody was tested with ELISA and with an immunofluorescent technique and detected HPV-1, 6, 11, 16, 18, and 31 in formalin-fixed paraffin embedded biopsy specimens. The CAMVIR-1 antibody was raised against the major capsid protein L1 of human papillomavirus type 16, using a recombinant vaccinia virus that expresses the L1 protein, as a target for screening. This antibody reacted with a 56 kilodalton protein in cells infected with L1-vaccinia virus, and the protein was present in HPV16. This antibody cocktail may also work in PAP smears, but has not been tested. A panel of p16 and Ki-67 can be used for further evaluation.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.09% Sodium azide
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克隆编号BPV-1/1H8 + CAMVIR
Our Abpromise guarantee covers the use of ab2417 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/80 - 1/160. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ELISA||Use at an assay dependent concentration.|
相关性Human papillomaviruses (HPV) are small DNA viruses which infect epithelia of the skin and mucosa. Over 90 types have been identified and they mostly cause a variety of benign lesions such as warts and verrucae. However, some subtypes, notably types 16 and 18, 31 and 33, have been confirmed as agents which cause cervical cancer.
- HPV antibody
- Human papillomavirus antibody
ab2417 被引用在 14 文献中.
- Chang CY et al. The Detection and Association of Canine Papillomavirus with Benign and Malignant Skin Lesions in Dogs. Viruses 12:N/A (2020). PubMed: 32028559
- Lange CE et al. Canine papillomavirus types 1 and 2 in classical papillomas: High abundance, different morphological associations and frequent co-infections. Vet J 250:1-5 (2019). PubMed: 31383413
- Gang DG et al. Sebaceous cell differentiation in a canine oral papilloma. J Vet Diagn Invest 30:569-571 (2018). PubMed: 29860939
- Larmour LI et al. A patient derived xenograft model of cervical cancer and cervical dysplasia. PLoS One 13:e0206539 (2018). PubMed: 30365542
- Aksoy P & Meneses PI The Role of DCT in HPV16 Infection of HaCaTs. PLoS One 12:e0170158 (2017). WB . PubMed: 28095444