Anti-HPV抗体[BPV-1/1H8 + CAMVIR] (ab2417)

I recommend ab97023, goat anti-mouse IgG H&L (heavy and light chains), assuming you want an HRP conjugate (horseradish peroxidase) for visualization. We also have fluorochrome conjugates and alkaline phosphatase conjugates, among others.

Thank you for contacting Abcam

Theprotein carrier forHPV Cocktail Broad Spectrumis heat attenuated goat serum.

Please let me know if there is anything else I can help you with.

Thank you for your enquiry and for your patience. It has taken some time to gather the information.

Regardnig ab2417 Anti-HPV antibody [BPV-1/1H8 + CAMVIR], I can confirm thiswas raised against the major capsid protein L1. I have copied below some further information:

The broad spectrum HPV antibody was produced (1H8) against SDS-disrupted bovine papillomavirus type 1 (BPV-1) and used to identify the product of the L1 open reading frame (ORF) of BPV-1. IH8 was found to be reactive with purified major capsid protein (MCP). The antibody was tested with ELISA and with an immunofluorescent technique and detected HPV-1, 6, 11, 16-16, 18, and 31 in formalin-fixed paraffin-embedded biopsy specimens. The CAMVIR-1 antibody was raised against the major capsid protein L1 of human papillomavirus type 16, using a recombinant vaccinia virus that expresses the L1 protein, as a target for screening. This antibody, designated CAMVIR-1, reacted with a 56 kilodalton protein in cells infected with L1-vaccinia virus, and the protein was present in HPV16. This antibody cocktail may also work in PAP smears, but has not been tested. A panel of p16 and Ki-67 can be used for further evaluation.

Regarding ab30908HPV16 L1 antibody [289-16981], this should detectL1 as it was raised against HPV Type 16L1 protein.

The ab75574 Anti-HPV antibody [K1H8],hasbeen sourced and tested externally. I am sorry we have not been able to obtain any further information yet for these antibodies, and therefore I would assume this has regrettably not been specifically tested for detection of L1.

For western blotting, I can confirm that all tested applications covered by our guarantee are listed on the online datasheets which are updated as soon as we receive further information. I can confirm that ab2417 and ab30908 have been tested in WB. The ab75574 has not been tested in WB.

I hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

Thank you for contacting us.

My colleague has created an SDS for ab2417 and it is now available for download from our website. I have also attached it to this email. Please let me know if you need any additional information.

Thanks you for your enquiry. Based on the following publications, there are cases where the HPV expression was found in the cytoplasm and nuclear region. 1) CASE REPORT: Corneal papilloma associated with papillomavirus in a one-humped camel (Camelus dromedarius). Veterinary Ophthalmology. Volume 13, Issue Supplement s1, pages 100–102, September 2010. (Fig 3) http://onlinelibrary.wiley.com/doi/10.1111/j.1463-5224.2010.00795.x/full 2) Clinical, pathological and immunohistochemical findings of bovine　cutaneous papillomatosis. Ankara Üniv Vet Fak Derg, 58, 161-165, 2011 (Fig 2) http://dergiler.ankara.edu.tr/dergiler/11/1565/16994.pdf Therefore, the results you are seeing is not uncommon. Although on our datasheet it does state nuclear, this is information from SwissProt and may not be the case in an actual experiment. I hope this information will be helpful.

Thank you for your enquiry regarding ab2417. After discussing your comments with some experts, we have reviewed the alternative names on the datasheet and amended it. We apologize for any inconvenience caused and thank you for bringing this matter to our attention. If you need any further assistance in the future, please do not hesitate to contact me.  

Thank you for your enquiry. This antibody was used at a 1:80 dilution and the secondary antibody was a MACH3 Mouse AP Polymer. Please bear in mind that different samples and buffers used may require some optimization. I hope this information will be helpful. If there is anything else that I can help you with please do not hesitate to contact me. Have a nice day!

The antibody was used at a concentration of 1:80 in IHC-P. Secondary was AP-polymer. We have not carried out ICC/IF for this antibody. Thus, we are unable to guarantee the suitability of this application. Probably antibody could be further diluted.