Anti-HPV抗体[BPV-1/1H8 + CAMVIR] (ab2417)
Key features and details
- Mouse monoclonal [BPV-1/1H8 + CAMVIR] to HPV
- Suitable for: IHC-P, ELISA
- Reacts with: Human papillomavirus
- Isotype: IgG
概述
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产品名称
Anti-HPV抗体[BPV-1/1H8 + CAMVIR]
参阅全部 HPV 一抗 -
描述
小鼠单克隆抗体[BPV-1/1H8 + CAMVIR] to HPV -
宿主
Mouse -
特异性
The BPV-1/1H8 clone recognizes HPV-1, 6, 11 and 16. The CAMVIR clone recognizes HPV-16, 18 and 31.
Other HPV isotypes may also be reactive with the Broad Spectrum HPV antibody, but have not been tested.
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经测试应用
适用于: IHC-P, ELISAmore details -
种属反应性
与反应: Human papillomavirus -
免疫原
This product was produced with the following immunogens:
Recombinant full length protein. The CAMVIR-1 antibody was raised against the major capsid protein L1 of human papillomavirus type 16, using a recombinant vaccinia virus that expresses the L1 protein.
Tissue, cells or virus. The BPV-1/1H8 antibody was raised against SDS-disrupted bovine papillomavirus type 1(BPV-1) -
阳性对照
- IHC-P: Infected cervical biopsy tissue. WB: Control and DCT siRNA transfected HaCaT cell lysate.
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常规说明
Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen. This cocktail is a mix of BPV-1/1H8 (IgG) and CAMVIR (IgG2a).
The broad spectrum HPV antibody was produced (1H8) against SDS-disrupted bovine papillomavirus type 1 (BPV-1) and used to identify the product of the L1 open reading frame (ORF) of BPV-1. IH8 was found to be reactive with purified major capsid protein (MCP). The antibody was tested with ELISA and with an immunofluorescent technique and detected HPV-1, 6, 11, 16, 18, and 31 in formalin-fixed paraffin embedded biopsy specimens. The CAMVIR-1 antibody was raised against the major capsid protein L1 of human papillomavirus type 16, using a recombinant vaccinia virus that expresses the L1 protein, as a target for screening. This antibody reacted with a 56 kilodalton protein in cells infected with L1-vaccinia virus, and the protein was present in HPV16. This antibody cocktail may also work in PAP smears, but has not been tested. A panel of p16 and Ki-67 can be used for further evaluation.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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克隆
单克隆 -
克隆编号
BPV-1/1H8 + CAMVIR -
骨髓瘤
unknown -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2417 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
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IHC-P |
1/80 - 1/160. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ELISA |
Use at an assay dependent concentration.
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说明 |
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IHC-P
1/80 - 1/160. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ELISA
Use at an assay dependent concentration. |
靶标
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相关性
Human papillomaviruses (HPV) are small DNA viruses which infect epithelia of the skin and mucosa. Over 90 types have been identified and they mostly cause a variety of benign lesions such as warts and verrucae. However, some subtypes, notably types 16 and 18, 31 and 33, have been confirmed as agents which cause cervical cancer. -
细胞定位
Nuclear -
别名
- HPV antibody
- Human papillomavirus antibody
图片
数据表及文件
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SDS download
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Datasheet download
文献 (14)
ab2417 被引用在 14 文献中.
- Chang CY et al. The Detection and Association of Canine Papillomavirus with Benign and Malignant Skin Lesions in Dogs. Viruses 12:N/A (2020). PubMed: 32028559
- Lange CE et al. Canine papillomavirus types 1 and 2 in classical papillomas: High abundance, different morphological associations and frequent co-infections. Vet J 250:1-5 (2019). PubMed: 31383413
- Gang DG et al. Sebaceous cell differentiation in a canine oral papilloma. J Vet Diagn Invest 30:569-571 (2018). PubMed: 29860939
- Larmour LI et al. A patient derived xenograft model of cervical cancer and cervical dysplasia. PLoS One 13:e0206539 (2018). PubMed: 30365542
- Aksoy P & Meneses PI The Role of DCT in HPV16 Infection of HaCaTs. PLoS One 12:e0170158 (2017). WB . PubMed: 28095444