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Microbiology Organism Virus DNA Virus double stranded DNA Virus Human Papillomavirus

Anti-HPV抗体[BPV-1/1H8 + CAMVIR] (ab2417)

  • Datasheet
  • SDS
Reviews (1)Q&A (8)References (14)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPV antibody [BPV-1/1H8 + CAMVIR] (ab2417)

    Key features and details

    • Mouse monoclonal [BPV-1/1H8 + CAMVIR] to HPV
    • Suitable for: IHC-P, ELISA
    • Reacts with: Human papillomavirus
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-HPV抗体[BPV-1/1H8 + CAMVIR]
      参阅全部 HPV 一抗
    • 描述

      小鼠单克隆抗体[BPV-1/1H8 + CAMVIR] to HPV
    • 宿主

      Mouse
    • 特异性

      The BPV-1/1H8 clone recognizes HPV-1, 6, 11 and 16. The CAMVIR clone recognizes HPV-16, 18 and 31.

      Other HPV isotypes may also be reactive with the Broad Spectrum HPV antibody, but have not been tested.

    • 经测试应用

      适用于: IHC-P, ELISAmore details
    • 种属反应性

      与反应: Human papillomavirus
    • 免疫原

      This product was produced with the following immunogens:
      Recombinant full length protein. The CAMVIR-1 antibody was raised against the major capsid protein L1 of human papillomavirus type 16, using a recombinant vaccinia virus that expresses the L1 protein.

      Tissue, cells or virus. The BPV-1/1H8 antibody was raised against SDS-disrupted bovine papillomavirus type 1(BPV-1)

    • 阳性对照

      • IHC-P: Infected cervical biopsy tissue. WB: Control and DCT siRNA transfected HaCaT cell lysate.
    • 常规说明

      Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen. This cocktail is a mix of BPV-1/1H8 (IgG) and CAMVIR (IgG2a).

      The broad spectrum HPV antibody was produced (1H8) against SDS-disrupted bovine papillomavirus type 1 (BPV-1) and used to identify the product of the L1 open reading frame (ORF) of BPV-1. IH8 was found to be reactive with purified major capsid protein (MCP). The antibody was tested with ELISA and with an immunofluorescent technique and detected HPV-1, 6, 11, 16, 18, and 31 in formalin-fixed paraffin embedded biopsy specimens. The CAMVIR-1 antibody was raised against the major capsid protein L1 of human papillomavirus type 16, using a recombinant vaccinia virus that expresses the L1 protein, as a target for screening. This antibody reacted with a 56 kilodalton protein in cells infected with L1-vaccinia virus, and the protein was present in HPV16. This antibody cocktail may also work in PAP smears, but has not been tested. A panel of p16 and Ki-67 can be used for further evaluation.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • 存储溶液

      Preservative: 0.09% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • 克隆

      单克隆
    • 克隆编号

      BPV-1/1H8 + CAMVIR
    • 骨髓瘤

      unknown
    • 同种型

      IgG
    • 研究领域

      • Microbiology
      • Organism
      • Virus
      • DNA Virus
      • double stranded DNA Virus
      • Human Papillomavirus
      • Cancer
      • Oncoproteins/suppressors
      • Viral proteins
      • HPV

    相关产品

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG - Isotype Control (ab37355)

    应用

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab2417 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    IHC-P
    1/80 - 1/160. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    ELISA
    Use at an assay dependent concentration.
    说明
    IHC-P
    1/80 - 1/160. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    ELISA
    Use at an assay dependent concentration.

    靶标

    • 相关性

      Human papillomaviruses (HPV) are small DNA viruses which infect epithelia of the skin and mucosa. Over 90 types have been identified and they mostly cause a variety of benign lesions such as warts and verrucae. However, some subtypes, notably types 16 and 18, 31 and 33, have been confirmed as agents which cause cervical cancer.
    • 细胞定位

      Nuclear
    • 别名

      • HPV antibody
      • Human papillomavirus antibody

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPV antibody [BPV-1/1H8 + CAMVIR] (ab2417)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPV antibody [BPV-1/1H8 + CAMVIR] (ab2417)
      ab2417 staining HPV-infected human cervix tissue sections.

    实验方案

    • Immunohistochemistry protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (14)

    发表研究结果有使用 ab2417?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2417 被引用在 14 文献中.

    • Chang CY  et al. The Detection and Association of Canine Papillomavirus with Benign and Malignant Skin Lesions in Dogs. Viruses 12:N/A (2020). PubMed: 32028559
    • Lange CE  et al. Canine papillomavirus types 1 and 2 in classical papillomas: High abundance, different morphological associations and frequent co-infections. Vet J 250:1-5 (2019). PubMed: 31383413
    • Gang DG  et al. Sebaceous cell differentiation in a canine oral papilloma. J Vet Diagn Invest 30:569-571 (2018). PubMed: 29860939
    • Larmour LI  et al. A patient derived xenograft model of cervical cancer and cervical dysplasia. PLoS One 13:e0206539 (2018). PubMed: 30365542
    • Aksoy P & Meneses PI The Role of DCT in HPV16 Infection of HaCaTs. PLoS One 12:e0170158 (2017). WB . PubMed: 28095444
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-9 of 9 Abreviews or Q&A

    Question


    You could recommend me a secondary antibody for WB and ELISA against
    monoclonal mouse [BPV-1/1H8 + CAMVIR]ab2417, please.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 06 2013

    Answer

    I recommend ab97023, goat anti-mouse IgG H&L (heavy and light chains), assuming you want an HRP conjugate (horseradish peroxidase) for visualization. We also have fluorochrome conjugates and alkaline phosphatase conjugates, among others.

    Read More

    Abcam Scientific Support

    回复于 Feb 06 2013

    Western blot abreview for Anti-HPV antibody [BPV-1/1H8 + CAMVIR]

    Poor
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Tobacco Recombinant protein (Plant cell)
    Loading amount
    21 µg
    Specification
    Plant cell
    Gel Running Conditions
    Reduced Denaturing (gel 12%)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
    Read More

    Mr. Diego Grando Módolo

    Verified customer

    提交于 Dec 18 2012

    Question

    The formulation buffer for this product includes "Tissue Culture Supernatant". What are the constituents of this supernatant? Is 10% FBS or simiar included?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 06 2012

    Answer

    Thank you for contacting Abcam

    Theprotein carrier forHPV Cocktail Broad Spectrumis heat attenuated goat serum.

    Please let me know if there is anything else I can help you with.

    Read More

    Abcam Scientific Support

    回复于 Jun 06 2012

    Question

    Hi,

    I'm interested inantibody "ab30908".

    And I want to knowthis antibody can cross react withL1 protein of another type of HPV.

    If you don't have any information about cross reactivity,can you give mesome information about the sequence of immunogen?

    And I also want to know "ab2417" and "ab75574" can react withL1 protein of HPV.

    Can I use these antibodies in WB?

    Regards,

    Read More

    Abcam community

    Verified customer

    Asked on May 23 2012

    Answer

    Thank you for your enquiry and for your patience. It has taken some time to gather the information.

    Regardnig ab2417 Anti-HPV antibody [BPV-1/1H8 + CAMVIR], I can confirm thiswas raised against the major capsid protein L1. I have copied below some further information:

    The broad spectrum HPV antibody was produced (1H8) against SDS-disrupted bovine papillomavirus type 1 (BPV-1) and used to identify the product of the L1 open reading frame (ORF) of BPV-1. IH8 was found to be reactive with purified major capsid protein (MCP). The antibody was tested with ELISA and with an immunofluorescent technique and detected HPV-1, 6, 11, 16-16, 18, and 31 in formalin-fixed paraffin-embedded biopsy specimens. The CAMVIR-1 antibody was raised against the major capsid protein L1 of human papillomavirus type 16, using a recombinant vaccinia virus that expresses the L1 protein, as a target for screening. This antibody, designated CAMVIR-1, reacted with a 56 kilodalton protein in cells infected with L1-vaccinia virus, and the protein was present in HPV16. This antibody cocktail may also work in PAP smears, but has not been tested. A panel of p16 and Ki-67 can be used for further evaluation.

    Regarding ab30908HPV16 L1 antibody [289-16981], this should detectL1 as it was raised against HPV Type 16L1 protein.

    The ab75574 Anti-HPV antibody [K1H8],hasbeen sourced and tested externally. I am sorry we have not been able to obtain any further information yet for these antibodies, and therefore I would assume this has regrettably not been specifically tested for detection of L1.

    For western blotting, I can confirm that all tested applications covered by our guarantee are listed on the online datasheets which are updated as soon as we receive further information. I can confirm that ab2417 and ab30908 have been tested in WB. The ab75574 has not been tested in WB.

    I hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

    Read More

    Abcam Scientific Support

    回复于 May 23 2012

    Question

    Do you have a MSDS sheet for MsmAb to HPV(BPV-1-1H8)ab2417-250abcam?

    Read More

    Abcam community

    Verified customer

    Asked on May 21 2012

    Answer

    Thank you for contacting us.

    My colleague has created an SDS for ab2417 and it is now available for download from our website. I have also attached it to this email. Please let me know if you need any additional information.

    Read More

    Abcam Scientific Support

    回复于 May 21 2012

    Question

    I am getting some staining in cytoplasm and no staining in nuclear region.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 24 2011

    Answer

    Thanks you for your enquiry. Based on the following publications, there are cases where the HPV expression was found in the cytoplasm and nuclear region. 1) CASE REPORT: Corneal papilloma associated with papillomavirus in a one-humped camel (Camelus dromedarius). Veterinary Ophthalmology. Volume 13, Issue Supplement s1, pages 100–102, September 2010. (Fig 3) http://onlinelibrary.wiley.com/doi/10.1111/j.1463-5224.2010.00795.x/full 2) Clinical, pathological and immunohistochemical findings of bovine cutaneous papillomatosis. Ankara Üniv Vet Fak Derg, 58, 161-165, 2011 (Fig 2) http://dergiler.ankara.edu.tr/dergiler/11/1565/16994.pdf Therefore, the results you are seeing is not uncommon. Although on our datasheet it does state nuclear, this is information from SwissProt and may not be the case in an actual experiment. I hope this information will be helpful.

    Read More

    Abcam Scientific Support

    回复于 Nov 24 2011

    Question

    Anti-HPV antibody [BPV-1-1H8] (ab2417) I note from the web page [https://www.abcam.com/HPV-antibody-BPV-1-1H8-ab2417.html]that it states this is also an anti E6/early transforming protein E6 antibody, under the Alternative Names section. This cannot be true if it has been raised against whole (BPV-1) virus, because E6 is made only after infection and is not packaged within the virus. Anti-BPV L1 would be an alternative name. It would be worrying for someone to confuse E6 and L1 in either research or diagnostic uses of this antibody, so it might be worth correcting the mistake. The ELISA also confirms its reactivity with purified L1 (not E6).  Unless I am horribly confused.  We shall shortly be ordering this antibody to test it in various non-human species  Cheers!      

    Read More

    Abcam community

    Verified customer

    Asked on Nov 23 2011

    Answer

    Thank you for your enquiry regarding ab2417. After discussing your comments with some experts, we have reviewed the alternative names on the datasheet and amended it. We apologize for any inconvenience caused and thank you for bringing this matter to our attention. If you need any further assistance in the future, please do not hesitate to contact me.  

    Read More

    Abcam Scientific Support

    回复于 Nov 23 2011

    Question

    which dilution you suggest for IHC , paraffin sections for this antibody ? Thanks and regards

    Read More

    Abcam community

    Verified customer

    Asked on Jan 24 2011

    Answer

    Thank you for your enquiry. This antibody was used at a 1:80 dilution and the secondary antibody was a MACH3 Mouse AP Polymer. Please bear in mind that different samples and buffers used may require some optimization. I hope this information will be helpful. If there is anything else that I can help you with please do not hesitate to contact me. Have a nice day!

    Read More

    Abcam Scientific Support

    回复于 Jan 24 2011

    Question

    I'm writing to you for asking you a question and that is I'm interested in knowing an exact diluition for immunofluorescence for the antibody HPV (BPV-1-1H8) cod ab2417 that we're interested in ordering!

    Read More

    Abcam community

    Verified customer

    Asked on Oct 07 2010

    Answer

    The antibody was used at a concentration of 1:80 in IHC-P. Secondary was AP-polymer. We have not carried out ICC/IF for this antibody. Thus, we are unable to guarantee the suitability of this application. Probably antibody could be further diluted.

    Read More

    Abcam Scientific Support

    回复于 Oct 07 2010

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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