Anti-HOXA4抗体(ab131049)
Key features and details
- Rabbit polyclonal to HOXA4
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-HOXA4抗体
参阅全部 HOXA4 一抗 -
描述
兔多克隆抗体to HOXA4 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human HOXA4 aa 9-26 (C terminal).
Sequence:NSNYIEPKFPPFEEYAQH
Database link: Q00056 -
阳性对照
- WB: SW620 and PC12 cell lysates. ICC/IF: HeLa cells IHC-P: Human Mammary Cancer Tissue
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常规说明
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
Preservatives: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab131049 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 34 kDa. | |
| IHC-P | Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| ICC/IF | Use a concentration of 0.5 - 1 µg/ml. |
靶标
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功能
Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Binds to sites in the 5'-flanking sequence of its coding region with various affinities. The consensus sequences of the high and low affinity binding sites are 5'-TAATGA[CG]-3' and 5'-CTAATTTT-3'. -
组织特异性
Embryonic nervous system. -
序列相似性
Belongs to the Antp homeobox family. Deformed subfamily.
Contains 1 homeobox DNA-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 3201 Human
- Entrez Gene: 15401 Mouse
- Entrez Gene: 24453 Rat
- Omim: 142953 Human
- SwissProt: Q00056 Human
- SwissProt: P06798 Mouse
- SwissProt: P09635 Rat
- Unigene: 533357 Human
see all -
别名
- Dfd like protein antibody
- Homeo box A4 antibody
- Homeobox A4 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOXA4 antibody (ab131049)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Human Mammary Cancer Tissue using ab131049 at 1μg/ml.
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Immunocytochemistry/ Immunofluorescence - Anti-HOXA4 antibody (ab131049)Immunocytochemistry of HeLa cells using ab131049 at 1μg/ml.
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Western blot - Anti-HOXA4 antibody (ab131049)All lanes : Anti-HOXA4 antibody (ab131049) at 0.5 µg/ml
Lanes 1-2 : SW620 cells
Lane 3 : PC-12 cells
Predicted band size: 34 kDa
实验方案
文献 (2)
ab131049 被引用在 2 文献中.
- Kimura M et al. Homeobox A4 suppresses vascular remodeling by repressing YAP/TEAD transcriptional activity. EMBO Rep 21:e48389 (2020). PubMed: 32147946
- Morgan R et al. HOX transcription factors are potential targets and markers in malignant mesothelioma. BMC Cancer 16:85 (2016). PubMed: 26867567
