Anti-HOXA4抗体(ab131049)
Key features and details
- Rabbit polyclonal to HOXA4
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-HOXA4抗体
参阅全部 HOXA4 一抗 -
描述
兔多克隆抗体to HOXA4 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human HOXA4 aa 9-26 (C terminal).
Sequence:NSNYIEPKFPPFEEYAQH
Database link: Q00056 -
阳性对照
- WB: SW620 and PC12 cell lysates. ICC/IF: HeLa cells IHC-P: Human Mammary Cancer Tissue
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
Preservatives: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab131049 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 34 kDa. | |
| IHC-P | Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| ICC/IF | Use a concentration of 0.5 - 1 µg/ml. |
靶标
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功能
Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Binds to sites in the 5'-flanking sequence of its coding region with various affinities. The consensus sequences of the high and low affinity binding sites are 5'-TAATGA[CG]-3' and 5'-CTAATTTT-3'. -
组织特异性
Embryonic nervous system. -
序列相似性
Belongs to the Antp homeobox family. Deformed subfamily.
Contains 1 homeobox DNA-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 3201 Human
- Entrez Gene: 15401 Mouse
- Entrez Gene: 24453 Rat
- Omim: 142953 Human
- SwissProt: Q00056 Human
- SwissProt: P06798 Mouse
- SwissProt: P09635 Rat
- Unigene: 533357 Human
see all -
别名
- Dfd like protein antibody
- Homeo box A4 antibody
- Homeobox A4 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOXA4 antibody (ab131049)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Human Mammary Cancer Tissue using ab131049 at 1μg/ml.
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Immunocytochemistry/ Immunofluorescence - Anti-HOXA4 antibody (ab131049)Immunocytochemistry of HeLa cells using ab131049 at 1μg/ml.
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Western blot - Anti-HOXA4 antibody (ab131049)All lanes : Anti-HOXA4 antibody (ab131049) at 0.5 µg/ml
Lanes 1-2 : SW620 cells
Lane 3 : PC-12 cells
Predicted band size: 34 kDa
文献
ab131049 被引用在 1 文献中.
- Morgan R et al. HOX transcription factors are potential targets and markers in malignant mesothelioma. BMC Cancer 16:85 (2016). PubMed: 26867567
