重组Anti-Hormone sensitive lipase/HSL (phospho S853)抗体[EPR2329(2)] (ab109400)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2329(2)] to Hormone sensitive lipase/HSL (phospho S853)
- Suitable for: WB
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-Hormone sensitive lipase/HSL (phospho S853)抗体[EPR2329(2)]
参阅全部 Hormone sensitive lipase/HSL 一抗 -
描述
兔单克隆抗体[EPR2329(2)] to Hormone sensitive lipase/HSL (phospho S853) -
宿主
Rabbit -
特异性
The antibody only detects HSL phosphorylated on serine 853. -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Mouse, Human
预测可用于: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human skeletal muscle lysates and Mouse muscle lysates
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR2329(2) -
同种型
IgG -
研究领域
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109400于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/50000 - 1/200000. Detects a band of approximately 83 kDa (predicted molecular weight: 117 kDa).
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说明 |
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WB
1/50000 - 1/200000. Detects a band of approximately 83 kDa (predicted molecular weight: 117 kDa). |
靶标
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功能
In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. -
通路
Glycerolipid metabolism; triacylglycerol degradation. -
序列相似性
Belongs to the 'GDXG' lipolytic enzyme family. -
细胞定位
Cell membrane. Membrane > caveola. Cytoplasm > cytosol. Found in the high-density caveolae. Translocates to the cytoplasm from the caveolae upon insulin stimulation. - Information by UniProt
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数据库链接
- Entrez Gene: 3991 Human
- Entrez Gene: 16890 Mouse
- Entrez Gene: 25330 Rat
- Omim: 151750 Human
- SwissProt: Q05469 Human
- SwissProt: Q6NSL7 Human
- SwissProt: P54310 Mouse
- SwissProt: P15304 Rat
see all -
别名
- Hormone sensitive lipase antibody
- Hormone sensitive lipase testicular isoform antibody
- Hormone-sensitive lipase antibody
see all
图片
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All lanes : Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] (ab109400) at 1/50000 dilution
Lane 1 : Human skeletal muscle lysates
Lane 2 : Human skeletal muscle lysates treated with Lambda Phosphatase
Lysates/proteins at 10 µg per lane.
Predicted band size: 117 kDa
Observed band size: 83 kDa why is the actual band size different from the predicted? -
All lanes : Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] (ab109400) at 1/50000 dilution
Lane 1 : Mouse muscle lysate
Lane 2 : Mouse muscle lysate treated with Alkaline Phosphatase
Lysates/proteins at 10 µg per lane.
Predicted band size: 117 kDa
Observed band size: 83 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab109400 被引用在 6 文献中.
- Yang X et al. Targeting adipocytic discoidin domain receptor 2 impedes fat gain while increasing bone mass. Cell Death Differ 29:737-749 (2022). PubMed: 34645939
- Li J et al. Purinergic 2X7 receptor is involved in adipogenesis and lipid degradation. Exp Ther Med 23:81 (2022). PubMed: 34934450
- Zhao Y et al. Expression of Recombinant Rat Secretable FNDC5 in Pichia Pastoris and Detection of Its Biological Activity. Front Endocrinol (Lausanne) 13:852015 (2022). PubMed: 35321332
- Gao LM et al. Maternal pyrimidine nucleoside supplementation regulates fatty acid, amino acid and glucose metabolism of neonatal piglets. Anim Nutr 11:309-321 (2022). PubMed: 36312745
- Wu W et al. MicroRNA-29b/29c targeting CTRP6 influences porcine adipogenesis via the AKT/PKA/MAPK Signalling pathway. Adipocyte 10:264-274 (2021). PubMed: 33938394
- Feng J et al. IL-25 stimulates M2 macrophage polarization and thereby promotes mitochondrial respiratory capacity and lipolysis in adipose tissues against obesity. Cell Mol Immunol 15:493-505 (2018). PubMed: 28194019