Anti-hnRNP L抗体[4D11] (ab6106)

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Western blot - Anti-hnRNP L antibody [4D11] (ab6106)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP L antibody [4D11] (ab6106)
  • Immunoprecipitation - Anti-hnRNP L antibody [4D11] (ab6106)
  • Flow Cytometry - Anti-hnRNP L antibody [4D11] (ab6106)

Key features and details

  • Mouse monoclonal [4D11] to hnRNP L
  • Suitable for: IHC-P, WB, IP, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-hnRNP L抗体[4D11]
    参阅全部 hnRNP L 一抗

  • 描述

    小鼠单克隆抗体[4D11] to hnRNP L

  • 宿主

    Mouse

  • 经测试应用

    适用于: IHC-P, WB, IP, Flow Cytmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Human hnRNP proteins (from HeLa cells) purified by affinity chromatography on ssDNA agarose.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6106于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB (2)
1/2000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
IP (1)
Use at an assay dependent concentration.
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
说明
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/2000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
IP
Use at an assay dependent concentration.
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

靶标

  • 功能

    This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Is associated with most nascent transcripts including those of the landmark giant loops of amphibian lampbrush chromosomes. Associates, together with APEX1, to the negative calcium responsive element (nCaRE) B2 of the APEX2 promoter.

  • 序列相似性

    Contains 3 RRM (RNA recognition motif) domains.

  • 翻译后修饰

    Several isoelectric forms of the L protein are probably the results of post-translational modifications.

  • 细胞定位

    Nucleus > nucleoplasm. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt

  • 数据库链接

    • 别名

      • D830027H13Rik antibody
      • FLJ35509 antibody
      • Heterogeneous nuclear ribonucleoprotein L antibody
      • hnRNP L antibody
      • hnRNP-L antibody
      • Hnrnpl antibody
      • hnRPL antibody
      • HNRPL_HUMAN antibody
      • P/OKcl.14 antibody
      see all

    图片

    • Western blot - Anti-hnRNP L antibody [4D11] (ab6106)
      Western blot - Anti-hnRNP L antibody [4D11] (ab6106)
      All lanes : Anti-hnRNP L antibody [4D11] (ab6106) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
      Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 64 kDa
      Observed band size: 64 kDa
      Additional bands at: 60 kDa, 68 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 1 minute
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP L antibody [4D11] (ab6106)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP L antibody [4D11] (ab6106)
      IHC image of ab6106 staining in human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6106, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Immunoprecipitation - Anti-hnRNP L antibody [4D11] (ab6106)
      Immunoprecipitation - Anti-hnRNP L antibody [4D11] (ab6106)
      hnRNP L was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Mouse monoclonal to hnRNP L (ab6106) and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
      The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
      Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab6106.
      Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
      Band: 65kDa: hnRNP L. Non specific - 70kDa and 55kDa: We are unsure as to the identity of this extra band.
    • Flow Cytometry - Anti-hnRNP L antibody [4D11] (ab6106)
      Flow Cytometry - Anti-hnRNP L antibody [4D11] (ab6106)
      Overlay histogram showing Jurkat cells stained with ab6106 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6106, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    文献 (39)

    发表研究结果有使用 ab6106？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab6106 被引用在 39 文献中.

    • Van Nostrand EL  et al. Principles of RNA processing from analysis of enhanced CLIP maps for 150 RNA binding proteins. Genome Biol 21:90 (2020). PubMed: 32252787
    • Mallory MJ  et al. Reciprocal regulation of hnRNP C and CELF2 through translation and transcription tunes splicing activity in T cells. Nucleic Acids Res 48:5710-5719 (2020). PubMed: 32338744
    • Zhao L  et al. A Long Non-coding RNA IVRPIE Promotes Host Antiviral Immune Responses Through Regulating Interferon ß1 and ISG Expression. Front Microbiol 11:260 (2020). PubMed: 32153544
    • Ji J  et al. Long Noncoding RNA SChLAP1 Forms a Growth-Promoting Complex with HNRNPL in Human Glioblastoma through Stabilization of ACTN4 and Activation of NF-?B Signaling. Clin Cancer Res 25:6868-6881 (2019). PubMed: 31492748
    • Kishor A  et al. hnRNP L-dependent protection of normal mRNAs from NMD subverts quality control in B cell lymphoma. EMBO J 38:N/A (2019). PubMed: 30530525
    View all Publications for this product

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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-hnRNP L antibody [4D11]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Lymphocytes)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Loading amount
    30 µg
    Specification
    Lymphocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Mar 25 2017

    Western blot abreview for Anti-hnRNP L antibody [4D11]

     Good
    Abreviews
    Application
    Western blot
    Sample
    Rat Cell lysate - other (rat oligodendrocytes nuclear or cytoplasmic)
    Specification
    rat oligodendrocytes nuclear or cytoplasmic
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More

    Abcam user community

    Verified customer

    提交于 Nov 28 2005

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