Key features and details
- Mouse monoclonal [4D11] to hnRNP L
- Suitable for: IHC-P, WB, IP, Flow Cyt
- Reacts with: Mouse, Rat, Human, Newt
- Isotype: IgG1
参阅全部 hnRNP L 一抗
描述小鼠单克隆抗体[4D11] to hnRNP L
经测试应用适用于: IHC-P, WB, IP, Flow Cytmore details
种属反应性与反应: Mouse, Rat, Human, Newt
Human hnRNP proteins (from HeLa cells) purified by affinity chromatography on ssDNA agarose.
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.1% Sodium azide
Concentration information loading...
纯度Protein A purified
纯化说明Purified from supernatant.
Our Abpromise guarantee covers the use of ab6106 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).|
|IP||Use at an assay dependent concentration.|
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
功能This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Is associated with most nascent transcripts including those of the landmark giant loops of amphibian lampbrush chromosomes. Associates, together with APEX1, to the negative calcium responsive element (nCaRE) B2 of the APEX2 promoter.
序列相似性Contains 3 RRM (RNA recognition motif) domains.
翻译后修饰Several isoelectric forms of the L protein are probably the results of post-translational modifications.
细胞定位Nucleus > nucleoplasm. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
- Information by UniProt
- D830027H13Rik antibody
- FLJ35509 antibody
- Heterogeneous nuclear ribonucleoprotein L antibody
All lanes : Anti-hnRNP L antibody [4D11] (ab6106) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 64 kDa
Observed band size: 64 kDa
Additional bands at: 60 kDa, 68 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
IHC image of ab6106 staining in human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6106, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
hnRNP L was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Mouse monoclonal to hnRNP L (ab6106) and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab6106.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
Band: 65kDa: hnRNP L. Non specific - 70kDa and 55kDa: We are unsure as to the identity of this extra band.
Overlay histogram showing Jurkat cells stained with ab6106 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6106, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
ab6106 被引用在 36 文献中.
- Van Nostrand EL et al. Principles of RNA processing from analysis of enhanced CLIP maps for 150 RNA binding proteins. Genome Biol 21:90 (2020). PubMed: 32252787
- Mallory MJ et al. Reciprocal regulation of hnRNP C and CELF2 through translation and transcription tunes splicing activity in T cells. Nucleic Acids Res 48:5710-5719 (2020). PubMed: 32338744
- Zhao L et al. A Long Non-coding RNA IVRPIE Promotes Host Antiviral Immune Responses Through Regulating Interferon ß1 and ISG Expression. Front Microbiol 11:260 (2020). PubMed: 32153544
- Ji J et al. Long Noncoding RNA SChLAP1 Forms a Growth-Promoting Complex with HNRNPL in Human Glioblastoma through Stabilization of ACTN4 and Activation of NF-?B Signaling. Clin Cancer Res 25:6868-6881 (2019). PubMed: 31492748
- Majewski L et al. Myosin VI in the nucleus of neurosecretory PC12 cells: Stimulation-dependent nuclear translocation and interaction with nuclear proteins. Nucleus 9:125-141 (2018). PubMed: 29293066