Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Loading amount
30 µg
Specification
HeLa
Gel Running Conditions
Reduced Denaturing (12.5%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk
Secondary antibody
Name
Non-Abcam antibody was used: Polyclonal Goat Anti-Mouse Immunoglobulins/HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
ECL+
Exposure
30 second(s)
Bands
Specific: 34 kDa
Additional data
Additional Notes
Lanes 1-2 hnRNPA2 shRNA-1
Lanes 3-4 hnRNPA2 shRNA-2
Lanes 5-6 Control shRNA
Lane 7 Untreated HeLa
Lanes 3-4 hnRNPA2 shRNA-2
Lanes 5-6 Control shRNA
Lane 7 Untreated HeLa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Nov 26 2009