Application
Western blot
Sample
Human Cell lysate - whole cell (HeLA)
Loading amount
25 µg
Specification
HeLA
Gel Running Conditions
Reduced Denaturing (17.5)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/1000
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: Li-COR Goat anti rabbit secondary
Host species: Goat
Clonality: Monoclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Monoclonal
Conjugation: IRDye® 800CW
Dilution
1/1000
Detection
Detection method
Li-COR odyssey scanner
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 18 kDa
Additional data
Additional Notes
Note: HMGA2 is 12 kD on Swissprot, but the WB generated clear band at 18kDa which matches with datasheet
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Feb 01 2012