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Microbiology Interspecies Interaction Host Virus Interaction

Anti-HMGA1a / HMGA1b抗体- ChIP Grade (ab4078)

  • Datasheet
Reviews (2)Q&A (1)References (22)

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Western blot - Anti-HMGA1a / HMGA1b antibody - ChIP Grade (ab4078)
  • Immunocytochemistry/ Immunofluorescence - Anti-HMGA1a / HMGA1b antibody - ChIP Grade (ab4078)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA1a / HMGA1b antibody - ChIP Grade (ab4078)

Key features and details

  • Rabbit polyclonal to HMGA1a / HMGA1b - ChIP Grade
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-HMGA1a / HMGA1b抗体- ChIP Grade
    参阅全部 HMGA1a / HMGA1b 一抗
  • 描述

    兔多克隆抗体to HMGA1a / HMGA1b - ChIP Grade
  • 宿主

    Rabbit
  • 特异性

    This antibody detects, by Western blot on human GFP fusion proteins in cell lysate, both HMGA1a and the splice variant HMGA1b, but does not detect HMGA2 (see review and Western blot picture). The antibody has not yet been successfully used to detect endogenous protein.
  • 经测试应用

    适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Zebrafish, Neurospora crassa, Chinese hamster
  • 免疫原

    Synthetic peptide corresponding to Human HMGA1a/ HMGA1b aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab20073)

  • 阳性对照

    • This antibody gave a positive result in human placenta FFPE tissue sections
  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Epigenetics and Nuclear Signaling
    • Histones
    • HMGs
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HMG Box
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Other
    • Epigenetics and Nuclear Signaling
    • ChIP assays
    • ChIP antibodies

相关产品

  • ChIP Related Products

    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human HMGA1a / HMGA1b protein (ab109927)

应用

Our Abpromise guarantee covers the use of ab4078 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11.5 kDa).
IHC-P Use at an assay dependent concentration.
ICC/IF 1/250 - 1/500.

Although this antibody gives a specific signal in Immunofluoresence, we have had suggestions that strong background signals are seen. See review by Robert Hock and IF picture below.

靶标

  • 功能

    HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions.
  • 疾病相关

    Note=A chromosomal aberration involving HMGA1 is found in pulmonary chondroid hamartoma. Translocation t(6;14)(p21;q23-24) with RAD51L1.
  • 序列相似性

    Belongs to the HMGA family.
    Contains 3 A.T hook DNA-binding domains.
  • 翻译后修饰

    Constitutively phosphorylated on two or three sites. Phosphorylated upon DNA damage, probably by ATM or ATR. Hyperphosphorylated at early stages of apoptosis, followed by dephosphorylation and methylation, which coincides with chromatin condensation. Isoform HMG-Y can be phosphorylated by HIPK2.
    HMG-Y is not methylated.
    Methylation at Arg-58 is mutually exclusive with methylation at Arg-60.
  • 细胞定位

    Nucleus. Chromosome.
  • Target information above from: UniProt accession P17096 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 3159 Human
    • Entrez Gene: 111241 Mouse
    • Entrez Gene: 15361 Mouse
    • Entrez Gene: 117062 Rat
    • Omim: 600701 Human
    • SwissProt: Q9QXP3 Chinese hamster
    • SwissProt: P17096 Human
    • SwissProt: P17095 Mouse
    • SwissProt: Q8K585 Rat
    • Unigene: 518805 Human
    • Unigene: 703764 Human
    • Unigene: 426212 Mouse
    • Unigene: 440289 Mouse
    • Unigene: 4438 Mouse
    • Unigene: 469945 Mouse
    • Unigene: 83614 Rat
    see all
  • 别名

    • High mobility group AT hook 1 antibody
    • High mobility group AT-hook protein 1 antibody
    • High mobility group protein A1 antibody
    • High mobility group protein HMG-I/HMG-Y antibody
    • High mobility group protein R antibody
    • HMG-I(Y) antibody
    • HMGA 1a antibody
    • HMGA 1b antibody
    • HMGA1 antibody
    • HMGA1_HUMAN antibody
    • HMGI antibody
    • HMGI/Y antibody
    • HMGR antibody
    • HMGY antibody
    see all

图片

  • Western blot - Anti-HMGA1a / HMGA1b antibody - ChIP Grade (ab4078)
    Western blot - Anti-HMGA1a / HMGA1b antibody - ChIP Grade (ab4078)This image is courtesy of Monika Harrer
    Lanes 1-4 : Anti-HMGA1a / HMGA1b antibody - ChIP Grade (ab4078) at 1/1000 dilution
    Lanes 5-8 : Anti-GFP

    Lane 1 : Whole cell lysate from HepG2 cells transfected with plasmids coding for hHMGA1a GFP fusion proteins.
    Lanes 2 & 6 : Whole cell lysate from HepG2 cells transfected with plasmids coding for hHMGA1b-GFP fusion proteins
    Lanes 3 & 7 : Whole cell lysate from HepG2 cells transfected with plasmids coding for hHMGA2-GFP fusion proteins
    Lane 4 : hole cell lysate from HepG2 cells transfected with plasmids coding for NLS-GFP fusion proteins
    Lane 5 : Whole cell lysate from HepG2 cells transfected with plasmids coding for hHMGA1a-GFP fusion proteins
    Lane 8 : Whole cell lysate from HepG2 cells transfected with plasmids coding for NLS-GFP fusion proteins

    Secondary
    All lanes : Anti-rabbit HRP at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 11.5 kDa



    Western blot using ab4078.

    Whole cell lysate from HepG2 cells transfected with plasmids coding for hHMGA1a-, hHMGA1b-, hHMGA2- or NLS- (nuclear localisation signal) GFP fusion proteins.

    ab4078 was diluted 1/1000 in 5% milk/TBS and incubated for 1 hr at room temperature. Secondary HRP anti-rabbit antibody was diluted 1/10000 in 5% milk/TBS and incubated for 1 hr at room temperature.

    Lane 1: hHMGA1a-GFP (ab4078)
    Lane 2: hHMGA1b-GFP (ab4078)
    Lane 3: hHMGA2-GFP (ab4078)
    Lane 4: NLS-GFP (ab4078)
    Lane 5: hHMGA1a-GFP (anti-GFP)
    Lane 6: hHMGA1b-GFP (anti-GFP)
    Lane 7: hHMGA2-GFP (anti-GFP)
    Lane 8: NLS-GFP (anti-GFP)

    The detected size is that expected for the fusion of an 11kD and 26kD protein (GFP).

  • Immunocytochemistry/ Immunofluorescence - Anti-HMGA1a / HMGA1b antibody - ChIP Grade (ab4078)
    Immunocytochemistry/ Immunofluorescence - Anti-HMGA1a / HMGA1b antibody - ChIP Grade (ab4078)This image is courtesy of Robert Hock

    Immunofluoresence on MCF-7 cells transfected with human HMGA1a-GFP using ab4078.

    A: Hoechst stain
    B: Phasecontrast
    C: TexasRed HMG-I/HMG-Y (ab4078)
    D: HMGA1a-GFP
    E: Merge

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA1a / HMGA1b antibody - ChIP Grade (ab4078)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA1a / HMGA1b antibody - ChIP Grade (ab4078)
    IHC image of ab4078 staining in human placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4078, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

实验方案

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

    • Datasheet
  • 文献 (22)

    发表研究结果有使用 ab4078?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab4078 被引用在 22 文献中.

    • Teng K  et al. KIFC1 is activated by TCF-4 and promotes hepatocellular carcinoma pathogenesis by regulating HMGA1 transcriptional activity. J Exp Clin Cancer Res 38:329 (2019). PubMed: 31340839
    • Tolza C  et al. AP-1 Signaling by Fra-1 Directly Regulates HMGA1 Oncogene Transcription in Triple-Negative Breast Cancers. Mol Cancer Res 17:1999-2014 (2019). PubMed: 31300541
    • Patel ZH  et al. A plausibly causal functional lupus-associated risk variant in the STAT1-STAT4 locus. Hum Mol Genet 27:2392-2404 (2018). PubMed: 29912393
    • Zhang H  et al. Mandatory role of HMGA1 in human airway epithelial normal differentiation and post-injury regeneration. Oncotarget 9:14324-14337 (2018). PubMed: 29581847
    • Parry AJ  et al. NOTCH-mediated non-cell autonomous regulation of chromatin structure during senescence. Nat Commun 9:1840 (2018). PubMed: 29743479
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-3 of 3 Abreviews or Q&A

    Question

    The most recent vial of ab4078, rabbit polyclonal to HMGA1a / HMGA1b (order 911447) failed in ChIP twice. Previous vials from othere lots have worked well with the same protocol and samples.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 14 2011

    Answer

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement (from a different lot) with the order number 949551. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

    Read More

    Abcam Scientific Support

    回复于 Sep 14 2011

    Western blot abreview for Anti-HMGA1a / HMGA1b antibody - ChIP Grade

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Zebrafish Tissue lysate - whole (1.5 zebrafish embryo (74hpf)/lane)
    Specification
    1.5 zebrafish embryo (74hpf)/lane
    Gel Running Conditions
    Reduced Denaturing (13%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 20 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Dr. Jan Brocher

    Verified customer

    提交于 Apr 20 2010

    ChIP abreview for Anti-HMGA1a / HMGA1b antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Human Cell lysate - nuclear (Human embryonic stem cells)
    Specification
    Human embryonic stem cells
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 15 minute(s) and 0 second(s)
    Specification of the cross-linking agent: formaldehyde
    Detection step
    Real-time PCR
    Positive control
    Histone H3 antibody from Abcam
    Negative control
    Rabbit IgG
    Read More

    Abcam user community

    Verified customer

    提交于 Dec 15 2009

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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