Anti-HMGA1a / HMGA1b抗体- ChIP Grade (ab4078)
Key features and details
- Rabbit polyclonal to HMGA1a / HMGA1b - ChIP Grade
- Suitable for: WB, ChIP, IHC-P, ICC/IF
- Reacts with: Human, Zebrafish
- Isotype: IgG
概述
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产品名称
Anti-HMGA1a / HMGA1b抗体- ChIP Grade
参阅全部 HMGA1a / HMGA1b 一抗 -
描述
兔多克隆抗体to HMGA1a / HMGA1b - ChIP Grade -
宿主
Rabbit -
特异性
This antibody detects, by Western blot on human GFP fusion proteins in cell lysate, both HMGA1a and the splice variant HMGA1b, but does not detect HMGA2 (see review and Western blot picture). The antibody has not yet been successfully used to detect endogenous protein. -
经测试应用
适用于: WB, ChIP, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human, Zebrafish
预测可用于: Mouse, Rat, Chicken, Neurospora crassa, Chinese hamster
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免疫原
Synthetic peptide corresponding to Human HMGA1a/ HMGA1b aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab20073) -
阳性对照
- This antibody gave a positive result in human placenta FFPE tissue sections
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab4078 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11.5 kDa). | |
| ChIP | Use at an assay dependent concentration. See Abreview submitted on 15 December 2009. |
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| IHC-P | Use at an assay dependent concentration. | |
| ICC/IF | 1/250 - 1/500. Although this antibody gives a specific signal in Immunofluoresence, we have had suggestions that strong background signals are seen. See review by Robert Hock and IF picture below. |
靶标
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功能
HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions. -
疾病相关
Note=A chromosomal aberration involving HMGA1 is found in pulmonary chondroid hamartoma. Translocation t(6;14)(p21;q23-24) with RAD51L1. -
序列相似性
Belongs to the HMGA family.
Contains 3 A.T hook DNA-binding domains. -
翻译后修饰
Constitutively phosphorylated on two or three sites. Phosphorylated upon DNA damage, probably by ATM or ATR. Hyperphosphorylated at early stages of apoptosis, followed by dephosphorylation and methylation, which coincides with chromatin condensation. Isoform HMG-Y can be phosphorylated by HIPK2.
HMG-Y is not methylated.
Methylation at Arg-58 is mutually exclusive with methylation at Arg-60. -
细胞定位
Nucleus. Chromosome. - Information by UniProt
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数据库链接
- Entrez Gene: 3159 Human
- Entrez Gene: 111241 Mouse
- Entrez Gene: 15361 Mouse
- Entrez Gene: 117062 Rat
- Omim: 600701 Human
- SwissProt: Q9QXP3 Chinese hamster
- SwissProt: P17096 Human
- SwissProt: P17095 Mouse
see all -
别名
- High mobility group AT hook 1 antibody
- High mobility group AT-hook protein 1 antibody
- High mobility group protein A1 antibody
see all
图片
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Western blot - Anti-HMGA1a / HMGA1b antibody - ChIP Grade (ab4078)This image is courtesy of Monika HarrerLanes 1-4 : Anti-HMGA1a / HMGA1b antibody - ChIP Grade (ab4078) at 1/1000 dilution
Lanes 5-8 : Anti-GFP
Lane 1 : Whole cell lysate from HepG2 cells transfected with plasmids coding for hHMGA1a GFP fusion proteins.
Lanes 2 & 6 : Whole cell lysate from HepG2 cells transfected with plasmids coding for hHMGA1b-GFP fusion proteins
Lanes 3 & 7 : Whole cell lysate from HepG2 cells transfected with plasmids coding for hHMGA2-GFP fusion proteins
Lane 4 : hole cell lysate from HepG2 cells transfected with plasmids coding for NLS-GFP fusion proteins
Lane 5 : Whole cell lysate from HepG2 cells transfected with plasmids coding for hHMGA1a-GFP fusion proteins
Lane 8 : Whole cell lysate from HepG2 cells transfected with plasmids coding for NLS-GFP fusion proteins
Secondary
All lanes : Anti-rabbit HRP at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 11.5 kDaWestern blot using ab4078.
Whole cell lysate from HepG2 cells transfected with plasmids coding for hHMGA1a-, hHMGA1b-, hHMGA2- or NLS- (nuclear localisation signal) GFP fusion proteins.
ab4078 was diluted 1/1000 in 5% milk/TBS and incubated for 1 hr at room temperature. Secondary HRP anti-rabbit antibody was diluted 1/10000 in 5% milk/TBS and incubated for 1 hr at room temperature.
Lane 1: hHMGA1a-GFP (ab4078)
Lane 2: hHMGA1b-GFP (ab4078)
Lane 3: hHMGA2-GFP (ab4078)
Lane 4: NLS-GFP (ab4078)
Lane 5: hHMGA1a-GFP (anti-GFP)
Lane 6: hHMGA1b-GFP (anti-GFP)
Lane 7: hHMGA2-GFP (anti-GFP)
Lane 8: NLS-GFP (anti-GFP)The detected size is that expected for the fusion of an 11kD and 26kD protein (GFP).
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Immunocytochemistry/ Immunofluorescence - Anti-HMGA1a / HMGA1b antibody - ChIP Grade (ab4078)This image is courtesy of Robert HockImmunofluoresence on MCF-7 cells transfected with human HMGA1a-GFP using ab4078.
A: Hoechst stain
B: Phasecontrast
C: TexasRed HMG-I/HMG-Y (ab4078)
D: HMGA1a-GFP
E: Merge -
ChIP - Anti-HMGA1a / HMGA1b antibody - ChIP Grade (ab4078)This image is courtesy of an anonymous AbreviewChromatin was prepared from the Human Embryonic Stem Cells. The cross-linking (X-ChiP) technique was used; crosslinking was performed for 15 minutes in formaldehyde. The primary antibody was diluted 1/100 in IP dilution buffer and incubated with the sample for 12 hours at 4°C. Histone H3 was used as the positive control, whilst normal rabbit IgG was used as the negative control. The immunoprecipitated DNA was quantified by real time PCR. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA1a / HMGA1b antibody - ChIP Grade (ab4078)IHC image of ab4078 staining in human placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4078, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
数据表及文件
文献 (21)
ab4078 被引用在 21 文献中.
- Teng K et al. KIFC1 is activated by TCF-4 and promotes hepatocellular carcinoma pathogenesis by regulating HMGA1 transcriptional activity. J Exp Clin Cancer Res 38:329 (2019). PubMed: 31340839
- Patel ZH et al. A plausibly causal functional lupus-associated risk variant in the STAT1-STAT4 locus. Hum Mol Genet 27:2392-2404 (2018). PubMed: 29912393
- Zhang H et al. Mandatory role of HMGA1 in human airway epithelial normal differentiation and post-injury regeneration. Oncotarget 9:14324-14337 (2018). PubMed: 29581847
- Parry AJ et al. NOTCH-mediated non-cell autonomous regulation of chromatin structure during senescence. Nat Commun 9:1840 (2018). PubMed: 29743479
- Fu F et al. HMGA1 exacerbates tumor growth through regulating the cell cycle and accelerates migration/invasion via targeting miR-221/222 in cervical cancer. Cell Death Dis 9:594 (2018). PubMed: 29789601
