Anti-HMGA1a / HMGA1b抗体(ab4078)

Reviews (2)Q&A (1)References (24)
Western blot - Anti-HMGA1a / HMGA1b antibody (ab4078)
  • Immunocytochemistry/ Immunofluorescence - Anti-HMGA1a / HMGA1b antibody (ab4078)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA1a / HMGA1b antibody (ab4078)

Key features and details

  • Rabbit polyclonal to HMGA1a / HMGA1b
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-HMGA1 antibody [EPR22421-101] - ChIP Grade (ab252930)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术，可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-HMGA1a / HMGA1b抗体

  • 描述

    兔多克隆抗体to HMGA1a / HMGA1b

  • 宿主

    Rabbit

  • 特异性

    This antibody detects, by Western blot on human GFP fusion proteins in cell lysate, both HMGA1a and the splice variant HMGA1b, but does not detect HMGA2 (see review and Western blot picture). The antibody has not yet been successfully used to detect endogenous protein.

  • 经测试应用

    适用于: WB, IHC-P, ICC/IFmore details

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Zebrafish, Neurospora crassa, Chinese hamster

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • This antibody gave a positive result in human placenta FFPE tissue sections

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab4078于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11.5 kDa).
IHC-P
Use at an assay dependent concentration.
ICC/IF
1/250 - 1/500.

Although this antibody gives a specific signal in Immunofluoresence, we have had suggestions that strong background signals are seen. See review by Robert Hock and IF picture below.
说明
WB
1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11.5 kDa).
IHC-P
Use at an assay dependent concentration.
ICC/IF
1/250 - 1/500.

Although this antibody gives a specific signal in Immunofluoresence, we have had suggestions that strong background signals are seen. See review by Robert Hock and IF picture below.

靶标

  • 功能

    HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions.

  • 疾病相关

    Note=A chromosomal aberration involving HMGA1 is found in pulmonary chondroid hamartoma. Translocation t(6;14)(p21;q23-24) with RAD51L1.

  • 序列相似性

    Belongs to the HMGA family.
    Contains 3 A.T hook DNA-binding domains.

  • 翻译后修饰

    Constitutively phosphorylated on two or three sites. Phosphorylated upon DNA damage, probably by ATM or ATR. Hyperphosphorylated at early stages of apoptosis, followed by dephosphorylation and methylation, which coincides with chromatin condensation. Isoform HMG-Y can be phosphorylated by HIPK2.
    HMG-Y is not methylated.
    Methylation at Arg-58 is mutually exclusive with methylation at Arg-60.

  • 细胞定位

    Nucleus. Chromosome.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • High mobility group AT hook 1 antibody
    • High mobility group AT-hook protein 1 antibody
    • High mobility group protein A1 antibody
    • High mobility group protein HMG-I/HMG-Y antibody
    • High mobility group protein R antibody
    • HMG-I(Y) antibody
    • HMGA 1a antibody
    • HMGA 1b antibody
    • HMGA1 antibody
    • HMGA1_HUMAN antibody
    • HMGI antibody
    • HMGI/Y antibody
    • HMGR antibody
    • HMGY antibody
    see all

图片

  • Western blot - Anti-HMGA1a / HMGA1b antibody (ab4078)
    Western blot - Anti-HMGA1a / HMGA1b antibody (ab4078)This image is courtesy of Monika Harrer
    Lanes 1-4 : Anti-HMGA1a / HMGA1b antibody (ab4078) at 1/1000 dilution
    Lanes 5-8 : Anti-GFP

    Lane 1 : Whole cell lysate from HepG2 cells transfected with plasmids coding for hHMGA1a GFP fusion proteins.
    Lanes 2 & 6 : Whole cell lysate from HepG2 cells transfected with plasmids coding for hHMGA1b-GFP fusion proteins
    Lanes 3 & 7 : Whole cell lysate from HepG2 cells transfected with plasmids coding for hHMGA2-GFP fusion proteins
    Lane 4 : hole cell lysate from HepG2 cells transfected with plasmids coding for NLS-GFP fusion proteins
    Lane 5 : Whole cell lysate from HepG2 cells transfected with plasmids coding for hHMGA1a-GFP fusion proteins
    Lane 8 : Whole cell lysate from HepG2 cells transfected with plasmids coding for NLS-GFP fusion proteins

    Secondary
    All lanes : Anti-rabbit HRP at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 11.5 kDa



    Western blot using ab4078.

    Whole cell lysate from HepG2 cells transfected with plasmids coding for hHMGA1a-, hHMGA1b-, hHMGA2- or NLS- (nuclear localisation signal) GFP fusion proteins.

    ab4078 was diluted 1/1000 in 5% milk/TBS and incubated for 1 hr at room temperature. Secondary HRP anti-rabbit antibody was diluted 1/10000 in 5% milk/TBS and incubated for 1 hr at room temperature.

    Lane 1: hHMGA1a-GFP (ab4078)
    Lane 2: hHMGA1b-GFP (ab4078)
    Lane 3: hHMGA2-GFP (ab4078)
    Lane 4: NLS-GFP (ab4078)
    Lane 5: hHMGA1a-GFP (anti-GFP)
    Lane 6: hHMGA1b-GFP (anti-GFP)
    Lane 7: hHMGA2-GFP (anti-GFP)
    Lane 8: NLS-GFP (anti-GFP)

    The detected size is that expected for the fusion of an 11kD and 26kD protein (GFP).

  • Immunocytochemistry/ Immunofluorescence - Anti-HMGA1a / HMGA1b antibody (ab4078)
    Immunocytochemistry/ Immunofluorescence - Anti-HMGA1a / HMGA1b antibody (ab4078)This image is courtesy of Robert Hock

    Immunofluoresence on MCF-7 cells transfected with human HMGA1a-GFP using ab4078.

    A: Hoechst stain
    B: Phasecontrast
    C: TexasRed HMG-I/HMG-Y (ab4078)
    D: HMGA1a-GFP
    E: Merge

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA1a / HMGA1b antibody (ab4078)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA1a / HMGA1b antibody (ab4078)
    IHC image of ab4078 staining in human placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4078, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

文献 (24)

发表研究结果有使用 ab4078？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab4078 被引用在 24 文献中.

  • Gorbounov M  et al. High mobility group A1 (HMGA1) protein and gene expression correlate with ER-negativity and poor outcomes in breast cancer. Breast Cancer Res Treat 179:25-35 (2020). PubMed: 31531802
  • Wei S  et al. KIF2C: a novel link between Wnt/ß-catenin and mTORC1 signaling in the pathogenesis of hepatocellular carcinoma. Protein Cell N/A:N/A (2020). PubMed: 32748349
  • Teng K  et al. KIFC1 is activated by TCF-4 and promotes hepatocellular carcinoma pathogenesis by regulating HMGA1 transcriptional activity. J Exp Clin Cancer Res 38:329 (2019). PubMed: 31340839
  • Tolza C  et al. AP-1 Signaling by Fra-1 Directly Regulates HMGA1 Oncogene Transcription in Triple-Negative Breast Cancers. Mol Cancer Res 17:1999-2014 (2019). PubMed: 31300541
  • Patel ZH  et al. A plausibly causal functional lupus-associated risk variant in the STAT1-STAT4 locus. Hum Mol Genet 27:2392-2404 (2018). PubMed: 29912393
View all Publications for this product

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1-3 of 3 Abreviews or Q&A

Western blot abreview for Anti-HMGA1a / HMGA1b antibody - ChIP Grade

 Average
Abreviews
abreview image
Application
Western blot
Sample
Zebrafish Tissue lysate - whole (1.5 zebrafish embryo (74hpf)/lane)
Specification
1.5 zebrafish embryo (74hpf)/lane
Gel Running Conditions
Reduced Denaturing (13%)
Blocking step
Milk as blocking agent for 1 hour(s) and 20 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Dr. Jan Brocher

Verified customer

提交于 Apr 20 2010

ChIP abreview for Anti-HMGA1a / HMGA1b antibody - ChIP Grade

 Excellent
Abreviews
abreview image
Application
ChIP
Sample
Human Cell lysate - nuclear (Human embryonic stem cells)
Specification
Human embryonic stem cells
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde
Detection step
Real-time PCR
Positive control
Histone H3 antibody from Abcam
Negative control
Rabbit IgG
Read More

Abcam user community

Verified customer

提交于 Dec 15 2009

Question

The most recent vial of ab4078, rabbit polyclonal to HMGA1a / HMGA1b (order 911447) failed in ChIP twice. Previous vials from othere lots have worked well with the same protocol and samples.

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Answer

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement (from a different lot) with the order number 949551. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

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