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Anti-HLA G抗体[MEM-G/1] (ab7759)

Reviews (3)Q&A (10)References (21)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA G antibody [MEM-G/1] (ab7759)
  • Western blot - Anti-HLA G antibody [MEM-G/1] (ab7759)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA G antibody [MEM-G/1] (ab7759)
  • Western blot - Anti-HLA G antibody [MEM-G/1] (ab7759)

Key features and details

  • Mouse monoclonal [MEM-G/1] to HLA G
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

Biotin

概述

  • 产品名称

    Anti-HLA G抗体[MEM-G/1]
    参阅全部 HLA G 一抗

  • 描述

    小鼠单克隆抗体[MEM-G/1] to HLA G

  • 宿主

    Mouse

  • 特异性

    The antibody reacts with denaturated HLA-G heavy chain.

  • 经测试应用

    适用于: IHC-P, WBmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant full length denatured heavy chain (Human).

  • 阳性对照

    • IHC-P: First trimester placenta and pulmonary diseases. WB: ab29745; HeLa and JEG-3 cell lysates.

  • 常规说明

    This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 纯化说明

    Purified from TCS. Purity >95% by SDS-PAGE.

  • 克隆

    单克隆

  • 克隆编号

    MEM-G/1

  • 同种型

    IgG1

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7759于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P (1)
1/60 - 1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
WB (1)
Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 38.2 kDa.
说明
IHC-P
1/60 - 1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
WB
Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 38.2 kDa.

靶标

  • 功能

    Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells.

  • 组织特异性

    Expressed in trophoblasts.

  • 序列相似性

    Belongs to the MHC class I family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

  • 细胞定位

    Membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • B2 microglobulin antibody
      • DADB-15K14.8 antibody
      • HLA 6.0 antibody
      • HLA class I histocompatibility antigen alpha chain G antibody
      • HLA class I histocompatibility antigen, alpha chain G antibody
      • HLA class I molecule antibody
      • HLA G antibody
      • HLA G antigen antibody
      • HLA G histocompatibility antigen class I G antibody
      • HLA G3 antibody
      • HLA-G antibody
      • HLA-G histocompatibility antigen, class I antibody
      • HLA60 antibody
      • HLAG antibody
      • HLAG_HUMAN antibody
      • Major histocompatibility complex class I G antibody
      • MHC class I antigen antibody
      • MHC class I antigen G antibody
      • MHC G antibody
      • T-cell A locus antibody
      • TCA antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA G antibody [MEM-G/1] (ab7759)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA G antibody [MEM-G/1] (ab7759)

      Immunohistochemistry staining with ab7759 of first trimester placenta (paraffin-embedded sections).

    • Western blot - Anti-HLA G antibody [MEM-G/1] (ab7759)
      Western blot - Anti-HLA G antibody [MEM-G/1] (ab7759)
      All lanes : Anti-HLA G antibody [MEM-G/1] (ab7759) at 10 µg/ml

      Lane 1 : Human placenta tissue lysate - total protein (ab29745)
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 3 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Predicted band size: 38.2 kDa
      Observed band size: 42 kDa why is the actual band size different from the predicted?
      Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA G antibody [MEM-G/1] (ab7759)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA G antibody [MEM-G/1] (ab7759)
      The antibody ab7759 stains infiltrating macrophages in pulmonary diseases. In the top left corner see detail of macropage.
    • Western blot - Anti-HLA G antibody [MEM-G/1] (ab7759)
      Western blot - Anti-HLA G antibody [MEM-G/1] (ab7759)
      All lanes : Anti-HLA G antibody [MEM-G/1] (ab7759) at 2 µg/ml

      Lane 1 : JEG-3 cell line under reducing conditions.
      Lane 2 : LNCap cell line under reducing conditions.
      Lane 3 : JEG-3 cell line under non-reducing conditions.
      Lane 4 : LNCap cell line under non-reducing conditions.

      Secondary
      All lanes : IRDye800-conjugated anti-mouse secondary antibody

      Predicted band size: 38.2 kDa
      Observed band size: 40 kDa why is the actual band size different from the predicted?



      Western blotting analysis of human HLA-G using ab7759 on lysates of JEG-3 cell line and LNCaP cell line (negative control) under reducing and non-reducing conditions.

      Nitrocellulose membrane was probed with 2 µg/ml of ab7759 followed by IRDye800-conjugated anti-mouse secondary antibody. 

    文献 (21)

    发表研究结果有使用 ab7759？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab7759 被引用在 21 文献中.

    • Shahbazi MN  et al. Developmental potential of aneuploid human embryos cultured beyond implantation. Nat Commun 11:3987 (2020). PubMed: 32778678
    • Kudo Y  et al. Indoleamine 2,3-dioxygenase and trophoblast invasion in caesarean scar pregnancy: Implications for the aetiopathogenesis of placenta accreta spectrum. J Reprod Immunol 138:103099 (2020). PubMed: 32050141
    • Swets M  et al. HLA-G protein expression in colorectal cancer evaluated by immunohistochemistry and western blot analysis: Its expression characteristics remain enigmatic. Clin Immunol 194:80-86 (2018). PubMed: 30006120
    • Guo W  et al. Decreased Human Leukocyte Antigen-G Expression by miR-133a Contributes to Impairment of Proinvasion and Proangiogenesis Functions of Decidual NK Cells. Front Immunol 8:741 (2017). WB . PubMed: 28702027
    • Westwood M  et al. Vitamin D attenuates sphingosine-1-phosphate (S1P)-mediated inhibition of extravillous trophoblast migration. Placenta 60:1-8 (2017). PubMed: 29208234
    View all Publications for this product

    客户评价及客户问答

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    提交评价 提交问题

    1-10 of 13 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HLA G antibody [MEM-G/1]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Placenta)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9,0
    Permeabilization
    Yes - Wash Buffer from Dako with Tween
    Specification
    Placenta
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Rudolf Jung

    Verified customer

    提交于 Feb 18 2016

    Western blot abreview for Anti-HLA G antibody [MEM-G/1]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (JEG-3)
    Loading amount
    30 µg
    Specification
    JEG-3
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jun 01 2010

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-HLA G antibody [MEM-G/1]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Human Tissue sections (Placenta, extravillous cytotrophoblasts)
    Specification
    Placenta, extravillous cytotrophoblasts
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Richard Bruggeman

    Verified customer

    提交于 Jun 30 2005

    Question

    Could you tell me if the immunogen used to produce this antibody was made in bacteria or eukaryotic cells? Thank you,

    Read More
    Answer

    Thank you for your enquiry. I can confirm that the recombinant protein was produced in bacteria. If there is anything else that I can help you with, please do not hesitate to contact me.

    Read More

    Question

    Please recommend a starting dilution for IHC-p. Thanks.

    Read More
    Answer

    Thank you for your enquiry. I would recommend a starting dilution of 1/100, a concentration of 10 ug/mL, for this antibody in IHC-P. It is quite common to use a concentration between 1-20 ug/mL for IHC experiments. For this particular antibody we have received a review from a customer in which the antibody was used at a dilution of 1/200 on formalin fixed sections of human placenta extravillous cytotrophoblasts (not paraffin embedded). My recommendation of 1/100 is a starting dilution and you may have to optimize this dilution for your tissue. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    Please let me know the price tag and how to place an order if i decide to do so? Specification sheet is requested.

    Read More
    Answer

    Thank you for your enquiry regarding ab7759. The product datasheet for this antibody can be viewed by logging onto our website at www.Abcam.com and entering ab7759 in the search field. All relevant information such as price, technical data and availability can be viewed on this datasheet. To place an order, please get in touch with one of our experienced distribution partner, Gene Research Lab Co Ltd 3Fl., No.32, Sec.2, Jungyang S.Rd Beitou Dist Taipei Taiwan 112 ROC Email:sue@gene-rl.com Phone:+886-2-28925609 Fax:+882-2-28925602 Web:www.gene-rl.com Please contact us again if you require further assistance.

    Read More

    Question

    I want to purchase an human HLA-G1 monoclonal Antibody (anti what ever). I found one product (ab7759) which may be what I am after however I need clarrification. Does the MEM-G/1 refer to the isotype of the HLA-G

    Read More
    Answer

    Thank you for your enquiry and your interest in our products. We would like to confirm that ab7759 is a monoclonal antibody which recognizes human HLA-G1 and MEM-G/1 is the clone number. Please take a look at the on-line datasheet of this product, you will find useful information.

    Read More

    Question

    Where does HLA-G come out on a Western Blot? (MW-dKa)

    Read More
    Answer

    HLA-G1 usually moves as 39 kDa band. Other isoforms (G2, G3, G4, G5,) are also detected if present in your sample.

    Read More

    Question

    What would you suggest for a starting dilution for Immunohistochemical analysis, with both FFPE and also just fomalin fixed sections.

    Read More
    Answer

    Try this antibody at 1:100 and adjust accordingly from the results obtained. Recommended epitope retrieval is performed by microwave heating in citrate buffer, pH 6.0, 15 min.

    Read More

    Question

    I noticed that the antibody does not react with native HLA-G. Therefore, is a special procedure required for immunohistochemistry, specifically formalin fixed sections?

    Read More
    Answer

    This antibody reacts with denaturated heavy chain of HLA-G. Routine immunohistochemical protocol for staining of formalin-fixed, paraffin embedded sections is applicable. Recommended epitope retrieval is performed by microwave heating in citrate buffer, pH 6, 15 min.

    Read More

    1-10 of 13 Abreviews or Q&A

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