重组Anti-HLA-C抗体[EPR26121-73] - BSA and Azide free (ab307362)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR26121-73] to HLA-C - BSA and Azide free
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-HLA-C抗体[EPR26121-73] - BSA and Azide free
参阅全部 HLA-C 一抗 -
描述
兔单克隆抗体[EPR26121-73] to HLA-C - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, IPmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- WB: Jurkat transfected with scrambled siRNA control, Jurkat transfected with siRNA specifically targeting HLA-C whole cell lysate, A375, HeLa, Human lymphoma, HL-60, Human colon, and HEK-293T IHC-P: Human tonsil, Human NKT lymphoma and Human colon tissues. IP: Jurkat cell.
-
常规说明
ab307362 is the carrier-free version of ab307361
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.20
Constituent: 100% PBS -
无载体
是 -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR26121-73 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab307362于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 41 kDa.
|
|
IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
IP |
Use at an assay dependent concentration.
|
说明 |
---|
WB
Use at an assay dependent concentration. Predicted molecular weight: 41 kDa. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Use at an assay dependent concentration. |
靶标
- Information by UniProt
-
数据库链接
- Entrez Gene: 3107 Human
- Omim: 142840 Human
- SwissProt: P04222 Human
- SwissProt: P10321 Human
- SwissProt: Q07000 Human
- Unigene: 656020 Human
- Unigene: 743218 Human
- Unigene: 77961 Human
-
别名
- 1C07_HUMAN antibody
- Cw-7 alpha chain antibody
- D6S204 antibody
see all
图片
-
All lanes : Anti-HLA-C antibody [EPR26121-73] (ab307361) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia T lymphocyte) transfected with scrambled siRNA control whole cell lysate 20 µg
Lane 2 : Jurkat transfected with siRNA specifically targeting HLA-C whole cell lysate 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
Exposure time: 26 secondsThis data was developed using ab307361, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 26 seconds
-
All lanes : Anti-HLA-C antibody [EPR26121-73] (ab307361) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia T lymphocyte) whole cell lysate 80 µg
Lane 2 : A375 (human malignant melanoma epithelial cell) whole cell lysate 80 µg
Lane 3 : HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate 80 µg
Lane 4 : Human lymphoma tissue lysate 80 µg
Lane 5 : HL-60 (human acute promyelocytic leukemia promyeloblast) whole cell lysate 80 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
Exposure time: 15 secondsThis data was developed using ab307361, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 15 seconds
-
Anti-HLA-C antibody [EPR26121-73] (ab307361) at 1/1000 dilution + Human colon tissue lysate 40 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
Exposure time: 7 secondsThis data was developed using ab307361, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 7.25 seconds
-
All lanes : Anti-HLA-C antibody [EPR26121-73] (ab307361) at 1/1000 dilution
Lane 1 : HEK-293T cells transfected with an empty vector containing a his tag whole cell lysate 20 µg
Lane 2 : HEK-293T cells transfected with a human HLA-C expression vector containing a his tag whole cell lysate 20 µg
Lane 3 : HEK-293T cells transfected with a human HLA-A expression vector containing a his tag whole cell lysate 20 µg
Lane 4 : HEK-293T cells transfected with a human HLA-B expression vector containing a his tag whole cell lysate 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
Exposure time: 3 secondsThis data was developed using ab307361, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This antibody does not cross-react with human HLA-A and HLA-B.
Exposure time: 3 seconds
-
This data was developed using ab307361, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling HLA-C with ab307361 at 1/4000 (0.126 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human tonsil (PMID: 17008885). The section was incubated with ab307361 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 10 mins
-
This data was developed using ab307361, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human NKT lymphoma tissue labeling HLA-C with ab307361 at 1/4000 (0.126 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on NKT lymphoma. The section was incubated with ab307361 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 10 mins
-
This data was developed using ab307361, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling HLA-C with ab307361 at 1/4000 (0.126 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human colon (PMID: 17008885). The section was incubated with ab307361 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 10 mins
-
This data was developed using ab307361, the same antibody clone in a different buffer formulation.
HLA-C was immunoprecipitated from 0.35 mg Jurkat (human T cell leukemia T lymphocyte) whole cell lysate 5 ug with ab307361 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307361 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Jurkat (human T cell leukemia T lymphocyte) whole cell lysate 5 ug
Lane 2: abab307361 IP in Jurkat whole cell lysate
Lane 3:Rabbit monoclonal IgG (ab172730) instead of ab307361 in Jurkat whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
Datasheet download
Certificate of Compliance
文献 (0)
ab307362 尚未被引用在任何文献中。