Application
ELISA
Sample
Human Cell (In Cell ELISA CRC SW620 cell line)
Specification
In Cell ELISA CRC SW620 cell line
Type
Direct
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Diluent: plus on 4C
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit 800 Li-COR
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 800CW
Dilution
1/2000
Additional data
Additional Notes
I used 2 primary antibodies the anti-MHC classI and the Anti-Carcino Embryonic Antigen CEA antibody ab15987
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Rita Fior
Verified customer
提交于 Jul 14 2014