Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Human glioma Xenograft in mouse brain)
Specification
Human glioma Xenograft in mouse brain
Fixative
Paraformaldehyde
Permeabilization
Yes - 1% Triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
Other product details
Dilution
1/200
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking solution
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 647 goat-anti rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Dilution
1/1000
Additional data
Additional Notes
The image shows MHC class I antibody in red, Human Nuclear antigen in green and dapi in blue.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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提交于 Jul 14 2011