Anti-HJURP抗体(ab100800)
Key features and details
- Rabbit polyclonal to HJURP
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-HJURP抗体
参阅全部 HJURP 一抗 -
描述
兔多克隆抗体to HJURP -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human HJURP aa 650-750. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q8NCD3 -
常规说明
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified using an epitope specific to HJURP immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab100800于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.04 µg/ml. Predicted molecular weight: 84 kDa.
|
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| IP |
Use at 2-5 µg/mg of lysate.
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| IHC-P |
1/5000.
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| 说明 |
|---|
|
WB
Use a concentration of 0.04 µg/ml. Predicted molecular weight: 84 kDa. |
|
IP
Use at 2-5 µg/mg of lysate. |
|
IHC-P
1/5000. |
靶标
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相关性
Centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. Acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. Directly binds Holliday junctions. -
细胞定位
Nucleus; nucleolus. Centromere. Note: Localizes in centromeres during late telophase and early G1, when CENPA nucleosomes are assembled. Localizes to nucleolus during S phase, nucleolus site being often related to storage. -
数据库链接
- Entrez Gene: 55355 Human
- Omim: 612667 Human
- SwissProt: Q8NCD3 Human
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别名
- 14-3-3-associated AKT substrate antibody
- FAKTS antibody
- fetal liver expressing gene 1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HJURP antibody (ab100800)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling HJURP with ab100800 at 1/5000 (0.2µg/mg). Detection: DAB.
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Western blot - Anti-HJURP antibody (ab100800)All lanes : Anti-HJURP antibody (ab100800) at 0.04 µg/ml
Lane 1 : K-562 whole cell lysate
Lane 2 : U2OS whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : MCF-7 whole cell lysate
Lysates/proteins at 2 µg per lane.
Predicted band size: 84 kDa -
Immunoprecipitation - Anti-HJURP antibody (ab100800)ab100800 at 1 µg/ml staining HJURP in HeLa cell lysate immunoprecipitated using ab100800 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Exposure time: 30 seconds
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab100800 被引用在 6 文献中.
- Lai W et al. HJURP promotes proliferation in prostate cancer cells through increasing CDKN1A degradation via the GSK3ß/JNK signaling pathway. Cell Death Dis 12:583 (2021). PubMed: 34099634
- Yuan JS et al. Holliday junction-recognition protein modulates apoptosis, cell cycle arrest and reactive oxygen species stress in human renal cell carcinoma. Oncol Rep 44:1246-1254 (2020). PubMed: 32582972
- Pan D et al. Mechanism of centromere recruitment of the CENP-A chaperone HJURP and its implications for centromere licensing. Nat Commun 10:4046 (2019). PubMed: 31492860
- Liu C & Mao Y Diaphanous formin mDia2 regulates CENP-A levels at centromeres. J Cell Biol 213:415-24 (2016). PubMed: 27185834
- Montes de Oca R et al. The histone chaperone HJURP is a new independent prognostic marker for luminal A breast carcinoma. Mol Oncol 9:657-74 (2015). PubMed: 25497280
- Bravaccini S et al. New biomarkers to predict the evolution of in situ breast cancers. Biomed Res Int 2014:159765 (2014). IHC ; Human . PubMed: 25243117