Histone H4 Total Quantification试剂盒(Colorimetric) (ab156909)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 3 hr 30 min
- Sample type: Cell culture extracts, Nuclear Extracts, Tissue Extracts
- Sensitivity: 0.5 ng/well
概述
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产品名称
Histone H4 Total Quantification试剂盒(Colorimetric)
参阅全部 Histone H4 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture extracts, Tissue Extracts, Nuclear Extracts -
检测类型
Quantitative -
灵敏度
>= 0.5 ng/well -
范围
50 ng - 1000 ng -
检测时间
3h 30m -
种属反应性
与反应: Mouse, Rat, Human -
产品概述
Abcam's Histone H4 Total Quantification Kit (Colorimetric) is a complete set of essential components which enables an experimenter to colorimetrically quantify levels of histone H4 proteins independent of their modified states. It can also be used for normalizing the modified histone H4 content of samples when run in parallel with Abcam's histone modification quantification kit series.
The kit is suitable for specifically measuring total histone H4 using human, mouse, and rat samples, including fresh and frozen tissues and cultured adherent and suspension cells.
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平台
Microplate reader
性能
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存放说明
Please refer to protocols. -
组件 48 tests 96 tests 1000X Capture Antibody 1 x 5µl 1 x 10µl 10X Wash Buffer 1 x 14ml 1 x 28ml 2000X Signal Reporter 1 x 6µl 1 x 12µl 8-Well Assay Strips (with Frame) 1 x 6 units 1 x 12 units Color Developer 1 x 5ml 1 x 10ml Enhancer Solution 1 x 120µl 1 x 240µl Histone Assay Buffer 1 x 4ml 1 x 8ml Standard Control, 100 µg/mL 1 x 10µl 1 x 20µl Stop Solution 1 x 5ml 1 x 10ml -
研究领域
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相关性
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. -
细胞定位
Nuclear -
别名
- H4/m
- H4FA
- H4FM
see all -
数据库链接
- Entrez Gene: 8294 Human
- Entrez Gene: 319155 Mouse
- Entrez Gene: 64627 Rat
- Omim: 602833 Human
- SwissProt: P62805 Human
- SwissProt: P62806 Mouse
- SwissProt: P62804 Rat
相关产品
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Related Products
- Histone Extraction Kit (ab113476)
- Histone H3 Total Quantification Kit (Colorimetric) (ab115091)
- Histone H4 (acetyl K5) Quantification Kit (Fluorometric) (ab115117)
- Histone H4 (acetyl K8) Quantification Kit (Colorimetric) (ab115118)
- Histone H4 (acetyl K8) Quantification Kit (Fluorometric) (ab115119)
- Histone H4 (acetyl K16) Quantification Kit (Colorimetric) (ab115120)
- Histone H4 (acetyl K16) Quantification Kit (Fluorometric) (ab115121)
- Histone H4 (acetyl K12) Quantification Kit (Colorimetric) (ab115122)
- Histone H4 (acetyl K12) Quantification Kit (Fluorometric) (ab115123)
图片
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Total H4 per mg of extracted histones from HeLa control cells (C) or cells treated with 10 micromolar sodium butyrate (But) (extracted using ab113476; 50-500 ng per well) (duplicates +/- SD).
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Total H4 per mg of extracted histones from murine tissue preparations (extracted using ab113476; 50-500 ng per well) (duplicates +/- SD).
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Standard curve with background signal subtracted (duplicates; +/- SD).
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Histone nuclear extracts were prepared from MDA-231 cells using the Histone Extraction Kit (ab113476) and the ODs generated from Histone H4 are measured.
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Figure 1. Illustrated standard curve generated with H4 standard.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab156909 被引用在 1 文献中.
- Sanders NL et al. Neutrophil Extracellular Traps as an Exacerbating Factor in Bacterial Pneumonia. Infect Immun 90:e0049121 (2022). PubMed: 35130455