Application
Western blot
Sample
Mouse Cell lysate - whole cell (Neuro2A- neuroblastoma)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
40 µg
Specification
Neuro2A- neuroblastoma
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST containing 0.2% BSA
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Anti Rabbit IgG-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Perkin Elmer ECL reagent
Negative control
nil
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 11 kDa
Positive control
nil
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jun 07 2013