Anti-Histone H4 (mono methyl R3)抗体(ab17339)
Key features and details
- Rabbit polyclonal to Histone H4 (mono methyl R3)
- Suitable for: WB, ICC/IF, PepArr
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Histone H4 (mono methyl R3)抗体
参阅全部 Histone H4 一抗 -
描述
兔多克隆抗体to Histone H4 (mono methyl R3) -
宿主
Rabbit -
特异性
ab17339 detects a 14 kDa band in single lane Western Blot. Peptide inhibition in Western Blot hasn't been processed. Modification specificity is determined by Peptide Array. ab17339 binds strongly to the Histone H4 mono methyl R3 peptide. From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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经测试应用
适用于: WB, ICC/IF, PepArrmore details -
种属反应性
与反应: Mouse, Rat, Cow, Human
预测可用于: Pig -
免疫原
Synthetic peptide within Human Histone H4 aa 1-100 (N terminal) (mono methyl R3) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
(Peptide available asab17770) -
阳性对照
- WB: Calf thymus histone, HL60 whole cell, HeLa nuclear - triton prep, NIH3T3 nuclear- triton prep, PC12 nuclear - triton prep, HL60 whole cell lysate. ICC/IF: HepG2 cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab17339于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 15 kDa).
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ICC/IF |
Use a concentration of 1 µg/ml.
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PepArr |
Use a concentration of 0.2 - 0.02 µg/ml.
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 15 kDa). |
ICC/IF
Use a concentration of 1 µg/ml. |
PepArr
Use a concentration of 0.2 - 0.02 µg/ml. |
靶标
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功能
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. -
序列相似性
Belongs to the histone H4 family. -
翻译后修饰
Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
Sumoylated, which is associated with transcriptional repression. -
细胞定位
Nucleus. Chromosome. - Information by UniProt
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数据库链接
- Entrez Gene: 121504 Human
- Entrez Gene: 554313 Human
- Entrez Gene: 8294 Human
- Entrez Gene: 8359 Human
- Entrez Gene: 8360 Human
- Entrez Gene: 8361 Human
- Entrez Gene: 8362 Human
- Entrez Gene: 8363 Human
see all -
别名
- dJ160A22.1 antibody
- dJ160A22.2 antibody
- dJ221C16.1 antibody
see all
图片
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ab17339 staining Histone H4 (mono methyl R3) in HepG2 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab17339 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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All lanes : Anti-Histone H4 (mono methyl R3) antibody (ab17339) at 1 µg/ml
Lane 1 : Calf thymus histone at 0.1 µg with BSA
Lane 2 : HL60 whole cell at 10 µg with BSA
Lane 3 : HeLa Nuclear - Triton prep at 10 µg with BSA
Lane 4 : NIH3T3 Nuclear - Triton prep at 10 µg with BSA
Lane 5 : PC-12 Nuclear - Triton prep at 10 µg with BSA
Blocking peptides at 2 % per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
Predicted band size: 15 kDa
Exposure time: 2 minutes -
Anti-Histone H4 (mono methyl R3) antibody (ab17339) at 1 µg/ml + HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted? -
All batches of ab17339 are tested in Peptide Array against peptides to different Histone H4 modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H4 - mono methyl R3 peptide (ab17770), indicating that this antibody specifically recognises the Histone H4 - mono methyl R3 modification.
ab17770 - Histone H4 - mono methyl R3
ab15821 - Histone H4 - unmodified
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab17339 被引用在 13 文献中.
- Liu J et al. Maternal Diabetes-Induced Suppression of Oxytocin Receptor Contributes to Social Deficits in Offspring. Front Neurosci 15:634781 (2021). PubMed: 33633538
- Ma Z et al. Symmetrical dimethylation of H4R3: A bridge linking DNA damage and repair upon oxidative stress. Redox Biol 37:101653 (2020). PubMed: 32739156
- Xiang D et al. Betulinic Acid Inhibits Endometriosis Through Suppression of Estrogen Receptor ß Signaling Pathway. Front Endocrinol (Lausanne) 11:604648 (2020). PubMed: 33362719
- Liu M et al. Characterization of protein arginine methyltransferase of TgPRMT5 in Toxoplasma gondii. Parasit Vectors 12:221 (2019). PubMed: 31068219
- Demetriadou C et al. NAA40 contributes to colorectal cancer growth by controlling PRMT5 expression. Cell Death Dis 10:236 (2019). PubMed: 30858358