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Epigenetics and Nuclear Signaling Histones H4 Acetylated

Anti-Histone H4 (acetyl K91)抗体- ChIP Grade (ab4627)

  • Datasheet
Reviews (12) Submit a question References (12)

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ChIP - Anti-Histone H4 (acetyl K91) antibody - ChIP Grade (ab4627)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K91) antibody - ChIP Grade (ab4627)
  • Western blot - Anti-Histone H4 (acetyl K91) antibody - ChIP Grade (ab4627)

Key features and details

  • Rabbit polyclonal to Histone H4 (acetyl K91) - ChIP Grade
  • Suitable for: IHC-P, WB, ChIP
  • Reacts with: Cow, Human, Saccharomyces cerevisiae
  • Isotype: IgG

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概述

  • 产品名称

    Anti-Histone H4 (acetyl K91)抗体- ChIP Grade
    参阅全部 Histone H4 一抗
  • 描述

    兔多克隆抗体to Histone H4 (acetyl K91) - ChIP Grade
  • 宿主

    Rabbit
  • 经测试应用

    适用于: IHC-P, WB, ChIPmore details
  • 种属反应性

    与反应: Cow, Human, Saccharomyces cerevisiae
    预测可用于: Mouse, Rat, Chicken, Pig, Xenopus laevis, Caenorhabditis elegans, Drosophila melanogaster, Duck
  • 免疫原

    Synthetic peptide corresponding to Human Histone H4 aa 50 to the C-terminus (acetyl K91) conjugated to keyhole limpet haemocyanin.
    Database link: P62805
    (Peptide available as ab10112)

  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • Histones
    • H4
    • Acetylated
    • Epigenetics and Nuclear Signaling
    • ChIP assays
    • ChIP antibodies

相关产品

  • ChIP Related Products

    • Rabbit Anti-Mouse IgG H&L (ab46540)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Histone H4 protein (ab198115)

应用

Our Abpromise guarantee covers the use of ab4627 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 12 kDa).
ChIP Use at an assay dependent concentration.

靶标

  • 功能

    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • 序列相似性

    Belongs to the histone H4 family.
  • 翻译后修饰

    Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
    Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
    Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
    Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
    Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
    Sumoylated, which is associated with transcriptional repression.
  • 细胞定位

    Nucleus. Chromosome.
  • Target information above from: UniProt accession P62805 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 318846 Drosophila melanogaster
    • Entrez Gene: 3771854 Drosophila melanogaster
    • Entrez Gene: 3771893 Drosophila melanogaster
    • Entrez Gene: 3771908 Drosophila melanogaster
    • Entrez Gene: 3771935 Drosophila melanogaster
    • Entrez Gene: 3771938 Drosophila melanogaster
    • Entrez Gene: 3771941 Drosophila melanogaster
    • Entrez Gene: 3771947 Drosophila melanogaster
    • Entrez Gene: 3772096 Drosophila melanogaster
    • Entrez Gene: 3772113 Drosophila melanogaster
    • Entrez Gene: 3772129 Drosophila melanogaster
    • Entrez Gene: 3772172 Drosophila melanogaster
    • Entrez Gene: 3772211 Drosophila melanogaster
    • Entrez Gene: 3772254 Drosophila melanogaster
    • Entrez Gene: 3772314 Drosophila melanogaster
    • Entrez Gene: 3772317 Drosophila melanogaster
    • Entrez Gene: 3772319 Drosophila melanogaster
    • Entrez Gene: 3772325 Drosophila melanogaster
    • Entrez Gene: 3772509 Drosophila melanogaster
    • Entrez Gene: 3772519 Drosophila melanogaster
    • Entrez Gene: 3772666 Drosophila melanogaster
    • Entrez Gene: 3772708 Drosophila melanogaster
    • Entrez Gene: 41773 Drosophila melanogaster
    • Entrez Gene: 121504 Human
    • Entrez Gene: 554313 Human
    • Entrez Gene: 8294 Human
    • Entrez Gene: 8359 Human
    • Entrez Gene: 8360 Human
    • Entrez Gene: 8361 Human
    • Entrez Gene: 8362 Human
    • Entrez Gene: 8363 Human
    • Entrez Gene: 8364 Human
    • Entrez Gene: 8365 Human
    • Entrez Gene: 8366 Human
    • Entrez Gene: 8367 Human
    • Entrez Gene: 8368 Human
    • Entrez Gene: 8370 Human
    • Entrez Gene: 100041230 Mouse
    • Entrez Gene: 319155 Mouse
    • Entrez Gene: 319156 Mouse
    • Entrez Gene: 319157 Mouse
    • Entrez Gene: 319158 Mouse
    • Entrez Gene: 319159 Mouse
    • Entrez Gene: 319160 Mouse
    • Entrez Gene: 319161 Mouse
    • Entrez Gene: 320332 Mouse
    • Entrez Gene: 326619 Mouse
    • Entrez Gene: 326620 Mouse
    • Entrez Gene: 674678 Mouse
    • Entrez Gene: 69386 Mouse
    • Entrez Gene: 97122 Mouse
    • Entrez Gene: 64627 Rat
    • Entrez Gene: 447787 Xenopus laevis
    • Omim: 142750 Human
    • Omim: 602822 Human
    • Omim: 602823 Human
    • Omim: 602824 Human
    • Omim: 602825 Human
    • Omim: 602826 Human
    • Omim: 602827 Human
    • Omim: 602828 Human
    • Omim: 602829 Human
    • Omim: 602830 Human
    • Omim: 602831 Human
    • Omim: 602833 Human
    • SwissProt: P84040 Drosophila melanogaster
    • SwissProt: P62805 Human
    • SwissProt: P62806 Mouse
    • SwissProt: P62804 Rat
    • SwissProt: P02309 Saccharomyces cerevisiae
    • SwissProt: P62799 Xenopus laevis
    • Unigene: 21500 Drosophila melanogaster
    • Unigene: 29514 Drosophila melanogaster
    • Unigene: 29527 Drosophila melanogaster
    • Unigene: 30219 Drosophila melanogaster
    • Unigene: 30220 Drosophila melanogaster
    • Unigene: 30221 Drosophila melanogaster
    • Unigene: 30223 Drosophila melanogaster
    • Unigene: 30868 Drosophila melanogaster
    • Unigene: 30869 Drosophila melanogaster
    • Unigene: 30871 Drosophila melanogaster
    • Unigene: 30872 Drosophila melanogaster
    • Unigene: 30873 Drosophila melanogaster
    • Unigene: 30876 Drosophila melanogaster
    • Unigene: 33873 Drosophila melanogaster
    • Unigene: 5747 Drosophila melanogaster
    • Unigene: 143080 Human
    • Unigene: 247816 Human
    • Unigene: 248172 Human
    • Unigene: 248178 Human
    • Unigene: 248179 Human
    • Unigene: 278483 Human
    • Unigene: 46423 Human
    • Unigene: 528055 Human
    • Unigene: 533295 Human
    • Unigene: 55468 Human
    • Unigene: 591790 Human
    • Unigene: 655235 Human
    • Unigene: 662174 Human
    • Unigene: 706635 Human
    • Unigene: 718935 Human
    • Unigene: 144300 Mouse
    • Unigene: 14775 Mouse
    • Unigene: 158272 Mouse
    • Unigene: 227295 Mouse
    • Unigene: 228709 Mouse
    • Unigene: 246720 Mouse
    • Unigene: 255646 Mouse
    • Unigene: 260530 Mouse
    • Unigene: 261642 Mouse
    • Unigene: 261662 Mouse
    • Unigene: 261664 Mouse
    • Unigene: 377875 Mouse
    • Unigene: 442307 Mouse
    • Unigene: 85592 Xenopus laevis
    see all
  • 别名

    • dJ160A22.1 antibody
    • dJ160A22.2 antibody
    • dJ221C16.1 antibody
    • dJ221C16.9 antibody
    • FO108 antibody
    • H4 antibody
    • H4.k antibody
    • H4/a antibody
    • H4/b antibody
    • H4/c antibody
    • H4/d antibody
    • H4/e antibody
    • H4/g antibody
    • H4/h antibody
    • H4/I antibody
    • H4/j antibody
    • H4/k antibody
    • H4/m antibody
    • H4/n antibody
    • H4/p antibody
    • H4_HUMAN antibody
    • H4F2 antibody
    • H4F2iii antibody
    • H4F2iv antibody
    • H4FA antibody
    • H4FB antibody
    • H4FC antibody
    • H4FD antibody
    • H4FE antibody
    • H4FG antibody
    • H4FH antibody
    • H4FI antibody
    • H4FJ antibody
    • H4FK antibody
    • H4FM antibody
    • H4FN antibody
    • H4M antibody
    • HIST1H4A antibody
    • HIST1H4B antibody
    • HIST1H4C antibody
    • HIST1H4D antibody
    • HIST1H4E antibody
    • HIST1H4F antibody
    • HIST1H4H antibody
    • HIST1H4I antibody
    • HIST1H4J antibody
    • HIST1H4K antibody
    • HIST1H4L antibody
    • HIST2H4 antibody
    • HIST2H4A antibody
    • Hist4h4 antibody
    • Histone 1 H4a antibody
    • Histone 1 H4b antibody
    • Histone 1 H4c antibody
    • Histone 1 H4d antibody
    • Histone 1 H4e antibody
    • Histone 1 H4f antibody
    • Histone 1 H4h antibody
    • Histone 1 H4i antibody
    • Histone 1 H4j antibody
    • Histone 1 H4k antibody
    • Histone 1 H4l antibody
    • Histone 2 H4a antibody
    • histone 4 H4 antibody
    • Histone H4 antibody
    • MGC24116 antibody
    see all

图片

  • ChIP - Anti-Histone H4 (acetyl K91) antibody - ChIP Grade (ab4627)
    ChIP - Anti-Histone H4 (acetyl K91) antibody - ChIP Grade (ab4627)

    Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The  ChIP was performed with 25 µg of chromatin, 2 µg of  ab4627 (blue), and 20 µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K91) antibody - ChIP Grade (ab4627)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K91) antibody - ChIP Grade (ab4627)

    IHC image of ab4627 staining Histone H2A.X in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4627, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Western blot - Anti-Histone H4 (acetyl K91) antibody - ChIP Grade (ab4627)
    Western blot - Anti-Histone H4 (acetyl K91) antibody - ChIP Grade (ab4627)
    Lanes 1 & 3 : Anti-Histone H4 (acetyl K91) antibody - ChIP Grade (ab4627) at 1/500 dilution
    Lanes 2 & 4 : Anti-Histone H4 (acetyl K91) antibody - ChIP Grade (ab4627) at 1/1000 dilution

    Lanes 1-2 : Calf thymus histone prep.
    Lanes 3-4 : Calf thymus histone prep. with Human Histone H4 (acetyl K91) peptide (ab10112) at 2 µg

    Lysates/proteins at 1 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution

    Predicted band size: 12 kDa



    Rabbit polyclonal to Histone H4 acetyl K91 (ab4627). All lanes contain 1ug of calf thymus Histone prep.

    Lane 1: ab4627 at 1/500
    Lane 2: ab4627 at 1/1000
    Lane 3: ab4627 at 1/500 + 2ug acetyl K91 blocking peptide (ab10112)
    Lane 4: ab4627 at 1/1000 + 2ug acetyl K91 blocking peptide (ab10112)

    Secondary antibody: Goat anti-rabbit (HRP) - ab6721 at 1/2000

    Predicted Molecular weight11KDa

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

    • Datasheet
  • 文献 (12)

    发表研究结果有使用 ab4627?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab4627 被引用在 12 文献中.

    • Xiao Q  et al. Multiple Site-Specific One-Pot Synthesis of Two Proteins by the Bio-Orthogonal Flexizyme System. Front Bioeng Biotechnol 8:37 (2020). PubMed: 32117920
    • Getun IV  et al. Functional Roles of Acetylated Histone Marks at Mouse Meiotic Recombination Hot Spots. Mol Cell Biol 37:N/A (2017). PubMed: 27821479
    • Kieffer-Kwon KR  et al. Myc Regulates Chromatin Decompaction and Nuclear Architecture during B Cell Activation. Mol Cell 67:566-578.e10 (2017). PubMed: 28803781
    • Yang X  et al. Histone acetyltransferase 1 promotes homologous recombination in DNA repair by facilitating histone turnover. J Biol Chem 288:18271-82 (2013). Human . PubMed: 23653357
    • Singh P  et al. Allele-specific H3K79 Di- versus trimethylation distinguishes opposite parental alleles at imprinted regions. Mol Cell Biol 30:2693-707 (2010). PubMed: 20351169
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 12 Abreviews or Q&A

    Immunocytochemistry abreview for Anti-Histone H4 (acetyl K91) antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 23°C
    Sample
    Mouse Cultured Cells (C2C12 muscle fibroblast)
    Specification
    C2C12 muscle fibroblast
    Permeabilization
    Yes - 0.1% Triton X-100
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Feb 25 2014

    Western blot abreview for Anti-Histone H4 (acetyl K91) antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (15%)
    Sample
    Caenorhabditis elegans Tissue lysate - whole (C. elegans larvae)
    Specification
    C. elegans larvae
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    提交于 Feb 21 2014

    Western blot abreview for Anti-Histone H4 (acetyl K91) antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    15 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Fruit fly (Drosophila melanogaster) Tissue lysate - whole (Fruit fly embryo)
    Specification
    Fruit fly embryo
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    提交于 Feb 21 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H4 (acetyl K91) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 37 minute(s) · Concentration: 3% · Temperature: 37°C
    Sample
    Mouse Cell (NIH3T3)
    Specification
    NIH3T3
    Permeabilization
    Yes - 0.25% ~0.5% Triton
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Dec 20 2013

    Immunoprecipitation abreview for Anti-Histone H4 (acetylated) (acetyl K91) antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Saccharomyces cerevisiae Cell lysate - nuclear (BY4147 background)
    Total protein in input
    1e+006 cells
    Specification
    BY4147 background
    Treatment
    0.75% MMS (DNA damaging agent)
    Immuno-precipitation step
    Protein G
    Read More

    Abcam user community

    Verified customer

    提交于 Aug 09 2011

    Western blot abreview for Anti-Histone H4 (acetylated) (acetyl K91) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Saccharomyces cerevisiae Cell lysate - whole cell (S288c)
    Loading amount
    25 µg
    Specification
    S288c
    Gel Running Conditions
    Reduced Denaturing (15% gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Read More

    Mr. Harsh Ja

    Verified customer

    提交于 Aug 02 2011

    ChIP abreview for Anti-Histone H4 (acetylated) (acetyl K91) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Saccharomyces cerevisiae Cell lysate - nuclear (S288c)
    Specification
    S288c
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 15 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 1% Fromaldehyde
    Detection step
    Real-time PCR
    Read More

    Mr. Harsh Ja

    Verified customer

    提交于 Aug 02 2011

    ChIP abreview for Anti-Histone H4 (acetylated) (acetyl K91) antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Saccharomyces cerevisiae Cell lysate - nuclear (W303 background)
    Specification
    W303 background
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 20 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 1% Formaldehyde
    Detection step
    Semiquantitative PCR
    Read More

    Abcam user community

    Verified customer

    提交于 May 31 2011

    ChIP on chip abreview for Anti-Histone H4 (acetylated) (acetyl K91) antibody - ChIP Grade

    Average
    Abreviews
    Abreviews
    Application
    ChIP on chip
    Sample
    Saccharomyces cerevisiae Cell lysate - nuclear (W303 background)
    Specification
    W303 background
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 20 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 1% Formaldehyde
    Detection step
    Microarray
    Negative control
    No antibody control
    Read More

    Abcam user community

    Verified customer

    提交于 May 31 2011

    Western blot abreview for Anti-Histone H4 (acetylated) (acetyl K91) antibody - ChIP Grade

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    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Saccharomyces cerevisiae Cell lysate - whole cell (Yeast BY4741 background)
    Loading amount
    10 µg
    Specification
    Yeast BY4741 background
    Gel Running Conditions
    Reduced Denaturing (15% gel)
    Blocking step
    Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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    提交于 May 26 2011

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