Application
Flow Cytometry
Sample
Mouse Cell (T lymphocytes)
Permeabilization
Yes - eBioscience FoxP3/TF staining kit
Gating Strategy
Gated on CD3+/Thy+ cells
Specification
T lymphocytes
Preparation
Cell harvesting/tissue preparation method: Extracted from spleen, by magnetic negative selection
Sample buffer: FACS buffer (PBS + 2% SVF + 0.5 mM EDTA + 0.01% azide)
Sample buffer: FACS buffer (PBS + 2% SVF + 0.5 mM EDTA + 0.01% azide)
Fixation
Formaldehyde
Other product details
Incubation time
15 minute(s) · Temperature: 4°C
Dilution
1/100
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Goatanti-rabbit IgG-AF488 (Molecular Probes # A110
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
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提交于 Jun 20 2017