Western blot abreview for Anti-Histone H3 (tri methyl K4) antibody [mAbcam1012] - ChIP Grade

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Mammary cell line)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
1e+006 cells
Specification
Mammary cell line
Blocking step
Milk as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: Goat anti Mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 17 kDa

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提交于 Feb 28 2017

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