Application
Immunocytochemistry
Sample
Human Cultured Cells (primary fibroblasts)
Specification
primary fibroblasts
Fixative
Paraformaldehyde
Permeabilization
Yes - TritonX100 and saponin
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Other product details
Dilution
1/200
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit IgG-FITC (#F9887, Sigma)
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Fluorescein
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Fluorescein
Dilution
1/200
Additional data
Additional Notes
We used combination of following antibodies: Anti-H3K4me3 (rabbit) from Abcam + anti-HP1alpha (clone 15.19s2 Upstate #05-689, mouse) + anti-lamin A/C (346) (#sc-7293, Santa Cruz, mouse).
As a secondary antibodies we used: Anti-rabbit IgG-FITC (#F9887, Sigma) and Alexa Fluor 594 goat anti-mouse IgG (#A11005, Molecular Probes).
As a secondary antibodies we used: Anti-rabbit IgG-FITC (#F9887, Sigma) and Alexa Fluor 594 goat anti-mouse IgG (#A11005, Molecular Probes).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Eva Bartova
Verified customer
提交于 Nov 23 2007