Application
ChIP
Sample
Human Cell lysate - whole cell (esophagus)
Negative control
IgG
Specification
esophagus
Detection step
Real-time PCR
Type
Native ChIP (N-ChIP)
Positive control
polymerase ii
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: bsa
Concentration
2 µl/µg chromatin
Additional data
Additional Notes
I used the ab185913-high sensitivity chip kit.
Fold Enrichment calculation:FE can be calculated by simply using a ration of amplication effciency of the ChIP sample over that of Non-Immune IgG. Amplification efficiency of RNA polymerase ll can be used as a positive control. FE%=2^(IgG CT-SampleCT)*100%
The difference between the first and second bars is that the second bar is a sample with EZH2 silencing.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Aug 14 2017