Application
Western blot
Sample
Chinese hamster Cell lysate - whole cell (Chinese hamster ovary (CHO) cells)
Gel Running Conditions
Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel (MES buffer))
Loading amount
50000 cells
Specification
Chinese hamster ovary (CHO) cells
Blocking step
Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Li-Cor Odyssey Blocking Buffer (TBS)
Dilution
1/1000
Secondary antibody
Dilution
1/15000
Name
Non-Abcam antibody was used: IRDye 800CW Goat anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 800CW
Detection
Detection method
Near-Infrared Fluorescence Imaging (NIR)
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 18 kDa
Additional data
Additional Notes
Both Anti-Histone H3 (phospho S10) antibody and anti-beta actin (ab6276) antibodies were used for the western blot. The Anti-Histone H3 (phospho S10) antibody detected a specific band at 18 kDa (green band). The 42 kDa red band is the beta actin band (red band).
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提交于 Dec 31 2016