Application
Western blot
Sample
Human Cell lysate - nuclear (Hela)
Loading amount
25 µg
Specification
Hela
Gel Running Conditions
Reduced Denaturing (15%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Other product details
Dilution
1/3000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS+Tween
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 16 kDa
Additional data
Abcam response
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Abcam user community
Verified customer
提交于 Jun 20 2012