Application
Western blot
Sample
Rana sylvatica Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (15% Tris-glycine)
Loading amount
40 µg
Specification
Liver
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: RT°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/1000
Secondary antibody
Dilution
1/8000
Name
Non-Abcam antibody was used: Anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
7 minute(s) and 0 second(s)
Bands
Specific: 17 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Liam Hawkins
Verified customer
提交于 Jan 15 2016