概述

  • 产品名称
    Histone H3 (K4) Methyltransferase Activity Quantification Assay试剂盒
    参阅全部 Histone Methyltransferase 试剂盒
  • 样品类型
    Tissue Extracts, Cell Lysate
  • 检测类型
    Quantitative
  • 检测时间
    3h 00m
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Mammals
  • 产品概述

    Methylation of histone H3 at lysine 4 (K4) is thought to be a global epigenetic mark in euchromatin and a mediator of activated transcription. In mammalian cells, several SET-domain containing histone lysine methyltransferases such as MLL1 are responsible for adding one, two or three methyl groups at lysine 4 of histone H3.
    Histone H3 (K4) Methyltransferase Activity Quantification Assay Kit (ab113452) allows the user to measure the activity or inhibition of individual histone methyltransferases that specifically target histone H3 at lysine 4 (H3K4). The kit is ready-to-use and provides all the essential components needed to carry out a successful histone methyltransferase activity/inhibition quantification assay without the need for radioactivity or any special equipment.

  • 平台
    Microplate reader

性能

图片

  • Nuclear extracts were prepared from MCF-7 cells using the ab113474 and H3-K4 specific histone methyltrasferase activity (SET7/9) was measured.

实验方案

文献

ab113452 has not yet been referenced specifically in any publications.

客户评价及客户问答

1-3 of 3 Abreviews or Q&A

Answer

Thank you contacting us with these further questions. I have spoken with the lab about a usefull concentration for the purified HMT enzyme. Based on tests, we conclude that the useful concentration range can be from 10 ng to 200 ng/well (30 ul). I also wanted to let you know that SMYD3 (ab80377), as di-and tri-methyltransferase against H3-K4, can be definitely used with this kit. If you have any other questions or there is anything else we can do to to help you reach your research goals, please contact us.

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Answer

Thank you for contacting us. I have had a chance to speak with the lab regarding your question. They have told me that the purified HMT enzyme (H3-K4 specific) can be used in this assay kit. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your enquiry regarding ab113452.
1. The biotinylated substrate is unmethylated histone peptide and used for H3-K9 specific histone methyltransferase reaction.
2. For generating a standard curve, first, dilute HK5 HMT Standard with HK2 Assay Buffer to 5 ng/µl by adding 5 µl of HK5 to 5 µl of HK2. Then, further prepare five concentrations by combining the 5 ng/µl Diluted HK5 with HK2 into final concentrations of 0.2, 0.5, 1.0, 2.0, and 5.0 ng/µl according to the following dilution chart. Then add 2 ul of diluted HK5 at different concentrations to each assay well.
Tube HK5(5 ng/µl) HK2 Resulting HK5 Concentration
1 0.5 µl 24.5 µl 0.1 ng/µ
2 1.0 µl 24.0 µl 0.2 ng/µl
3 1.0 µl 9.0 µl 0.5 ng/µl
4 1.0 µl 4.0 µl 1.0 ng/µl
5 2.0 µl 3.0 µl 2.0 ng/µl
6 4.0 µl 0.0 µl 5.0 ng/µl
I hope this will be useful for you.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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