Application
Western blot
Sample
Drosophila melanogaster Tissue lysate - whole (larval brain)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
5 µg
Specification
larval brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS-Tween 0,1%
Dilution
1/400
Secondary antibody
Name
Non-Abcam antibody was used: anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
larval brain from su(var) 3-9 or su(var)2-5 mutants
Exposure
30 second(s)
Bands
Specific: 17 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
PROF. Laura Ciapponi
Verified customer
提交于 Nov 15 2022