Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (MEFs SV40 transformed)
Specification
MEFs SV40 transformed
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2% triton X100 10 min RT
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Other product details
Dilution
1/200
Incubation time
1 hour(s) and 0 minute(s) · Diluent: blocking agent
Secondary antibody
Name
Non-Abcam antibody was used: goat anti mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Dilution
1/200
Additional data
Additional Notes
H3K9me2 is in red Hoechst staining is in green
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Mark Rochman
Verified customer
提交于 Jul 31 2007